##################################
# NO CUT ENZYMES ( 46 enzymes) #
##################################
AccIII AgeI AhdI Aor51HI AscI
AseI AsnI AspEI AspI AviII
BciVI BclI BseAI BsiWI BsmBI
BspEI BssHII Eam1105I Eco47III EcoRV
FbaI FseI FspI HpaI MfeI
MluI MroI MunI NheI NotI
NruI PacI PflFI PinAI PmeI
PshBI PvuI SalI SbfI SexAI
SgrAI Sse8387I SspI SwaI Tth111I
XbaI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AatII 6347 GACGTC GACGT^C
Acc65I 7184 GGTACC G^GTACC
AccI 83 GTATAC GT^[AC][GT]AC
AclI 4007 AACGTT AA^CGTT
AflII 2116 CTTAAG C^TTAAG
Alw44I 8019 GTGCAC G^TGCAC
ApaLI 8019 GTGCAC G^TGCAC
Asp700I 7534 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 7184 GGTACC G^GTACC
AvrII 394 CCTAGG C^CTAGG
BfrI 2116 CTTAAG C^TTAAG
BlnI 394 CCTAGG C^CTAGG
BsaWI 3359 TCCGGT [AT]^CCGG[AT]
BspDI 2138 ATCGAT AT^CGAT
Bst1107I 83 GTATAC GTA^TAC
BstBI 6212 TTCGAA TT^CGAA
BstZ17I 83 GTATAC GTA^TAC
ClaI 2138 ATCGAT AT^CGAT
CpoI 2297 CGGACCG CG^G[AT]CCG
DraIII 4296 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 618 GACACAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HindIII 5601 AAGCTT A^AGCTT
KpnI 7184 GGTACC GGTAC^C
KspI 6259 CCGCGG CCGC^GG
NspV 6212 TTCGAA TT^CGAA
PshAI 6915 GACCGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 4007 AACGTT AA^CGTT
RsrII 2297 CGGACCG CG^G[AT]CCG
SacII 6259 CCGCGG CCGC^GG
SfiI 7714 GGCCCGGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 6212 TTCGAA TT^CGAA
SnaBI 116 TACGTA TAC^GTA
SpeI 1877 ACTAGT A^CTAGT
XmnI 7534 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
BbeI 390 GGCGCC GGCGC^C
6655 GGCGCC
BbrPI 2747 CACGTG CAC^GTG
7055 CACGTG
BglII 5034 AGATCT A^GATCT
6115 AGATCT
BsaBI 6202 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
7594 GATTCCCATC
BsmI 429 GAATGC GAATGC(1/-1)
1322 GCATTC
BspLU11I 3936 ACATGT A^CATGT
7162 ACATGT
BsrDI 3822 GCAATG GCAATG(2/0)
5012 GCAATG
BstEII 1892 GGTCACC G^GT[ACGT]ACC
5041 GGTCACC
BstPI 1892 GGTCACC G^GT[ACGT]ACC
5041 GGTCACC
EcoO65I 1892 GGTCACC G^GT[ACGT]ACC
5041 GGTCACC
EcoRI 4496 GAATTC G^AATTC
6294 GAATTC
EcoT22I 97 ATGCAT ATGCA^T
3110 ATGCAT
KasI 390 GGCGCC G^GCGCC
6655 GGCGCC
MamI 6202 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
7594 GATTCCCATC
NarI 390 GGCGCC GG^CGCC
6655 GGCGCC
NdeI 74 CATATG CA^TATG
2953 CATATG
NsiI 97 ATGCAT ATGCA^T
3110 ATGCAT
PmaCI 2747 CACGTG CAC^GTG
7055 CACGTG
PmlI 2747 CACGTG CAC^GTG
7055 CACGTG
ScaI 1599 AGTACT AGT^ACT
2177 AGTACT
SfoI 390 GGCGCC GGC^GCC
6655 GGCGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 157 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApaI ApoI AspHI AvaI AvaII
BalI BamHI BanI BanII BbsI
BbvCI BbvI BcgI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaHI BsaI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
Bsp1407I BspHI BspMI BsrBI BsrFI
BsrGI BsrI BssKI BssSI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EagI EarI EclXI
Eco52I Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PaeR7I PflMI PleI
PpuMI PstI PvuII RcaI RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI SphI
SspBI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoI XhoII
XmaCI XmaI