##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
AhdI Aor51HI AscI Asp718I AspEI
AviII AvrII BcgI BclI BfrI
BlnI BsaWI BseAI BsiEI BsiWI
BspDI BspEI BsrBI BsrFI BssHII
BstBI BstEII BstPI Cfr10I ClaI
CpoI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I FbaI FseI FspI
HindIII KpnI KspI MfeI MluI
MroI MunI NaeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PinAI PmeI Psp1406I PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI SfiI SfuI SgrAI SnaBI
SpeI Sse8387I SwaI XhoI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
Alw44I 1620 GTGCAC G^TGCAC
ApaI 1637 GGGCCC GGGCC^C
ApaLI 1620 GTGCAC G^TGCAC
AseI 1511 ATTAAT AT^TAAT
AsnI 1511 ATTAAT AT^TAAT
AspI 1683 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1223 GGATCC G^GATCC
BbeI 2768 GGCGCC GGCGC^C
BbrPI 4676 CACGTG CAC^GTG
BbvCI 1560 GCTGAGG CCTCAGC(-5/-2)
BglI 1084 GCCCACCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 2618 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2618 GCTCAGC GC^T[ACGT]AGC
BsaBI 4136 GATGCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 2100 GCATTC GAATGC(1/-1)
BspHI 1163 TCATGA T^CATGA
BspLU11I 1345 ACATGT A^CATGT
BspMI 1767 ACCTGC ACCTGC(4/8)
BssSI 3816 CTCGTG CACGAG(-5/-1)
Bst1107I 1358 GTATAC GTA^TAC
BstAPI 752 GCAAGATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 1358 GTATAC GTA^TAC
CelII 2618 GCTCAGC GC^T[ACGT]AGC
DrdI 4386 GACAGTGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 4489 GAAGAG CTCTTC(1/4)
EcoRI 3292 GAATTC G^AATTC
EcoRV 1265 GATATC GAT^ATC
EcoT22I 844 ATGCAT ATGCA^T
HaeII 2768 GGCGCC [GA]GCGC^[CT]
HgaI 2727 GACGC GACGC(5/10)
HincII 3726 GTTAAC GT[CT]^[GA]AC
HindII 3726 GTTAAC GT[CT]^[GA]AC
HpaI 3726 GTTAAC GTT^AAC
KasI 2768 GGCGCC G^GCGCC
Ksp632I 4489 GAAGAG CTCTTC(1/4)
MamI 4136 GATGCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 2922 CAGCTG C[AC]G^C[GT]G
NarI 2768 GGCGCC GG^CGCC
NsiI 844 ATGCAT ATGCA^T
PflFI 1683 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 4651 CCAGGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 4676 CACGTG CAC^GTG
PmlI 4676 CACGTG CAC^GTG
PshAI 2648 GACTGCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1511 ATTAAT AT^TAAT
PvuII 2922 CAGCTG CAG^CTG
RcaI 1163 TCATGA T^CATGA
SfoI 2768 GGCGCC GGC^GCC
SmaI 4470 CCCGGG CCC^GGG
SphI 3896 GCATGC GCATG^C
StuI 2775 AGGCCT AGG^CCT
Tth111I 1683 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 4651 CCAGGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 3416 CCAGTTTTTGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 4470 CCCGGG C^CCGGG
XmaI 4470 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AatII 1877 GACGTC GACGT^C
2861 GACGTC
AccII 10 CGCG CG^CG
2765 CGCG
AlwNI 2161 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
2644 CAGAGACTG
AocI 1723 CCTTAGG CC^T[ACGT]AGG
4554 CCTCAGG
BciVI 3994 GGATAC GTATCC(6/5)
443 GTATCC
BsaAI 4176 CACGTA [CT]AC^GT[GA]
4676 CACGTG
BsaI 883 GAGACC GGTCTC(1/5)
4617 GGTCTC
BsmBI 1704 GAGACG CGTCTC(1/5)
2364 GAGACG
Bsp1407I 3009 TGTACA T^GTACA
3279 TGTACA
BsrDI 4292 CATTGC GCAATG(2/0)
1942 GCAATG
BsrGI 3009 TGTACA T^GTACA
3279 TGTACA
BstUI 10 CGCG CG^CG
2765 CGCG
BstXI 2253 CCACCCAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4382 CCAGGACAGTGG
Bsu36I 1723 CCTTAGG CC^T[ACGT]AGG
4554 CCTCAGG
DraIII 856 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
2821 CACCAGGTG
Eco81I 1723 CCTTAGG CC^T[ACGT]AGG
4554 CCTCAGG
EcoNI 4408 CCTGGGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4757 CCTACCCAAGG
FauI 17 CCCGC CCCGC(4/6)
38 GCGGG
HapII 1244 CCGG C^CGG
4471 CCGG
HpaII 1244 CCGG C^CGG
4471 CCGG
MslI 837 CATTCAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2831 CACTTCAGTG
MspI 1244 CCGG C^CGG
4471 CCGG
MvnI 10 CGCG CG^CG
2765 CGCG
NdeI 3538 CATATG CA^TATG
4297 CATATG
PpuMI 1306 GGGACCT [GA]G^G[AT]CC[CT]
4429 GGGTCCC
PstI 2749 CTGCAG CTGCA^G
2886 CTGCAG
SspBI 3009 TGTACA T^GTACA
3279 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AflIII AluI AlwI ApoI Asp700I
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvI BcnI BfaI
BglII BmyI BpmI BpuAI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HhaI Hin1I HinP1I HinfI HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PleI
RsaI Sau3AI Sau96I ScrFI SexAI
SfaNI SfcI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XbaI XhoII
XmnI