##################################
# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspI AvaI
AviII AvrII BamHI BbeI BbrPI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaHI BseAI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspLU11I BsrGI BssHII BssSI
Bst1107I BstBI BstUI BstXI BstZ17I
Bsu36I CelII ClaI CpoI DrdI
DsaI EagI EclXI Eco47III Eco52I
Eco81I EcoRV FauI FseI FspI
HaeII HgaI Hin1I HindIII HpaI
KasI KpnI KspI MfeI MluI
MroI MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RsrII SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI Tth111I XcmI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AfaI 183 GTAC GT^AC
AgeI 2219 ACCGGT A^CCGGT
AhdI 479 GACGACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 2454 CAGGTTCTG CAG[ACGT][ACGT][ACGT]^CTG
AspEI 479 GACGACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 1899 GGCACC G^G[CT][GA]CC
BbsI 838 GTCTTC GAAGAC(2/6)
BbvCI 1372 CCTCAGC CCTCAGC(-5/-2)
BcgI 1204 CGACCCTGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 762 GTATCC GTATCC(6/5)
BcnI 1556 CCGGG CC^[GC]GG
BpuAI 838 GTCTTC GAAGAC(2/6)
BsaBI 2486 GATCAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2219 ACCGGT [AT]^CCGG[AT]
BspHI 607 TCATGA T^CATGA
BspMI 1474 ACCTGC ACCTGC(4/8)
BsrBI 1919 GAGCGG CCGCTC(-3/-3)
BsrFI 2219 ACCGGT [GA]^CCGG[CT]
BstEII 2215 GGTAACC G^GT[ACGT]ACC
BstPI 2215 GGTAACC G^GT[ACGT]ACC
CfoI 990 GCGC GCG^C
Cfr10I 2219 ACCGGT [GA]^CCGG[CT]
DraI 1004 TTTAAA TTT^AAA
Eam1105I 479 GACGACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 2215 GGTAACC G^GT[ACGT]ACC
EcoRI 1525 GAATTC G^AATTC
HhaI 990 GCGC GCG^C
HinP1I 990 GCGC G^CGC
MaeII 474 ACGT A^CGT
MamI 2486 GATCAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 2582 CCGCTG C[AC]G^C[GT]G
NciI 1556 CCGGG CC^[GC]GG
PinAI 2219 ACCGGT A^CCGGT
RcaI 607 TCATGA T^CATGA
RsaI 183 GTAC GT^AC
SacI 750 GAGCTC GAGCT^C
ScaI 182 AGTACT AGT^ACT
SspI 1722 AATATT AAT^ATT
XbaI 742 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
BalI 2155 TGGCCA TGG^CCA
2461 TGGCCA
BanII 750 GAGCTC G[GA]GC[CT]^C
1892 GGGCTC
BclI 2272 TGATCA T^GATCA
2485 TGATCA
BsaI 1328 GAGACC GGTCTC(1/5)
2578 GAGACC
BseRI 551 GAGGAG GAGGAG(10/8)
1229 GAGGAG
BsmI 586 GCATTC GAATGC(1/-1)
1296 GCATTC
EaeI 2155 TGGCCA [CT]^GGCC[GA]
2461 TGGCCA
EarI 618 CTCTTC CTCTTC(1/4)
1361 CTCTTC
EcoNI 728 CCTGGTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1372 CCTCAGCGAGG
EcoT14I 315 CCTTGG C^C[AT][AT]GG
1511 CCTTGG
EcoT22I 300 ATGCAT ATGCA^T
584 ATGCAT
FbaI 2272 TGATCA T^GATCA
2485 TGATCA
HapII 1556 CCGG C^CGG
2220 CCGG
HpaII 1556 CCGG C^CGG
2220 CCGG
Ksp632I 618 CTCTTC CTCTTC(1/4)
1361 CTCTTC
MluNI 2155 TGGCCA TGG^CCA
2461 TGGCCA
MscI 2155 TGGCCA TGG^CCA
2461 TGGCCA
MspI 1556 CCGG C^CGG
2220 CCGG
NsiI 300 ATGCAT ATGCA^T
584 ATGCAT
PflMI 411 CCAGTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2332 CCAGCATGTGG
PpuMI 784 GGGACCC [GA]G^G[AT]CC[CT]
1083 GGGTCCT
PstI 1429 CTGCAG CTGCA^G
2532 CTGCAG
SmlI 189 CTCAAG C^T[CT][GA]AG
1580 CTTGAG
StyI 315 CCTTGG C^C[AT][AT]GG
1511 CCTTGG
TfiI 845 GATTC G^A[AT]TC
2260 GATTC
Van91I 411 CCAGTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2332 CCAGCATGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
Asp700I AspHI AvaII BbvI BfaI
BmyI BpmI BsaJI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrDI BsrI BssKI BstAPI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraII DraIII EcoO109I EcoRII
Fnu4HI FokI HaeIII HincII HindII
HinfI HphI ItaI MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI Sau3AI Sau96I
ScrFI SfaNI SfcI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI