##################################
# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII AccIII AcyI AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI AspEI AviII BbeI
BbrPI BcgI BciVI BglI BsaAI
BsaHI BsaWI BseAI BsiEI BsiWI
Bsp1407I BspDI BspEI BspHI BsrFI
BsrGI BssHII Bst1107I BstBI BstZ17I
Cfr10I ClaI CpoI DraIII EagI
Eam1105I EclXI Eco47III Eco52I EcoRV
FseI FspI HgaI Hin1I HindIII
KasI KspI MluI MroI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI PvuI RcaI RsrII SacII
SalI SapI SbfI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SwaI Van91I
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Acc65I 2391 GGTACC G^GTACC
AccI 2489 GTAGAC GT^[AC][GT]AC
AccII 89 CGCG CG^CG
AclI 1548 AACGTT AA^CGTT
AflIII 1470 ACATGT A^C[GA][CT]GT
Asp700I 3227 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2391 GGTACC G^GTACC
AspI 3297 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 3968 CCCGAG C^[CT]CG[GA]G
BamHI 3320 GGATCC G^GATCC
BbvCI 2151 CCTCAGC CCTCAGC(-5/-2)
BclI 361 TGATCA T^GATCA
BsaBI 545 GATCTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 468 CTGCAC GTGCAG(16/14)
BsmI 307 GAATGC GAATGC(1/-1)
BsoBI 3968 CCCGAG C^[CT]CG[GA]G
BspLU11I 1470 ACATGT A^CATGT
BsrBI 1735 GAGCGG CCGCTC(-3/-3)
BsrDI 497 CATTGC GCAATG(2/0)
BstEII 1669 GGTGACC G^GT[ACGT]ACC
BstPI 1669 GGTGACC G^GT[ACGT]ACC
BstUI 89 CGCG CG^CG
DraI 3851 TTTAAA TTT^AAA
DrdI 1235 GACAGACCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1669 GGTGACC G^GT[ACGT]ACC
EcoRI 1401 GAATTC G^AATTC
FbaI 361 TGATCA T^GATCA
HpaI 3136 GTTAAC GTT^AAC
KpnI 2391 GGTACC GGTAC^C
MamI 545 GATCTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 89 CGCG CG^CG
NcoI 589 CCATGG C^CATGG
PflFI 3297 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 3884 GGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 1548 AACGTT AA^CGTT
PstI 1379 CTGCAG CTGCA^G
SexAI 514 ACCAGGT A^CC[AT]GGT
SspI 3584 AATATT AAT^ATT
StuI 2288 AGGCCT AGG^CCT
Tth111I 3297 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2713 TCTAGA T^CTAGA
XmnI 3227 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AflII 2844 CTTAAG C^TTAAG
2861 CTTAAG
AocI 1110 CCTCAGG CC^T[ACGT]AGG
1317 CCTGAGG
AspHI 1686 GAGCTC G[AT]GC[AT]^C
3076 GAGCTC
AvrII 1406 CCTAGG C^CTAGG
2285 CCTAGG
BfrI 2844 CTTAAG C^TTAAG
2861 CTTAAG
BglII 2850 AGATCT A^GATCT
3954 AGATCT
BlnI 1406 CCTAGG C^CTAGG
2285 CCTAGG
BlpI 1627 GCTGAGC GC^T[ACGT]AGC
3636 GCTTAGC
BpmI 1221 CTCCAG CTGGAG(16/14)
1767 CTGGAG
Bpu1102I 1627 GCTGAGC GC^T[ACGT]AGC
3636 GCTTAGC
BsiHKAI 1686 GAGCTC G[AT]GC[AT]^C
3076 GAGCTC
BsmBI 473 CGTCTC CGTCTC(1/5)
2223 GAGACG
Bsu36I 1110 CCTCAGG CC^T[ACGT]AGG
1317 CCTGAGG
CelII 1627 GCTGAGC GC^T[ACGT]AGC
3636 GCTTAGC
DraII 2505 AGGGCCT [GA]G^G[ACGT]CC[CT]
3884 GGGACCT
DsaI 208 CCACGG C^C[GA][CT]GG
589 CCATGG
Eco81I 1110 CCTCAGG CC^T[ACGT]AGG
1317 CCTGAGG
EcoO109I 2505 AGGGCCT [GA]G^G[ACGT]CC[CT]
3884 GGGACCT
EcoT22I 2837 ATGCAT ATGCA^T
3601 ATGCAT
HaeII 181 GGCGCT [GA]GCGC^[CT]
189 GGCGCT
MfeI 2596 CAATTG C^AATTG
3003 CAATTG
MunI 2596 CAATTG C^AATTG
3003 CAATTG
NsiI 2837 ATGCAT ATGCA^T
3601 ATGCAT
SacI 1686 GAGCTC GAGCT^C
3076 GAGCTC
TaqI 2257 TCGA T^CGA
2978 TCGA
TthHB8I 2257 TCGA T^CGA
2978 TCGA
XcmI 1217 CCAACTCCAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1732 CCAGAGCGGGCGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AvaII BalI BanI
BanII BbsI BbvI BcnI BfaI
BmyI BpuAI BsaI BsaJI BseRI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrI BssKI BssSI BstAPI
BstF5I BstNI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII EaeI
EarI EcoNI EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PvuII
RsaI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SmlI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XhoII