##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
Alw44I ApaLI AscI Asp700I Asp718I
AspI AvrII BbrPI BfrI BlnI
BsaAI BsaBI BseAI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
EagI EclXI Eco52I EcoO65I EcoRV
EcoT22I FseI HindIII HpaI KpnI
MamI MfeI MluI MroI MunI
NdeI NheI NotI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RcaI RsrII SapI SfiI SfuI
SgrAI SnaBI SpeI SspBI SspI
StuI SwaI Tth111I XbaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 783 GACGTC GACGT^C
AcsI 1463 GAATTC [GA]^AATT[CT]
AhdI 3411 GACCCTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2035 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1483 AGCGCT AGC^GCT
ApoI 1463 GAATTC [GA]^AATT[CT]
AspEI 3411 GACCCTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 319 TGCGCA TGC^GCA
BamHI 1275 GGATCC G^GATCC
BbeI 1248 GGCGCC GGCGC^C
BcgI 994 GCAAGATGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 106 TGATCA T^GATCA
BglI 2072 GCCCCCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 88 TCCGGT [AT]^CCGG[AT]
BsrFI 1312 GCCGGC [GA]^CCGG[CT]
Bsu36I 2035 CCTCAGG CC^T[ACGT]AGG
Cfr10I 1312 GCCGGC [GA]^CCGG[CT]
DraI 3499 TTTAAA TTT^AAA
Eam1105I 3411 GACCCTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1483 AGCGCT AGC^GCT
Eco81I 2035 CCTCAGG CC^T[ACGT]AGG
EcoRI 1463 GAATTC G^AATTC
FbaI 106 TGATCA T^GATCA
FspI 319 TGCGCA TGC^GCA
KasI 1248 GGCGCC G^GCGCC
NaeI 1312 GCCGGC GCC^GGC
NarI 1248 GGCGCC GG^CGCC
NgoMIV 1312 GCCGGC G^CCGGC
NruI 1043 TCGCGA TCG^CGA
PflMI 863 CCAGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 1591 GAGCTC GAGCT^C
SalI 2248 GTCGAC G^TCGAC
SbfI 2575 CCTGCAGG CCTGCA^GG
SexAI 324 ACCTGGT A^CC[AT]GGT
SfoI 1248 GGCGCC GGC^GCC
SphI 775 GCATGC GCATG^C
Sse8387I 2575 CCTGCAGG CCTGCA^GG
Van91I 863 CCAGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 667 CCATCAACCACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AflIII 922 ACATGT A^C[GA][CT]GT
2131 ACATGT
AseI 2882 ATTAAT AT^TAAT
3017 ATTAAT
AsnI 2882 ATTAAT AT^TAAT
3017 ATTAAT
BciVI 2687 GTATCC GTATCC(6/5)
3283 GTATCC
BglII 520 AGATCT A^GATCT
1291 AGATCT
BspLU11I 922 ACATGT A^CATGT
2131 ACATGT
DrdI 402 GACGCTGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
654 GACTGCTGGGTC
HgaI 402 GACGC GACGC(5/10)
884 GCGTC
HincII 336 GTTGAC GT[CT]^[GA]AC
2248 GTCGAC
HindII 336 GTTGAC GT[CT]^[GA]AC
2248 GTCGAC
KspI 294 CCGCGG CCGC^GG
1244 CCGCGG
MaeII 202 ACGT A^CGT
784 ACGT
PshBI 2882 ATTAAT AT^TAAT
3017 ATTAAT
PvuII 1651 CAGCTG CAG^CTG
3491 CAGCTG
SacII 294 CCGCGG CCGC^GG
1244 CCGCGG
SmlI 1389 CTTGAG C^T[CT][GA]AG
2305 CTCAAG
TfiI 2310 GAATC G^A[AT]TC
2928 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI AlwI AlwNI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrDI
BsrI BssKI BssSI BstAPI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmaI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmaCI
XmaI