##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AgeI Aor51HI ApaI AscI AviII
AvrII BbeI BbrPI BcgI BlnI
BsaHI BsaWI BseAI BsiEI BsiWI
BspDI BspEI BsrBI BsrFI BssHII
BssSI BstBI BstUI CfoI Cfr10I
ClaI CpoI DraIII DrdI EagI
EclXI Eco47III Eco52I FseI FspI
HaeII HhaI Hin1I HinP1I KasI
KspI MluI MroI MvnI NaeI
NarI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PvuI RsrII SacI SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI Sse8387I SwaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
Acc65I 2749 GGTACC G^GTACC
AflII 3461 CTTAAG C^TTAAG
AhdI 1040 GACCCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2909 GTGCAC G^TGCAC
AocI 275 CCTGAGG CC^T[ACGT]AGG
ApaLI 2909 GTGCAC G^TGCAC
AseI 3599 ATTAAT AT^TAAT
AsnI 3599 ATTAAT AT^TAAT
Asp718I 2749 GGTACC G^GTACC
AspEI 1040 GACCCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 2022 CTCGGG C^[CT]CG[GA]G
BanII 1928 GAGCCC G[GA]GC[CT]^C
BbsI 248 GAAGAC GAAGAC(2/6)
BbvCI 2181 CCTCAGC CCTCAGC(-5/-2)
BciVI 1027 GTATCC GTATCC(6/5)
BclI 4753 TGATCA T^GATCA
BcnI 1355 CCGGG CC^[GC]GG
BfrI 3461 CTTAAG C^TTAAG
BglI 1802 GCCAAAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1907 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1907 GCTGAGC GC^T[ACGT]AGC
BpuAI 248 GAAGAC GAAGAC(2/6)
BsaAI 537 CACGTA [CT]AC^GT[GA]
BsaBI 4748 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 673 GAGACC GGTCTC(1/5)
BsmBI 757 GAGACG CGTCTC(1/5)
BsmFI 619 GTCCC GGGAC(10/14)
BsoBI 2022 CTCGGG C^[CT]CG[GA]G
Bst1107I 4697 GTATAC GTA^TAC
BstEII 2074 GGTTACC G^GT[ACGT]ACC
BstPI 2074 GGTTACC G^GT[ACGT]ACC
BstXI 36 CCACTCTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 4697 GTATAC GTA^TAC
Bsu36I 275 CCTGAGG CC^T[ACGT]AGG
CelII 1907 GCTGAGC GC^T[ACGT]AGC
DraII 2032 AGGGCCT [GA]G^G[ACGT]CC[CT]
Eam1105I 1040 GACCCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 275 CCTGAGG CC^T[ACGT]AGG
EcoNI 4441 CCTTAAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 2032 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 2074 GGTTACC G^GT[ACGT]ACC
FauI 2385 CCCGC CCCGC(4/6)
FbaI 4753 TGATCA T^GATCA
HapII 1355 CCGG C^CGG
HgaI 5059 GACGC GACGC(5/10)
HpaI 5097 GTTAAC GTT^AAC
HpaII 1355 CCGG C^CGG
KpnI 2749 GGTACC GGTAC^C
MamI 4748 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3118 CAATTG C^AATTG
MspI 1355 CCGG C^CGG
MunI 3118 CAATTG C^AATTG
NciI 1355 CCGGG CC^[GC]GG
NdeI 2010 CATATG CA^TATG
PshBI 3599 ATTAAT AT^TAAT
SapI 3387 GCTCTTC GCTCTTC(1/4)
TaqI 256 TCGA T^CGA
TthHB8I 256 TCGA T^CGA
XbaI 1259 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AciI 2315 CCGC CCGC(-3/-1)
2386 CCGC
AlwNI 380 CAGGTACTG CAG[ACGT][ACGT][ACGT]^CTG
708 CAGAGTCTG
Asp700I 2306 GAAAGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4149 GAATCCCTTC
AvaII 602 GGACC G^G[AT]CC
1968 GGACC
BamHI 737 GGATCC G^GATCC
1612 GGATCC
BseRI 353 GAGGAG GAGGAG(10/8)
4548 GAGGAG
BsgI 1347 GTGCAG GTGCAG(16/14)
1563 GTGCAG
EcoRV 806 GATATC GAT^ATC
2055 GATATC
EcoT22I 75 ATGCAT ATGCA^T
3101 ATGCAT
MspA1I 471 CAGCTG C[AC]G^C[GT]G
3570 CAGCTG
NcoI 1879 CCATGG C^CATGG
3155 CCATGG
NsiI 75 ATGCAT ATGCA^T
3101 ATGCAT
PvuII 471 CAGCTG CAG^CTG
3570 CAGCTG
ScaI 2462 AGTACT AGT^ACT
3683 AGTACT
SmlI 852 CTCAAG C^T[CT][GA]AG
3461 CTTAAG
SphI 1687 GCATGC GCATG^C
3327 GCATGC
StuI 2826 AGGCCT AGG^CCT
4552 AGGCCT
XmnI 2306 GAAAGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4149 GAATCCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AccI AcsI AfaI AflIII AluI
AlwI ApoI AspHI AspI BalI
BanI BbvI BfaI BglII BmyI
BpmI BsaJI BsiHKAI BsiYI BslI
BsmAI BsmI Bsp1286I Bsp1407I BspHI
BspLU11I BspMI BsrDI BsrGI BsrI
BssKI BstAPI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraI
DsaI EaeI EarI EcoRI EcoRII
EcoT14I Fnu4HI FokI HaeIII HincII
HindII HindIII HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MvaI MwoI NdeII
NlaIII NlaIV NspI PflFI PflMI
PleI PstI RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SpeI
SspBI SspI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
Van91I XcmI XhoII