##################################
# NO CUT ENZYMES ( 35 enzymes) #
##################################
AclI AgeI AscI AviII BsiWI
BspDI BssHII ClaI CpoI EagI
EclXI Eco52I EcoRV FseI FspI
HpaI KspI MluI NaeI NgoMIV
NotI PacI PinAI PmeI Psp1406I
PvuI RsrII SacII SalI SbfI
SfiI SgrAI SnaBI SpeI Sse8387I
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AatII 2 GACGTC GACGT^C
AccIII 1605 TCCGGA T^CCGGA
Aor51HI 4505 AGCGCT AGC^GCT
AseI 11407 ATTAAT AT^TAAT
AsnI 11407 ATTAAT AT^TAAT
AvrII 4817 CCTAGG C^CTAGG
BbeI 1997 GGCGCC GGCGC^C
BcgI 634 GCATGACCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 4817 CCTAGG C^CTAGG
BseAI 1605 TCCGGA T^CCGGA
BsiEI 2849 CGACCG CG[GA][CT]^CG
BspEI 1605 TCCGGA T^CCGGA
Bst1107I 11414 GTATAC GTA^TAC
BstBI 9313 TTCGAA TT^CGAA
BstEII 7550 GGTCACC G^GT[ACGT]ACC
BstPI 7550 GGTCACC G^GT[ACGT]ACC
BstZ17I 11414 GTATAC GTA^TAC
Eco47III 4505 AGCGCT AGC^GCT
EcoO65I 7550 GGTCACC G^GT[ACGT]ACC
EcoRI 1524 GAATTC G^AATTC
KasI 1997 GGCGCC G^GCGCC
MroI 1605 TCCGGA T^CCGGA
NarI 1997 GGCGCC GG^CGCC
NdeI 16 CATATG CA^TATG
NheI 10620 GCTAGC G^CTAGC
NruI 1367 TCGCGA TCG^CGA
NspV 9313 TTCGAA TT^CGAA
PaeR7I 7527 CTCGAG C^TCGAG
PshAI 2 GACGTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 11407 ATTAAT AT^TAAT
ScaI 2365 AGTACT AGT^ACT
SfoI 1997 GGCGCC GGC^GCC
SfuI 9313 TTCGAA TT^CGAA
SwaI 11349 ATTTAAAT ATTT^AAAT
XbaI 4445 TCTAGA T^CTAGA
XhoI 7527 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
BamHI 8610 GGATCC G^GATCC
9927 GGATCC
BbrPI 7304 CACGTG CAC^GTG
10527 CACGTG
BglI 5835 GCCAGGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
8552 GCCTTCCTGGC
BsaAI 7304 CACGTG [CT]AC^GT[GA]
10527 CACGTG
Bsp1407I 2383 TGTACA T^GTACA
6220 TGTACA
BsrGI 2383 TGTACA T^GTACA
6220 TGTACA
EcoT22I 920 ATGCAT ATGCA^T
6550 ATGCAT
NsiI 920 ATGCAT ATGCA^T
6550 ATGCAT
PmaCI 7304 CACGTG CAC^GTG
10527 CACGTG
PmlI 7304 CACGTG CAC^GTG
10527 CACGTG
SphI 1481 GCATGC GCATG^C
9718 GCATGC
SspBI 2383 TGTACA T^GTACA
6220 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 175 enzymes) #
#########################################
Acc65I AccI AccII AciI AcsI
AcyI AfaI AflII AflIII AhdI
AluI Alw44I AlwI AlwNI AocI
ApaI ApaLI ApoI Asp700I Asp718I
AspEI AspHI AspI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BciVI BclI BcnI BfaI
BfrI BglII BlpI BmyI BpmI
Bpu1102I BpuAI BsaBI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsmI BsoBI Bsp1286I BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DraIII DrdI
DsaI EaeI Eam1105I EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HinfI HpaII
HphI ItaI KpnI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MfeI MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MunI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflFI
PflMI PleI PpuMI PstI PvuII
RcaI RsaI SacI SapI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SmaI SmlI SspI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XcmI XhoII XmaCI XmaI XmnI