##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
Acc65I AocI Aor51HI ApaI AscI
AseI AsnI Asp718I AviII BbrPI
BcgI BsaAI BsaBI BsiEI BsiWI
BspDI BssHII BssSI Bsu36I ClaI
CpoI EagI EclXI Eco47III Eco52I
Eco81I EcoRI FseI FspI HincII
HindII HpaI KpnI KspI MamI
MfeI MluI MunI NaeI NgoMIV
NheI NotI NruI PacI PaeR7I
PmaCI PmeI PmlI PshAI PshBI
PvuI RsrII SacII SalI SbfI
SfiI SmaI SnaBI SpeI Sse8387I
SwaI XbaI XcmI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AatII 3296 GACGTC GACGT^C
AccIII 3134 TCCGGA T^CCGGA
AclI 2455 AACGTT AA^CGTT
AflII 1904 CTTAAG C^TTAAG
AflIII 1036 ACATGT A^C[GA][CT]GT
AgeI 3453 ACCGGT A^CCGGT
AhdI 3585 GACAGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1560 GTGCAC G^TGCAC
ApaLI 1560 GTGCAC G^TGCAC
Asp700I 4160 GAAATAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 3585 GACAGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 347 CCCGAG C^[CT]CG[GA]G
AvrII 4305 CCTAGG C^CTAGG
BalI 452 TGGCCA TGG^CCA
BamHI 225 GGATCC G^GATCC
BbsI 3574 GAAGAC GAAGAC(2/6)
BbvCI 4719 GCTGAGG CCTCAGC(-5/-2)
BciVI 1192 GGATAC GTATCC(6/5)
BclI 2182 TGATCA T^GATCA
BfrI 1904 CTTAAG C^TTAAG
BlnI 4305 CCTAGG C^CTAGG
BlpI 4262 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 4262 GCTCAGC GC^T[ACGT]AGC
BpuAI 3574 GAAGAC GAAGAC(2/6)
BseAI 3134 TCCGGA T^CCGGA
BsoBI 347 CCCGAG C^[CT]CG[GA]G
BspEI 3134 TCCGGA T^CCGGA
BspHI 1606 TCATGA T^CATGA
BspLU11I 1036 ACATGT A^CATGT
BspMI 2312 GCAGGT ACCTGC(4/8)
BsrBI 4260 CCGCTC CCGCTC(-3/-3)
Bst1107I 1304 GTATAC GTA^TAC
BstBI 1171 TTCGAA TT^CGAA
BstEII 3661 GGTCACC G^GT[ACGT]ACC
BstPI 3661 GGTCACC G^GT[ACGT]ACC
BstXI 4704 CCAGCTACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1304 GTATAC GTA^TAC
CelII 4262 GCTCAGC GC^T[ACGT]AGC
DrdI 2656 GACCTCCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 3585 GACAGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 3661 GGTCACC G^GT[ACGT]ACC
FbaI 2182 TGATCA T^GATCA
MluNI 452 TGGCCA TGG^CCA
MroI 3134 TCCGGA T^CCGGA
MscI 452 TGGCCA TGG^CCA
NdeI 1543 CATATG CA^TATG
NspV 1171 TTCGAA TT^CGAA
PinAI 3453 ACCGGT A^CCGGT
Psp1406I 2455 AACGTT AA^CGTT
RcaI 1606 TCATGA T^CATGA
ScaI 1935 AGTACT AGT^ACT
SexAI 3287 ACCTGGT A^CC[AT]GGT
SfuI 1171 TTCGAA TT^CGAA
SgrAI 111 CGCCGGTG C[GA]^CCGG[CT]G
SphI 3863 GCATGC GCATG^C
SspI 746 AATATT AAT^ATT
StuI 249 AGGCCT AGG^CCT
XmnI 4160 GAAATAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccII 2508 CGCG CG^CG
2930 CGCG
AspI 2274 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4808 GACCCTGTC
BanI 109 GGCGCC G^G[CT][GA]CC
2349 GGCGCC
BbeI 109 GGCGCC GGCGC^C
2349 GGCGCC
BglII 2546 AGATCT A^GATCT
4252 AGATCT
BsaI 4530 GAGACC GGTCTC(1/5)
4806 GAGACC
BsmBI 2256 CGTCTC CGTCTC(1/5)
3298 CGTCTC
BsmI 286 GAATGC GAATGC(1/-1)
4215 GAATGC
Bsp1407I 3238 TGTACA T^GTACA
4009 TGTACA
BsrFI 112 GCCGGT [GA]^CCGG[CT]
3453 ACCGGT
BsrGI 3238 TGTACA T^GTACA
4009 TGTACA
BstAPI 2291 GCAGGAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2403 GCAGATGCTGC
BstUI 2508 CGCG CG^CG
2930 CGCG
Cfr10I 112 GCCGGT [GA]^CCGG[CT]
3453 ACCGGT
DraI 807 TTTAAA TTT^AAA
4067 TTTAAA
DraIII 3259 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
4425 CACAGTGTG
EaeI 95 TGGCCG [CT]^GGCC[GA]
452 TGGCCA
EcoNI 1583 CCTCTGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3207 CCTTCGCAAGG
EcoT22I 319 ATGCAT ATGCA^T
604 ATGCAT
HaeII 109 GGCGCC [GA]GCGC^[CT]
2349 GGCGCC
HgaI 3193 GACGC GACGC(5/10)
524 GCGTC
HindIII 1090 AAGCTT A^AGCTT
1570 AAGCTT
KasI 109 GGCGCC G^GCGCC
2349 GGCGCC
MvnI 2508 CGCG CG^CG
2930 CGCG
NarI 109 GGCGCC GG^CGCC
2349 GGCGCC
NsiI 319 ATGCAT ATGCA^T
604 ATGCAT
PflFI 2274 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4808 GACCCTGTC
PflMI 831 CCAGAGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4424 CCACAGTGTGG
PvuII 868 CAGCTG CAG^CTG
3796 CAGCTG
SapI 1122 GAAGAGC GCTCTTC(1/4)
1827 GAAGAGC
SfoI 109 GGCGCC GGC^GCC
2349 GGCGCC
SspBI 3238 TGTACA T^GTACA
4009 TGTACA
Tth111I 2274 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4808 GACCCTGTC
Van91I 831 CCAGAGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4424 CCACAGTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaII BanII BbvI BcnI BfaI
BglI BmyI BpmI BsaHI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrDI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EarI EcoO109I EcoRII
EcoRV EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII