##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AgeI Aor51HI ApaI AscI
Asp718I AvrII BalI BbeI BbrPI
BcgI BlnI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI BspEI BsrBI
BssHII BssSI Bst1107I BstUI BstZ17I
CpoI DraIII EagI EclXI Eco47III
Eco52I FseI HgaI Hin1I HindIII
KasI KpnI KspI MfeI MluI
MluNI MroI MscI MunI MvnI
NarI NdeI NheI NotI NruI
PacI PflMI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SacII SalI SbfI SexAI SfiI
SfoI SgrAI SmaI SnaBI Sse8387I
StuI SwaI Van91I XcmI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccI 3926 GTCTAC GT^[AC][GT]AC
AflII 3000 CTTAAG C^TTAAG
AhdI 5252 GACCTGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 3331 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 5361 GAAACTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 5252 GACCTGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3620 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 4754 CTCGAG C^[CT]CG[GA]G
AviII 3848 TGCGCA TGC^GCA
BamHI 1683 GGATCC G^GATCC
BciVI 3647 GGATAC GTATCC(6/5)
BfrI 3000 CTTAAG C^TTAAG
BglI 411 GCCTTCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 1665 CTCCAG CTGGAG(16/14)
BsoBI 4754 CTCGAG C^[CT]CG[GA]G
BspHI 1104 TCATGA T^CATGA
BsrFI 3990 GCCGGC [GA]^CCGG[CT]
BstAPI 4148 GCAGATAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2902 TTCGAA TT^CGAA
BstEII 3490 GGTTACC G^GT[ACGT]ACC
BstPI 3490 GGTTACC G^GT[ACGT]ACC
Cfr10I 3990 GCCGGC [GA]^CCGG[CT]
DrdI 3632 GACCTTTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 2930 CCATGG C^C[GA][CT]GG
EaeI 242 TGGCCG [CT]^GGCC[GA]
Eam1105I 5252 GACCTGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 4982 CCTCAGATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 3490 GGTTACC G^GT[ACGT]ACC
EcoRI 2732 GAATTC G^AATTC
EcoRV 4538 GATATC GAT^ATC
FspI 3848 TGCGCA TGC^GCA
HaeII 1543 AGCGCC [GA]GCGC^[CT]
NaeI 3990 GCCGGC GCC^GGC
NcoI 2930 CCATGG C^CATGG
NgoMIV 3990 GCCGGC G^CCGGC
NspV 2902 TTCGAA TT^CGAA
PaeR7I 4754 CTCGAG C^TCGAG
PflFI 3620 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 4978 AGGTCCT [GA]G^G[AT]CC[CT]
RcaI 1104 TCATGA T^CATGA
SapI 3868 GCTCTTC GCTCTTC(1/4)
ScaI 2511 AGTACT AGT^ACT
SfuI 2902 TTCGAA TT^CGAA
SpeI 3178 ACTAGT A^CTAGT
SphI 362 GCATGC GCATG^C
Tth111I 3620 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 4754 CTCGAG C^TCGAG
XmnI 5361 GAAACTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
Alw44I 4110 GTGCAC G^TGCAC
5723 GTGCAC
AocI 1397 CCTCAGG CC^T[ACGT]AGG
2432 CCTCAGG
ApaLI 4110 GTGCAC G^TGCAC
5723 GTGCAC
AseI 2113 ATTAAT AT^TAAT
4304 ATTAAT
AsnI 2113 ATTAAT AT^TAAT
4304 ATTAAT
BanI 1448 GGTGCC G^G[CT][GA]CC
2363 GGCACC
BglII 674 AGATCT A^GATCT
2754 AGATCT
BlpI 3078 GCTTAGC GC^T[ACGT]AGC
4657 GCTAAGC
Bpu1102I 3078 GCTTAGC GC^T[ACGT]AGC
4657 GCTAAGC
BsaAI 1750 CACGTA [CT]AC^GT[GA]
2372 CACGTA
BspDI 441 ATCGAT AT^CGAT
1016 ATCGAT
BspLU11I 4329 ACATGT A^CATGT
5056 ACATGT
Bsu36I 1397 CCTCAGG CC^T[ACGT]AGG
2432 CCTCAGG
CelII 3078 GCTTAGC GC^T[ACGT]AGC
4657 GCTAAGC
ClaI 441 ATCGAT AT^CGAT
1016 ATCGAT
Eco81I 1397 CCTCAGG CC^T[ACGT]AGG
2432 CCTCAGG
EcoT22I 140 ATGCAT ATGCA^T
775 ATGCAT
HpaI 1022 GTTAAC GTT^AAC
5443 GTTAAC
NsiI 140 ATGCAT ATGCA^T
775 ATGCAT
PshBI 2113 ATTAAT AT^TAAT
4304 ATTAAT
PstI 4995 CTGCAG CTGCA^G
5265 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AspHI AvaII BanII
BbsI BbvCI BbvI BclI BcnI
BfaI BmyI BpuAI BsaBI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
Bsp1407I BspMI BsrDI BsrGI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII EarI EcoO109I EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MamI
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XbaI XhoII