##################################
# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII AccII AccIII AciI AclI
AflII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI AspEI AspI
AvaI AviII AvrII BamHI BbeI
BbrPI BbvCI BcgI BcnI BfrI
BglI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaI BsaWI BseAI BsiEI
BsiWI BsoBI BspDI BspEI BspMI
BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstEII BstPI BstUI
CelII CfoI Cfr10I ClaI CpoI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I FauI FseI
FspI HaeII HapII HhaI HinP1I
HpaII KasI KspI MamI MluI
MroI MspA1I MspI MvnI NaeI
NarI NciI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI PvuII RsrII SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SphI Sse8387I SwaI TaqI
Tth111I TthHB8I XcmI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 29 enzymes) #
###################################
Acc65I 1981 GGTACC G^GTACC
AcyI 2102 GGCGTC G[GA]^CG[CT]C
AflIII 3594 ACATGT A^C[GA][CT]GT
AlwNI 2939 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 1981 GGTACC G^GTACC
BanII 1575 GAGCTC G[GA]GC[CT]^C
BciVI 829 GTATCC GTATCC(6/5)
BsaHI 2102 GGCGTC G[GA]^CG[CT]C
BsmBI 2104 CGTCTC CGTCTC(1/5)
BspLU11I 3594 ACATGT A^CATGT
BstXI 2333 CCAACAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 1212 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoO109I 1212 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoT22I 8 ATGCAT ATGCA^T
HgaI 2103 GCGTC GACGC(5/10)
Hin1I 2102 GGCGTC G[GA]^CG[CT]C
HindIII 2009 AAGCTT A^AGCTT
HpaI 2131 GTTAAC GTT^AAC
HphI 1435 TCACC GGTGA(8/7)
KpnI 1981 GGTACC GGTAC^C
MfeI 2979 CAATTG C^AATTG
MunI 2979 CAATTG C^AATTG
NsiI 8 ATGCAT ATGCA^T
PpuMI 1212 GGGTCCC [GA]G^G[AT]CC[CT]
PstI 565 CTGCAG CTGCA^G
SacI 1575 GAGCTC GAGCT^C
ScaI 794 AGTACT AGT^ACT
StuI 2446 AGGCCT AGG^CCT
XbaI 3160 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AocI 833 CCTCAGG CC^T[ACGT]AGG
1645 CCTGAGG
AspHI 494 GTGCTC G[AT]GC[AT]^C
1575 GAGCTC
BalI 576 TGGCCA TGG^CCA
1151 TGGCCA
BanI 185 GGTGCC G^G[CT][GA]CC
1981 GGTACC
BclI 881 TGATCA T^GATCA
3771 TGATCA
BglII 2194 AGATCT A^GATCT
2929 AGATCT
BmyI 494 GTGCTC G[AGT]GC[ACT]^C
1575 GAGCTC
BseRI 616 CTCCTC GAGGAG(10/8)
1781 CTCCTC
BsgI 1009 GTGCAG GTGCAG(16/14)
3601 GTGCAG
BsiHKAI 494 GTGCTC G[AT]GC[AT]^C
1575 GAGCTC
BsmI 1077 GAATGC GAATGC(1/-1)
1145 GCATTC
Bsp1286I 494 GTGCTC G[AGT]GC[ACT]^C
1575 GAGCTC
Bsp1407I 1663 TGTACA T^GTACA
1814 TGTACA
BsrGI 1663 TGTACA T^GTACA
1814 TGTACA
Bst1107I 238 GTATAC GTA^TAC
1058 GTATAC
BstZ17I 238 GTATAC GTA^TAC
1058 GTATAC
Bsu36I 833 CCTCAGG CC^T[ACGT]AGG
1645 CCTGAGG
Cac8I 574 GCTGGC GC[ACGT]^[ACGT]GC
1632 GCCAGC
DrdI 632 GACGAGCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1797 GACTTCTAAGTC
DsaI 2146 CCATGG C^C[GA][CT]GG
2232 CCATGG
EaeI 576 TGGCCA [CT]^GGCC[GA]
1151 TGGCCA
EarI 1967 CTCTTC CTCTTC(1/4)
2151 GAAGAG
Eco81I 833 CCTCAGG CC^T[ACGT]AGG
1645 CCTGAGG
EcoT14I 2146 CCATGG C^C[AT][AT]GG
2232 CCATGG
FbaI 881 TGATCA T^GATCA
3771 TGATCA
HincII 2131 GTTAAC GT[CT]^[GA]AC
2898 GTCAAC
HindII 2131 GTTAAC GT[CT]^[GA]AC
2898 GTCAAC
Ksp632I 1967 CTCTTC CTCTTC(1/4)
2151 GAAGAG
MluNI 576 TGGCCA TGG^CCA
1151 TGGCCA
MscI 576 TGGCCA TGG^CCA
1151 TGGCCA
MwoI 657 GCTGCCATAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
862 GCCCTTCTTGC
NcoI 2146 CCATGG C^CATGG
2232 CCATGG
NdeI 285 CATATG CA^TATG
3570 CATATG
PflMI 425 CCATCTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
579 CCACATTTTGG
SpeI 514 ACTAGT A^CTAGT
797 ACTAGT
SspBI 1663 TGTACA T^GTACA
1814 TGTACA
StyI 2146 CCATGG C^C[AT][AT]GG
2232 CCATGG
Van91I 425 CCATCTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
579 CCACATTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AccI AcsI AfaI AluI AlwI
ApoI AseI AsnI Asp700I AvaII
BbsI BbvI BfaI BpmI BpuAI
BsaJI BsiYI BslI BsmAI BsmFI
BspHI BsrI BssKI BstF5I BstNI
BstYI Cfr13I DdeI DpnII DraI
EcoRI EcoRII EcoRV Fnu4HI FokI
HaeIII HinfI ItaI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MvaI NdeII NlaIII
NlaIV NspI PleI PshBI RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SspI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII XmnI