##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AhdI AseI AsnI Asp700I Asp718I
AspEI AvrII BamHI BciVI BclI
BfrI BlnI BsaBI BseAI BsiWI
BsmI BspDI BspEI BspHI BspLU11I
BsrDI Bst1107I BstBI BstZ17I ClaI
DraIII Eam1105I EcoRI EcoRV EcoT22I
FbaI FseI HincII HindII HindIII
HpaI KpnI MamI MfeI MluI
MroI MunI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PmeI PshAI PshBI Psp1406I
PvuI RcaI SalI SapI ScaI
SexAI SfuI SgrAI SnaBI SpeI
SwaI XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AcsI 3538 AAATTC [GA]^AATT[CT]
AgeI 3317 ACCGGT A^CCGGT
ApoI 3538 AAATTC [GA]^AATT[CT]
AscI 2436 GGCGCGCC GG^CGCGCC
AspI 306 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 356 TGCGCA TGC^GCA
BalI 2156 TGGCCA TGG^CCA
BbsI 2114 GAAGAC GAAGAC(2/6)
BglII 1573 AGATCT A^GATCT
BpuAI 2114 GAAGAC GAAGAC(2/6)
BsaI 365 GGTCTC GGTCTC(1/5)
Bsp1407I 3419 TGTACA T^GTACA
BsrGI 3419 TGTACA T^GTACA
BssSI 987 CACGAG CACGAG(-5/-1)
BstAPI 1940 GCACGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 266 GGTCACC G^GT[ACGT]ACC
BstPI 266 GGTCACC G^GT[ACGT]ACC
CpoI 2205 CGGTCCG CG^G[AT]CCG
EarI 1719 CTCTTC CTCTTC(1/4)
EcoNI 56 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 266 GGTCACC G^GT[ACGT]ACC
FspI 356 TGCGCA TGC^GCA
Ksp632I 1719 CTCTTC CTCTTC(1/4)
MluNI 2156 TGGCCA TGG^CCA
MscI 2156 TGGCCA TGG^CCA
NspI 359 GCATGC [GA]CATG^[CT]
PflFI 306 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 3317 ACCGGT A^CCGGT
PvuII 1608 CAGCTG CAG^CTG
RsrII 2205 CGGTCCG CG^G[AT]CCG
SacI 534 GAGCTC GAGCT^C
SbfI 1331 CCTGCAGG CCTGCA^GG
SfiI 762 GGCCTGCACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 359 GCATGC GCATG^C
Sse8387I 1331 CCTGCAGG CCTGCA^GG
SspBI 3419 TGTACA T^GTACA
SspI 4254 AATATT AAT^ATT
Tth111I 306 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3548 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AccI 851 GTCTAC GT^[AC][GT]AC
3900 GTAGAC
Alw44I 1551 GTGCAC G^TGCAC
1938 GTGCAC
AocI 1350 CCTAAGG CC^T[ACGT]AGG
4001 CCTGAGG
ApaLI 1551 GTGCAC G^TGCAC
1938 GTGCAC
BbvCI 773 CCTCAGC CCTCAGC(-5/-2)
1768 CCTCAGC
BfaI 2971 CTAG C^TAG
3549 CTAG
BsaWI 871 TCCGGT [AT]^CCGG[AT]
3317 ACCGGT
BspMI 141 ACCTGC ACCTGC(4/8)
2626 ACCTGC
Bsu36I 1350 CCTAAGG CC^T[ACGT]AGG
4001 CCTGAGG
EagI 979 CGGCCG C^GGCCG
2785 CGGCCG
EclXI 979 CGGCCG C^GGCCG
2785 CGGCCG
Eco52I 979 CGGCCG C^GGCCG
2785 CGGCCG
Eco81I 1350 CCTAAGG CC^T[ACGT]AGG
4001 CCTGAGG
MaeI 2971 CTAG C^TAG
3549 CTAG
NaeI 947 GCCGGC GCC^GGC
3834 GCCGGC
NgoMIV 947 GCCGGC G^CCGGC
3834 GCCGGC
SmlI 471 CTTGAG C^T[CT][GA]AG
2977 CTCAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI Aor51HI ApaI
AspHI AvaI AvaII BanI BanII
BbeI BbrPI BbvI BcgI BcnI
BglI BlpI BmyI BpmI Bpu1102I
BsaAI BsaHI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BsrBI
BsrFI BsrI BssHII BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DrdI DsaI
EaeI Eco47III EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
KspI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NdeII NlaIII NlaIV PflMI
PleI PmaCI PmlI PpuMI PstI
RsaI SacII Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmaI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII XmaCI XmaI