##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AccIII AclI Alw44I Aor51HI ApaLI
AscI AseI AsnI AspI AvaI
AviII BbrPI BseAI BsiEI BsiWI
BsmBI BsoBI BspEI BspHI BsrBI
BssHII Bst1107I BstBI BstZ17I CpoI
EagI EclXI Eco47III Eco52I FseI
FspI HpaI KspI MfeI MroI
MunI NaeI NdeI NgoMIV NotI
NspV PacI PaeR7I PflFI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SbfI
SexAI SfiI SfuI SgrAI SmaI
SnaBI Sse8387I SwaI Tth111I XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 3506 GACGTC GACGT^C
Acc65I 600 GGTACC G^GTACC
AgeI 603 ACCGGT A^CCGGT
AhdI 4052 GACCACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 3998 GGGCCC GGGCC^C
Asp718I 600 GGTACC G^GTACC
AspEI 4052 GACCACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 1713 CCTAGG C^CTAGG
BcgI 5474 CGATCCGTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1955 GGATAC GTATCC(6/5)
BclI 968 TGATCA T^GATCA
BcnI 2775 CCCGG CC^[GC]GG
BglI 3982 GCCAACTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 4819 AGATCT A^GATCT
BlnI 1713 CCTAGG C^CTAGG
BsaAI 4269 TACGTG [CT]AC^GT[GA]
BsaWI 603 ACCGGT [AT]^CCGG[AT]
Bsp1407I 725 TGTACA T^GTACA
BspDI 5932 ATCGAT AT^CGAT
BspLU11I 415 ACATGT A^CATGT
BsrFI 603 ACCGGT [GA]^CCGG[CT]
BsrGI 725 TGTACA T^GTACA
BssSI 2163 CACGAG CACGAG(-5/-1)
BstXI 2374 CCAGGAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 603 ACCGGT [GA]^CCGG[CT]
ClaI 5932 ATCGAT AT^CGAT
DrdI 399 GACAATGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 4052 GACCACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 5683 ATGCAT ATGCA^T
FauI 1226 CCCGC CCCGC(4/6)
FbaI 968 TGATCA T^GATCA
KpnI 600 GGTACC GGTAC^C
MluI 4 ACGCGT A^CGCGT
NciI 2775 CCCGG CC^[GC]GG
NcoI 4867 CCATGG C^CATGG
NheI 2292 GCTAGC G^CTAGC
NruI 4571 TCGCGA TCG^CGA
NsiI 5683 ATGCAT ATGCA^T
PinAI 603 ACCGGT A^CCGGT
PpuMI 1999 AGGACCC [GA]G^G[AT]CC[CT]
SacI 1375 GAGCTC GAGCT^C
SalI 2812 GTCGAC G^TCGAC
SapI 6141 GCTCTTC GCTCTTC(1/4)
ScaI 2054 AGTACT AGT^ACT
SpeI 6113 ACTAGT A^CTAGT
SspBI 725 TGTACA T^GTACA
XbaI 326 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AflIII 4 ACGCGT A^C[GA][CT]GT
415 ACATGT
AlwNI 744 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG
2673 CAGAAACTG
AocI 3012 CCTGAGG CC^T[ACGT]AGG
5861 CCTGAGG
BbeI 3979 GGCGCC GGCGC^C
4063 GGCGCC
BbvCI 1243 CCTCAGC CCTCAGC(-5/-2)
4735 CCTCAGC
BlpI 715 GCTTAGC GC^T[ACGT]AGC
2481 GCTAAGC
Bpu1102I 715 GCTTAGC GC^T[ACGT]AGC
2481 GCTAAGC
BsaI 618 GGTCTC GGTCTC(1/5)
1265 GGTCTC
BsrDI 491 CATTGC GCAATG(2/0)
3354 GCAATG
BstEII 1779 GGTTACC G^GT[ACGT]ACC
3647 GGTTACC
BstPI 1779 GGTTACC G^GT[ACGT]ACC
3647 GGTTACC
Bsu36I 3012 CCTGAGG CC^T[ACGT]AGG
5861 CCTGAGG
CelII 715 GCTTAGC GC^T[ACGT]AGC
2481 GCTAAGC
DraII 1999 AGGACCC [GA]G^G[ACGT]CC[CT]
3997 AGGGCCC
DraIII 2948 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
3169 CACGCAGTG
DsaI 4867 CCATGG C^C[GA][CT]GG
6166 CCGTGG
Eco81I 3012 CCTGAGG CC^T[ACGT]AGG
5861 CCTGAGG
EcoO109I 1999 AGGACCC [GA]G^G[ACGT]CC[CT]
3997 AGGGCCC
EcoO65I 1779 GGTTACC G^GT[ACGT]ACC
3647 GGTTACC
HaeII 3979 GGCGCC [GA]GCGC^[CT]
4063 GGCGCC
HincII 1843 GTCAAC GT[CT]^[GA]AC
2812 GTCGAC
HindII 1843 GTCAAC GT[CT]^[GA]AC
2812 GTCGAC
KasI 3979 GGCGCC G^GCGCC
4063 GGCGCC
MaeII 3507 ACGT A^CGT
4270 ACGT
NarI 3979 GGCGCC GG^CGCC
4063 GGCGCC
SfoI 3979 GGCGCC GGC^GCC
4063 GGCGCC
SphI 2855 GCATGC GCATG^C
5875 GCATGC
SspI 4035 AATATT AAT^ATT
4186 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflII AluI AlwI ApoI
Asp700I AspHI AvaII BalI BamHI
BanI BanII BbsI BbvI BfaI
BfrI BmyI BpmI BpuAI BsaBI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BspMI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
EaeI EarI EcoNI EcoRI EcoRII
EcoRV EcoT14I Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MamI MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NdeII NlaIII NlaIV
NspI PflMI PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII XmnI