##################################
# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI Aor51HI ApaI
AscI AseI AsnI Asp718I AvaI
AviII AvrII BalI BamHI BbeI
BcnI BfrI BglI BlnI BlpI
Bpu1102I BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BsoBI
BspDI BspEI BsrBI BsrFI BssHII
Bst1107I BstBI BstUI BstZ17I CelII
CfoI Cfr10I ClaI CpoI DraI
DraII DraIII EagI EclXI Eco47III
Eco52I EcoNI EcoO109I FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HpaI HpaII KasI KpnI
KspI MluI MluNI MroI MscI
MspI MvnI NaeI NarI NciI
NdeI NgoMIV NotI NruI NspV
PacI PaeR7I PflMI PinAI PmeI
PpuMI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI StuI SwaI Van91I
XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AccI 2885 GTAGAC GT^[AC][GT]AC
AhdI 2660 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2386 GTGCAC G^TGCAC
AocI 2567 CCTCAGG CC^T[ACGT]AGG
ApaLI 2386 GTGCAC G^TGCAC
Asp700I 2849 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2660 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2967 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanII 2915 GAGCCC G[GA]GC[CT]^C
BbrPI 2715 CACGTG CAC^GTG
BbvCI 285 CCTCAGC CCTCAGC(-5/-2)
BcgI 1719 CGATGGTAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaBI 1304 GATGCTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1407I 2380 TGTACA T^GTACA
BspHI 1060 TCATGA T^CATGA
BspLU11I 3478 ACATGT A^CATGT
BsrDI 225 CATTGC GCAATG(2/0)
BsrGI 2380 TGTACA T^GTACA
BssKI 1108 CCAGG ^CC[ACGT]GG
BssSI 3401 CACGAG CACGAG(-5/-1)
BstNI 1108 CCAGG CC^[AT]GG
BstXI 1363 CCAAATGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2567 CCTCAGG CC^T[ACGT]AGG
DrdI 1708 GACAGCTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1040 CGGCCA [CT]^GGCC[GA]
Eam1105I 2660 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2567 CCTCAGG CC^T[ACGT]AGG
EcoRII 1108 CCAGG ^CC[AT]GG
EcoRV 3008 GATATC GAT^ATC
EcoT22I 2580 ATGCAT ATGCA^T
FauI 3733 CCCGC CCCGC(4/6)
HincII 2029 GTCAAC GT[CT]^[GA]AC
HindII 2029 GTCAAC GT[CT]^[GA]AC
MamI 1304 GATGCTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvaI 1108 CCAGG CC^[AT]GG
NheI 1519 GCTAGC G^CTAGC
NsiI 2580 ATGCAT ATGCA^T
PflFI 2967 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2715 CACGTG CAC^GTG
PmlI 2715 CACGTG CAC^GTG
RcaI 1060 TCATGA T^CATGA
SapI 2912 GAAGAGC GCTCTTC(1/4)
SbfI 2947 CCTGCAGG CCTGCA^GG
ScrFI 1108 CCAGG CC^[ACGT]GG
SmlI 3684 CTCAAG C^T[CT][GA]AG
SphI 2500 GCATGC GCATG^C
Sse8387I 2947 CCTGCAGG CCTGCA^GG
SspBI 2380 TGTACA T^GTACA
TaqI 1718 TCGA T^CGA
Tth111I 2967 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1718 TCGA T^CGA
XmnI 2849 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AciI 3734 CCGC CCGC(-3/-1)
1039 GCGG
AflIII 2714 ACACGT A^C[GA][CT]GT
3478 ACATGT
AlwI 7 GATCC GGATC(4/5)
2606 GATCC
AlwNI 1498 CAGTAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1557 CAGATACTG
AspHI 2386 GTGCAC G[AT]GC[AT]^C
2533 GTGCTC
BanI 2638 GGCACC G^G[CT][GA]CC
2953 GGCACC
BbsI 1288 GAAGAC GAAGAC(2/6)
1741 GTCTTC
BciVI 3133 GGATAC GTATCC(6/5)
1867 GTATCC
BclI 2413 TGATCA T^GATCA
2869 TGATCA
BglII 566 AGATCT A^GATCT
757 AGATCT
BpuAI 1288 GAAGAC GAAGAC(2/6)
1741 GTCTTC
BsiHKAI 2386 GTGCAC G[AT]GC[AT]^C
2533 GTGCTC
BsmAI 901 GAGAC GTCTC(1/5)
832 GTCTC
BstAPI 1916 GCAGAAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1931 GCAACTGCTGC
BstEII 1373 GGTAACC G^GT[ACGT]ACC
3406 GGTAACC
BstPI 1373 GGTAACC G^GT[ACGT]ACC
3406 GGTAACC
DsaI 1509 CCATGG C^C[GA][CT]GG
3039 CCATGG
EcoO65I 1373 GGTAACC G^GT[ACGT]ACC
3406 GGTAACC
EcoRI 820 GAATTC G^AATTC
958 GAATTC
FbaI 2413 TGATCA T^GATCA
2869 TGATCA
HaeIII 1041 GGCC GG^CC
2732 GGCC
NcoI 1509 CCATGG C^CATGG
3039 CCATGG
NspI 2500 GCATGC [GA]CATG^[CT]
3478 ACATGT
XbaI 2479 TCTAGA T^CTAGA
3786 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AcsI AfaI AluI ApoI AvaII
BbvI BfaI BmyI BpmI BsaAI
BsaJI BseRI BsiYI BslI BsmFI
BsmI Bsp1286I BspMI BsrI BstF5I
BstYI Cac8I Cfr13I DdeI DpnII
EarI EcoT14I Fnu4HI FokI HindIII
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MfeI
MflI MnlI MseI MslI MspA1I
MunI MwoI NdeII NlaIII NlaIV
PleI PstI PvuII RsaI Sau3AI
Sau96I ScaI SfaNI SfcI SspI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII