##################################
# NO CUT ENZYMES ( 109 enzymes) #
##################################
Acc65I AccII AccIII AclI AflIII
AgeI AhdI AocI Aor51HI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AvrII BamHI BanI BbeI
BbrPI BbvCI BcgI BclI BcnI
BlnI BsaAI BsaBI BsaWI BseAI
BsiEI BsiWI BsmBI BsoBI BspDI
BspEI BspLU11I BsrDI BsrFI BssHII
BssSI Bst1107I BstAPI BstUI BstXI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoRI EcoRV
FbaI FseI HaeII HapII HpaII
KasI KpnI KspI MamI MluI
MroI MspI MvnI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SapI SbfI SexAI SfiI SfoI
SgrAI SmaI SnaBI Sse8387I SwaI
Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AatII 812 GACGTC GACGT^C
AcyI 812 GACGTC G[GA]^CG[CT]C
Alw44I 1220 GTGCAC G^TGCAC
ApaLI 1220 GTGCAC G^TGCAC
AviII 2992 TGCGCA TGC^GCA
BglI 2017 GCCAGCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3467 AGATCT A^GATCT
BsaHI 812 GACGTC G[GA]^CG[CT]C
BsmI 3959 GAATGC GAATGC(1/-1)
BspMI 3566 GCAGGT ACCTGC(4/8)
CfoI 2993 GCGC GCG^C
DraIII 1901 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
FauI 3167 GCGGG CCCGC(4/6)
FspI 2992 TGCGCA TGC^GCA
HgaI 3953 GCGTC GACGC(5/10)
HhaI 2993 GCGC GCG^C
Hin1I 812 GACGTC G[GA]^CG[CT]C
HinP1I 2993 GCGC G^CGC
HpaI 2814 GTTAAC GTT^AAC
NcoI 214 CCATGG C^CATGG
NspI 2977 GCATGC [GA]CATG^[CT]
PflMI 341 CCACCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 3339 AGGTCCC [GA]G^G[AT]CC[CT]
SacI 3445 GAGCTC GAGCT^C
ScaI 1237 AGTACT AGT^ACT
SpeI 1739 ACTAGT A^CTAGT
SphI 2977 GCATGC GCATG^C
StuI 4114 AGGCCT AGG^CCT
Van91I 341 CCACCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1493 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AccI 538 GTAGAC GT^[AC][GT]AC
694 GTAGAC
AflII 777 CTTAAG C^TTAAG
1715 CTTAAG
AlwNI 248 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
3587 CAGGCTCTG
ApaI 467 GGGCCC GGGCC^C
1293 GGGCCC
Asp700I 3413 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3548 GAAGGATTTC
AvaII 872 GGACC G^G[AT]CC
3340 GGTCC
BalI 3520 TGGCCA TGG^CCA
3871 TGGCCA
BbsI 1058 GAAGAC GAAGAC(2/6)
3762 GAAGAC
BciVI 2722 GGATAC GTATCC(6/5)
2071 GTATCC
BfrI 777 CTTAAG C^TTAAG
1715 CTTAAG
BpuAI 1058 GAAGAC GAAGAC(2/6)
3762 GAAGAC
BsaI 315 GGTCTC GGTCTC(1/5)
3569 GGTCTC
BsgI 1856 CTGCAC GTGCAG(16/14)
3099 GTGCAG
Bsp1407I 611 TGTACA T^GTACA
3387 TGTACA
BspHI 233 TCATGA T^CATGA
4272 TCATGA
BsrBI 1447 GAGCGG CCGCTC(-3/-3)
3165 GAGCGG
BsrGI 611 TGTACA T^GTACA
3387 TGTACA
BstBI 2407 TTCGAA TT^CGAA
2791 TTCGAA
BstEII 941 GGTCACC G^GT[ACGT]ACC
4205 GGTAACC
BstPI 941 GGTCACC G^GT[ACGT]ACC
4205 GGTAACC
DsaI 214 CCATGG C^C[GA][CT]GG
1895 CCGTGG
EaeI 3520 TGGCCA [CT]^GGCC[GA]
3871 TGGCCA
EcoO65I 941 GGTCACC G^GT[ACGT]ACC
4205 GGTAACC
HincII 2595 GTCAAC GT[CT]^[GA]AC
2814 GTTAAC
HindII 2595 GTCAAC GT[CT]^[GA]AC
2814 GTTAAC
HindIII 673 AAGCTT A^AGCTT
2556 AAGCTT
MaeII 813 ACGT A^CGT
4277 ACGT
MfeI 1432 CAATTG C^AATTG
2253 CAATTG
MluNI 3520 TGGCCA TGG^CCA
3871 TGGCCA
MscI 3520 TGGCCA TGG^CCA
3871 TGGCCA
MslI 1559 CATTCATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3135 CATCGAAATG
MunI 1432 CAATTG C^AATTG
2253 CAATTG
NspV 2407 TTCGAA TT^CGAA
2791 TTCGAA
RcaI 233 TCATGA T^CATGA
4272 TCATGA
SfuI 2407 TTCGAA TT^CGAA
2791 TTCGAA
SspBI 611 TGTACA T^GTACA
3387 TGTACA
SspI 1181 AATATT AAT^ATT
4187 AATATT
XcmI 3334 CCAAGAGGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3917 CCAGCTGCCCAATGG
XmnI 3413 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3548 GAAGGATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI BanII BbvI BfaI
BlpI BmyI BpmI Bpu1102I BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I CelII Cfr13I
DdeI DpnII DraI DraII EarI
EcoO109I EcoRII EcoT14I EcoT22I Fnu4HI
FokI HaeIII HinfI HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MspA1I MvaI
MwoI NdeII NlaIII NlaIV NsiI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII