##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI ApoI AscI
AseI AsnI Asp718I AspEI AvrII
BbrPI BciVI BclI BfrI BlnI
BsaBI BseAI BsiWI BsmI BspDI
BspEI BspHI BstBI ClaI CpoI
DraI DraIII Eam1105I EcoRI EcoT22I
FbaI FseI HindIII KpnI KspI
MamI MfeI MluI MroI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI ScaI SfuI
SpeI SspI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AccI 5081 GTATAC GT^[AC][GT]AC
Asp700I 1076 GAACTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 139 TGCGCA TGC^GCA
BbeI 3839 GGCGCC GGCGC^C
BglII 1291 AGATCT A^GATCT
BsaWI 3368 TCCGGT [AT]^CCGG[AT]
Bsp1407I 5059 TGTACA T^GTACA
BspLU11I 5050 ACATGT A^CATGT
BsrGI 5059 TGTACA T^GTACA
BssHII 622 GCGCGC G^CGCGC
Bst1107I 5081 GTATAC GTA^TAC
BstAPI 1064 GCAGCACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3020 GGTCACC G^GT[ACGT]ACC
BstPI 3020 GGTCACC G^GT[ACGT]ACC
BstZ17I 5081 GTATAC GTA^TAC
DrdI 4601 GACCCCAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 3020 GGTCACC G^GT[ACGT]ACC
EcoRV 4329 GATATC GAT^ATC
FspI 139 TGCGCA TGC^GCA
HincII 5302 GTTAAC GT[CT]^[GA]AC
HindII 5302 GTTAAC GT[CT]^[GA]AC
HpaI 5302 GTTAAC GTT^AAC
KasI 3839 GGCGCC G^GCGCC
NarI 3839 GGCGCC GG^CGCC
PshAI 4005 GACTGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SexAI 751 ACCTGGT A^CC[AT]GGT
SfiI 1499 GGCCTACGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 3839 GGCGCC GGC^GCC
SgrAI 2344 CGCCGGCG C[GA]^CCGG[CT]G
SnaBI 3261 TACGTA TAC^GTA
SphI 719 GCATGC GCATG^C
SspBI 5059 TGTACA T^GTACA
XmnI 1076 GAACTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcyI 2427 GACGCC G[GA]^CG[CT]C
3839 GGCGCC
Alw44I 4900 GTGCAC G^TGCAC
4907 GTGCAC
Aor51HI 306 AGCGCT AGC^GCT
1403 AGCGCT
ApaLI 4900 GTGCAC G^TGCAC
4907 GTGCAC
AspI 1788 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3909 GACGGGGTC
BbvCI 3436 CCTCAGC CCTCAGC(-5/-2)
230 GCTGAGG
BcgI 581 CGATAGCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2887 GCAACGCCCTCG
BfaI 927 CTAG C^TAG
4800 CTAG
BsaAI 2709 TACGTG [CT]AC^GT[GA]
3261 TACGTA
BsaHI 2427 GACGCC G[GA]^CG[CT]C
3839 GGCGCC
Eco47III 306 AGCGCT AGC^GCT
1403 AGCGCT
Hin1I 2427 GACGCC G[GA]^CG[CT]C
3839 GGCGCC
MaeI 927 CTAG C^TAG
4800 CTAG
PaeR7I 501 CTCGAG C^TCGAG
759 CTCGAG
PflFI 1788 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3909 GACGGGGTC
SapI 770 GAAGAGC GCTCTTC(1/4)
2418 GAAGAGC
SbfI 1322 CCTGCAGG CCTGCA^GG
4696 CCTGCAGG
Sse8387I 1322 CCTGCAGG CCTGCA^GG
4696 CCTGCAGG
Tth111I 1788 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3909 GACGGGGTC
XhoI 501 CTCGAG C^TCGAG
759 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 136 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII BalI BamHI BanI
BanII BbsI BbvI BcnI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrDI BsrFI BsrI BssKI BssSI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EagI EarI EclXI Eco52I
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI