##################################
# NO CUT ENZYMES ( 52 enzymes) #
##################################
AatII Acc65I AclI AgeI AscI
Asp718I AvrII BbeI BbrPI BbvCI
BcgI BlnI BsiEI BsiWI BspDI
BspLU11I BssHII BstBI ClaI CpoI
EagI EclXI Eco52I EcoT22I FseI
KasI KpnI MluI NarI NheI
NotI NruI NsiI NspV PacI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RsrII SalI SfiI
SfoI SfuI SgrAI SpeI SphI
StuI SwaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccIII 4208 TCCGGA T^CCGGA
AflIII 6887 ACACGT A^C[GA][CT]GT
AhdI 3993 GACCATTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 6989 GTGCAC G^TGCAC
Aor51HI 1649 AGCGCT AGC^GCT
ApaLI 6989 GTGCAC G^TGCAC
AseI 3199 ATTAAT AT^TAAT
AsnI 3199 ATTAAT AT^TAAT
AspEI 3993 GACCATTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2825 TGCGCA TGC^GCA
BclI 2321 TGATCA T^GATCA
BlpI 112 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 112 GCTCAGC GC^T[ACGT]AGC
BsaAI 1720 TACGTA [CT]AC^GT[GA]
BsaWI 4208 TCCGGA [AT]^CCGG[AT]
BseAI 4208 TCCGGA T^CCGGA
BsmBI 1272 GAGACG CGTCTC(1/5)
Bsp1407I 684 TGTACA T^GTACA
BspEI 4208 TCCGGA T^CCGGA
BspHI 2913 TCATGA T^CATGA
BsrGI 684 TGTACA T^GTACA
Bst1107I 5517 GTATAC GTA^TAC
BstEII 4740 GGTGACC G^GT[ACGT]ACC
BstPI 4740 GGTGACC G^GT[ACGT]ACC
BstZ17I 5517 GTATAC GTA^TAC
CelII 112 GCTCAGC GC^T[ACGT]AGC
Eam1105I 3993 GACCATTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1649 AGCGCT AGC^GCT
EcoO65I 4740 GGTGACC G^GT[ACGT]ACC
EcoRV 4186 GATATC GAT^ATC
FbaI 2321 TGATCA T^GATCA
FspI 2825 TGCGCA TGC^GCA
HpaI 6339 GTTAAC GTT^AAC
KspI 975 CCGCGG CCGC^GG
MfeI 4553 CAATTG C^AATTG
MroI 4208 TCCGGA T^CCGGA
MunI 4553 CAATTG C^AATTG
NaeI 2947 GCCGGC GCC^GGC
NgoMIV 2947 GCCGGC G^CCGGC
PshBI 3199 ATTAAT AT^TAAT
RcaI 2913 TCATGA T^CATGA
SacI 1519 GAGCTC GAGCT^C
SacII 975 CCGCGG CCGC^GG
ScaI 5087 AGTACT AGT^ACT
SmaI 3179 CCCGGG CCC^GGG
SnaBI 1720 TACGTA TAC^GTA
SspBI 684 TGTACA T^GTACA
SspI 4048 AATATT AAT^ATT
XbaI 6718 TCTAGA T^CTAGA
XmaCI 3179 CCCGGG C^CCGGG
XmaI 3179 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 976 CGCG CG^CG
3767 CGCG
AcyI 2285 GACGCC G[GA]^CG[CT]C
4802 GGCGTC
AflII 37 CTTAAG C^TTAAG
2626 CTTAAG
AocI 4311 CCTGAGG CC^T[ACGT]AGG
7159 CCTGAGG
AspI 672 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
6046 GACCTCGTC
BamHI 4205 GGATCC G^GATCC
6494 GGATCC
BciVI 1631 GGATAC GTATCC(6/5)
5686 GTATCC
BfrI 37 CTTAAG C^TTAAG
2626 CTTAAG
BsaHI 2285 GACGCC G[GA]^CG[CT]C
4802 GGCGTC
BsrBI 5883 CCGCTC CCGCTC(-3/-3)
3835 GAGCGG
BstUI 976 CGCG CG^CG
3767 CGCG
BstXI 824 CCAGTGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4414 CCAACAACTTGG
Bsu36I 4311 CCTGAGG CC^T[ACGT]AGG
7159 CCTGAGG
DraIII 2464 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
5635 CACGAGGTG
Eco81I 4311 CCTGAGG CC^T[ACGT]AGG
7159 CCTGAGG
HaeII 1649 AGCGCT [GA]GCGC^[CT]
5234 GGCGCT
Hin1I 2285 GACGCC G[GA]^CG[CT]C
4802 GGCGTC
HincII 4606 GTTGAC GT[CT]^[GA]AC
6339 GTTAAC
HindII 4606 GTTGAC GT[CT]^[GA]AC
6339 GTTAAC
MvnI 976 CGCG CG^CG
3767 CGCG
NdeI 3485 CATATG CA^TATG
4380 CATATG
NspI 462 GCATGT [GA]CATG^[CT]
2792 ACATGC
PaeR7I 995 CTCGAG C^TCGAG
1241 CTCGAG
PflFI 672 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
6046 GACCTCGTC
SbfI 2925 CCTGCAGG CCTGCA^GG
3261 CCTGCAGG
Sse8387I 2925 CCTGCAGG CCTGCA^GG
3261 CCTGCAGG
Tth111I 672 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
6046 GACCTCGTC
XcmI 3365 CCAGCCTCAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4414 CCAACAACTTGGTGG
XhoI 995 CTCGAG C^TCGAG
1241 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApaI ApoI Asp700I
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvI BcnI BfaI
BglI BglII BmyI BpmI BpuAI
BsaBI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrDI
BsrFI BsrI BssKI BssSI BstAPI
BstF5I BstNI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DrdI DsaI EaeI EarI
EcoNI EcoO109I EcoRI EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
PflMI PleI PpuMI PstI PvuII
RsaI SapI Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII
XmnI