##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
Acc65I AclI Alw44I AocI ApaLI
AscI Asp700I Asp718I AviII AvrII
BamHI BcgI BglII BlnI BsiWI
Bsp1407I BspDI BspLU11I BsrGI BstBI
BstEII BstPI BstYI Bsu36I ClaI
DraIII Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HindIII
HpaI KpnI MfeI MflI MunI
NcoI NheI NotI NsiI NspV
PacI PmeI Psp1406I PvuI SalI
ScaI SfiI SfuI SpeI SspBI
SwaI XbaI XhoII XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 556 GACGTC GACGT^C
AccIII 4235 TCCGGA T^CCGGA
AflII 1001 CTTAAG C^TTAAG
AflIII 493 ACGCGT A^C[GA][CT]GT
AgeI 841 ACCGGT A^CCGGT
AhdI 265 GACTCGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 4507 AGCGCT AGC^GCT
AseI 4780 ATTAAT AT^TAAT
AsnI 4780 ATTAAT AT^TAAT
AspEI 265 GACTCGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BfrI 1001 CTTAAG C^TTAAG
BsaBI 1525 GATTAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 4235 TCCGGA T^CCGGA
BsmI 4922 GAATGC GAATGC(1/-1)
BspEI 4235 TCCGGA T^CCGGA
BspHI 1758 TCATGA T^CATGA
BsrBI 2150 GAGCGG CCGCTC(-3/-3)
BssSI 4989 CTCGTG CACGAG(-5/-1)
Bst1107I 3114 GTATAC GTA^TAC
BstZ17I 3114 GTATAC GTA^TAC
CpoI 1284 CGGTCCG CG^G[AT]CCG
Eam1105I 265 GACTCGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 4507 AGCGCT AGC^GCT
MamI 1525 GATTAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 493 ACGCGT A^CGCGT
MroI 4235 TCCGGA T^CCGGA
NdeI 1511 CATATG CA^TATG
NruI 2722 TCGCGA TCG^CGA
PinAI 841 ACCGGT A^CCGGT
PshBI 4780 ATTAAT AT^TAAT
RcaI 1758 TCATGA T^CATGA
RsrII 1284 CGGTCCG CG^G[AT]CCG
SbfI 1958 CCTGCAGG CCTGCA^GG
SexAI 2244 ACCTGGT A^CC[AT]GGT
SnaBI 4624 TACGTA TAC^GTA
Sse8387I 1958 CCTGCAGG CCTGCA^GG
SspI 3216 AATATT AAT^ATT
XcmI 2346 CCAACTTCCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 3114 GTATAC GT^[AC][GT]AC
4587 GTAGAC
AspI 2570 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3827 GACCCTGTC
BbeI 1355 GGCGCC GGCGC^C
3123 GGCGCC
BbrPI 329 CACGTG CAC^GTG
872 CACGTG
BciVI 1294 GGATAC GTATCC(6/5)
4979 GGATAC
BsiEI 622 CGGCCG CG[GA][CT]^CG
768 CGGCCG
BsrDI 3797 CATTGC GCAATG(2/0)
1415 GCAATG
BssHII 3725 GCGCGC G^CGCGC
4439 GCGCGC
BstAPI 1213 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2362 GCAGCAGCTGC
DraI 3699 TTTAAA TTT^AAA
4481 TTTAAA
EagI 622 CGGCCG C^GGCCG
768 CGGCCG
EclXI 622 CGGCCG C^GGCCG
768 CGGCCG
Eco52I 622 CGGCCG C^GGCCG
768 CGGCCG
KasI 1355 GGCGCC G^GCGCC
3123 GGCGCC
KspI 661 CCGCGG CCGC^GG
677 CCGCGG
NarI 1355 GGCGCC GG^CGCC
3123 GGCGCC
PaeR7I 716 CTCGAG C^TCGAG
2007 CTCGAG
PflFI 2570 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3827 GACCCTGTC
PmaCI 329 CACGTG CAC^GTG
872 CACGTG
PmlI 329 CACGTG CAC^GTG
872 CACGTG
PshAI 3587 GACTCACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4000 GACTATGGTC
SacII 661 CCGCGG CCGC^GG
677 CCGCGG
SapI 1636 GAAGAGC GCTCTTC(1/4)
2485 GCTCTTC
SfoI 1355 GGCGCC GGC^GCC
3123 GGCGCC
SgrAI 840 CACCGGTG C[GA]^CCGG[CT]G
1726 CACCGGCG
SphI 1143 GCATGC GCATG^C
4073 GCATGC
Tth111I 2570 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3827 GACCCTGTC
XhoI 716 CTCGAG C^TCGAG
2007 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 133 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApaI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvCI BbvI BclI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstXI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DrdI
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XmaCI XmaI