##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
AatII AflII Alw44I AocI ApaLI
AscI AseI AsnI AspI AviII
AvrII BbeI BfrI BlnI Bsp1407I
BspDI BspLU11I BsrGI Bst1107I BstBI
BstZ17I Bsu36I ClaI CpoI Eco81I
EcoT22I FseI FspI HpaI KasI
NarI NotI NruI NsiI NspI
NspV PacI PflFI PmeI PshBI
PvuI RsrII SbfI SfoI SfuI
SmaI SnaBI SphI Sse8387I SspBI
SspI SwaI Tth111I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
Acc65I 5236 GGTACC G^GTACC
AccIII 3666 TCCGGA T^CCGGA
AgeI 2455 ACCGGT A^CCGGT
Aor51HI 55 AGCGCT AGC^GCT
Asp718I 5236 GGTACC G^GTACC
BseAI 3666 TCCGGA T^CCGGA
BsiWI 5357 CGTACG C^GTACG
BspEI 3666 TCCGGA T^CCGGA
BsrBI 4134 GAGCGG CCGCTC(-3/-3)
BssHII 4404 GCGCGC G^CGCGC
DrdI 4042 GACTCTACGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1368 CGGCCG C^GGCCG
EclXI 1368 CGGCCG C^GGCCG
Eco47III 55 AGCGCT AGC^GCT
Eco52I 1368 CGGCCG C^GGCCG
EcoRI 3617 GAATTC G^AATTC
EcoRV 2635 GATATC GAT^ATC
KpnI 5236 GGTACC GGTAC^C
KspI 4416 CCGCGG CCGC^GG
MfeI 5788 CAATTG C^AATTG
MluI 2315 ACGCGT A^CGCGT
MroI 3666 TCCGGA T^CCGGA
MunI 5788 CAATTG C^AATTG
NheI 4225 GCTAGC G^CTAGC
PaeR7I 1972 CTCGAG C^TCGAG
PinAI 2455 ACCGGT A^CCGGT
PshAI 2163 GACCACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 4416 CCGCGG CCGC^GG
SfiI 5342 GGCCAGGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 2454 CACCGGTG C[GA]^CCGG[CT]G
SpeI 6136 ACTAGT A^CTAGT
XhoI 1972 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AclI 1613 AACGTT AA^CGTT
4580 AACGTT
AhdI 1256 GACCTATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4444 GACGACCTGTC
AspEI 1256 GACCTATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4444 GACGACCTGTC
BamHI 2332 GGATCC G^GATCC
4725 GGATCC
BbrPI 2254 CACGTG CAC^GTG
4195 CACGTG
BclI 2306 TGATCA T^GATCA
2660 TGATCA
BglII 3068 AGATCT A^GATCT
6388 AGATCT
BsaI 1544 GAGACC GGTCTC(1/5)
711 GGTCTC
BsiEI 1368 CGGCCG CG[GA][CT]^CG
4308 CGACCG
BspHI 3906 TCATGA T^CATGA
5765 TCATGA
BstAPI 841 GCAGCCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2086 GCAGTCACTGC
BstEII 3610 GGTTACC G^GT[ACGT]ACC
5470 GGTTACC
BstPI 3610 GGTTACC G^GT[ACGT]ACC
5470 GGTTACC
Eam1105I 1256 GACCTATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4444 GACGACCTGTC
EcoO65I 3610 GGTTACC G^GT[ACGT]ACC
5470 GGTTACC
FbaI 2306 TGATCA T^GATCA
2660 TGATCA
NaeI 1383 GCCGGC GCC^GGC
4229 GCCGGC
NdeI 455 CATATG CA^TATG
2624 CATATG
NgoMIV 1383 GCCGGC G^CCGGC
4229 GCCGGC
PmaCI 2254 CACGTG CAC^GTG
4195 CACGTG
PmlI 2254 CACGTG CAC^GTG
4195 CACGTG
Psp1406I 1613 AACGTT AA^CGTT
4580 AACGTT
RcaI 3906 TCATGA T^CATGA
5765 TCATGA
SalI 2590 GTCGAC G^TCGAC
3643 GTCGAC
SapI 2681 GAAGAGC GCTCTTC(1/4)
4563 GAAGAGC
ScaI 2721 AGTACT AGT^ACT
2937 AGTACT
SexAI 4607 ACCAGGT A^CC[AT]GGT
5088 ACCAGGT
StuI 3003 AGGCCT AGG^CCT
5153 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 143 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
ApaI ApoI Asp700I AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcgI BciVI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrDI
BsrFI BsrI BssKI BssSI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DraIII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV PflMI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XbaI
XcmI XhoII XmnI