##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII Aor51HI ApaI AscI Asp718I
AvaI AviII BbeI BbrPI BclI
BfrI BlpI Bpu1102I BsaHI BseAI
BseRI BsiEI BsiWI BsmBI BsoBI
BspDI BspEI BsrBI BsrDI BssHII
BstBI CelII ClaI CpoI DrdI
EagI EclXI Eco47III Eco52I EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HgaI Hin1I KasI KpnI
KspI MfeI MluI MroI MunI
NaeI NarI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
SfiI SfoI SfuI SmaI SnaBI
Sse8387I SwaI XcmI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AccII 5616 CGCG CG^CG
AflIII 4996 ACATGT A^C[GA][CT]GT
AgeI 760 ACCGGT A^CCGGT
AhdI 3284 GACAGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 728 GTGCAC G^TGCAC
AocI 5444 CCTGAGG CC^T[ACGT]AGG
ApaLI 728 GTGCAC G^TGCAC
AspEI 3284 GACAGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3889 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3034 CCTAGG C^CTAGG
BanI 4577 GGCACC G^G[CT][GA]CC
BcgI 900 GCAACTGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 3821 GCCCAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2714 AGATCT A^GATCT
BlnI 3034 CCTAGG C^CTAGG
BsaAI 2702 CACGTA [CT]AC^GT[GA]
BsaBI 2217 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 760 ACCGGT [AT]^CCGG[AT]
Bsp1407I 979 TGTACA T^GTACA
BspHI 2867 TCATGA T^CATGA
BspLU11I 4996 ACATGT A^CATGT
BspMI 5432 GCAGGT ACCTGC(4/8)
BsrFI 760 ACCGGT [GA]^CCGG[CT]
BsrGI 979 TGTACA T^GTACA
BssSI 1265 CTCGTG CACGAG(-5/-1)
BstAPI 3830 GCAGAGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 5616 CGCG CG^CG
Bsu36I 5444 CCTGAGG CC^T[ACGT]AGG
CfoI 5617 GCGC GCG^C
Cfr10I 760 ACCGGT [GA]^CCGG[CT]
DraIII 2577 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 3284 GACAGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1372 GAAGAG CTCTTC(1/4)
Eco81I 5444 CCTGAGG CC^T[ACGT]AGG
EcoNI 3632 CCTGGCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HhaI 5617 GCGC GCG^C
HinP1I 5617 GCGC G^CGC
HindIII 1972 AAGCTT A^AGCTT
HpaI 3721 GTTAAC GTT^AAC
Ksp632I 1372 GAAGAG CTCTTC(1/4)
MamI 2217 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 2893 CAGCTG C[AC]G^C[GT]G
MvnI 5616 CGCG CG^CG
NdeI 4554 CATATG CA^TATG
NheI 2398 GCTAGC G^CTAGC
PflFI 3889 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 760 ACCGGT A^CCGGT
PstI 2317 CTGCAG CTGCA^G
PvuII 2893 CAGCTG CAG^CTG
RcaI 2867 TCATGA T^CATGA
SacI 12 GAGCTC GAGCT^C
ScaI 2016 AGTACT AGT^ACT
SgrAI 759 CACCGGTG C[GA]^CCGG[CT]G
SspBI 979 TGTACA T^GTACA
Tth111I 3889 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3955 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AciI 5389 GCGG CCGC(-3/-1)
5592 GCGG
AlwNI 898 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
2320 CAGCTCCTG
BbvCI 2183 GCTGAGG CCTCAGC(-5/-2)
3798 GCTGAGG
BciVI 5234 GGATAC GTATCC(6/5)
4277 GTATCC
BcnI 3509 CCCGG CC^[GC]GG
3766 CCGGG
Bst1107I 3119 GTATAC GTA^TAC
4394 GTATAC
BstEII 3238 GGTAACC G^GT[ACGT]ACC
3596 GGTCACC
BstPI 3238 GGTAACC G^GT[ACGT]ACC
3596 GGTCACC
BstZ17I 3119 GTATAC GTA^TAC
4394 GTATAC
EcoO65I 3238 GGTAACC G^GT[ACGT]ACC
3596 GGTCACC
HincII 656 GTCAAC GT[CT]^[GA]AC
3721 GTTAAC
HindII 656 GTCAAC GT[CT]^[GA]AC
3721 GTTAAC
MaeII 2703 ACGT A^CGT
4171 ACGT
NciI 3509 CCCGG CC^[GC]GG
3766 CCGGG
PflMI 2576 CCACACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3986 CCATTTTCTGG
PpuMI 228 AGGACCT [GA]G^G[AT]CC[CT]
2135 GGGTCCT
SexAI 3716 ACCTGGT A^CC[AT]GGT
4754 ACCTGGT
SpeI 1239 ACTAGT A^CTAGT
2883 ACTAGT
SphI 2370 GCATGC GCATG^C
5531 GCATGC
TaqI 741 TCGA T^CGA
909 TCGA
TthHB8I 741 TCGA T^CGA
909 TCGA
Van91I 2576 CCACACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3986 CCATTTTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AccI AcsI AfaI AluI AlwI
ApoI AseI AsnI Asp700I AspHI
AvaII BalI BamHI BanII BbsI
BbvI BfaI BmyI BpmI BpuAI
BsaI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EcoO109I
EcoRI EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI MaeI MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspI MvaI MwoI NcoI
NdeII NlaIII NlaIV NspI PleI
PshBI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StuI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII XmnI