##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AclI AflII AgeI AhdI AocI
AseI AsnI Asp700I AspEI BfrI
BsiWI Bsp1407I BspDI BsrGI Bst1107I
BstBI BstZ17I Bsu36I ClaI CpoI
Eam1105I Eco81I EcoRV EcoT22I HincII
HindII HindIII HpaI MluI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshBI
Psp1406I RsrII SalI SbfI ScaI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI XbaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AatII 2781 GACGTC GACGT^C
Acc65I 4768 GGTACC G^GTACC
AccIII 4115 TCCGGA T^CCGGA
AflIII 4775 ACATGT A^C[GA][CT]GT
AscI 655 GGCGCGCC GG^CGCGCC
Asp718I 4768 GGTACC G^GTACC
AspI 764 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2135 TGCGCA TGC^GCA
AvrII 841 CCTAGG C^CTAGG
BalI 2810 TGGCCA TGG^CCA
BamHI 4609 GGATCC G^GATCC
BbrPI 3532 CACGTG CAC^GTG
BclI 3859 TGATCA T^GATCA
BlnI 841 CCTAGG C^CTAGG
BsaAI 3532 CACGTG [CT]AC^GT[GA]
BsaBI 5135 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 4115 TCCGGA T^CCGGA
BsmBI 216 CGTCTC CGTCTC(1/5)
BsmI 4084 GAATGC GAATGC(1/-1)
BspEI 4115 TCCGGA T^CCGGA
BspHI 3688 TCATGA T^CATGA
BspLU11I 4775 ACATGT A^CATGT
BstEII 1440 GGTCACC G^GT[ACGT]ACC
BstPI 1440 GGTCACC G^GT[ACGT]ACC
BstXI 2913 CCACACAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 2824 GACGCCAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1440 GGTCACC G^GT[ACGT]ACC
EcoRI 1195 GAATTC G^AATTC
FbaI 3859 TGATCA T^GATCA
FseI 819 GGCCGGCC GGCCGG^CC
FspI 2135 TGCGCA TGC^GCA
KpnI 4768 GGTACC GGTAC^C
KspI 907 CCGCGG CCGC^GG
MamI 5135 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 4013 CAATTG C^AATTG
MluNI 2810 TGGCCA TGG^CCA
MroI 4115 TCCGGA T^CCGGA
MscI 2810 TGGCCA TGG^CCA
MunI 4013 CAATTG C^AATTG
NcoI 4903 CCATGG C^CATGG
NspI 4775 ACATGT [GA]CATG^[CT]
PflFI 764 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3532 CACGTG CAC^GTG
PmlI 3532 CACGTG CAC^GTG
PvuI 2299 CGATCG CGAT^CG
RcaI 3688 TCATGA T^CATGA
SacII 907 CCGCGG CCGC^GG
SapI 4355 GCTCTTC GCTCTTC(1/4)
SexAI 4057 ACCAGGT A^CC[AT]GGT
StuI 2361 AGGCCT AGG^CCT
SwaI 1152 ATTTAAAT ATTT^AAAT
Tth111I 764 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccI 2566 GTCTAC GT^[AC][GT]AC
4735 GTAGAC
AcsI 1156 AAATTC [GA]^AATT[CT]
1195 GAATTC
Alw44I 3811 GTGCAC G^TGCAC
4300 GTGCAC
Aor51HI 228 AGCGCT AGC^GCT
276 AGCGCT
ApaLI 3811 GTGCAC G^TGCAC
4300 GTGCAC
ApoI 1156 AAATTC [GA]^AATT[CT]
1195 GAATTC
BbsI 1260 GAAGAC GAAGAC(2/6)
4400 GAAGAC
BbvCI 4277 CCTCAGC CCTCAGC(-5/-2)
4978 GCTGAGG
BciVI 54 GTATCC GTATCC(6/5)
1104 GTATCC
BglI 1963 GCCTCGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2609 GCCAGCCAGGC
BglII 2594 AGATCT A^GATCT
3047 AGATCT
BlpI 272 GCTGAGC GC^T[ACGT]AGC
5038 GCTGAGC
Bpu1102I 272 GCTGAGC GC^T[ACGT]AGC
5038 GCTGAGC
BpuAI 1260 GAAGAC GAAGAC(2/6)
4400 GAAGAC
BsrDI 2862 CATTGC GCAATG(2/0)
4324 GCAATG
CelII 272 GCTGAGC GC^T[ACGT]AGC
5038 GCTGAGC
DraIII 4681 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
5077 CACCCTGTG
Eco47III 228 AGCGCT AGC^GCT
276 AGCGCT
PshAI 2008 GACCTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2889 GACCCTGGTC
PstI 2279 CTGCAG CTGCA^G
2847 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BanI BanII BbeI BbvI
BcgI BcnI BfaI BmyI BpmI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssHII BssKI
BssSI BstAPI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EagI EarI EclXI Eco52I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NdeII NgoMIV NlaIII
NlaIV PflMI PleI PpuMI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmaI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmaCI XmaI