##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII AcyI AgeI AocI Aor51HI
ApaI AscI BbeI BbrPI BbvCI
BcgI BglI BlpI Bpu1102I BsaBI
BsaHI BsiEI BsiWI BspDI BsrBI
BssHII BstEII BstPI Bsu36I CelII
ClaI DraIII EagI EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI HaeII
Hin1I HpaI KasI MamI MfeI
MluI MunI NarI NheI NotI
NruI PacI PaeR7I PinAI PmaCI
PmeI PmlI PvuI SalI SapI
SbfI ScaI SfiI SfoI SgrAI
SnaBI SpeI SphI Sse8387I SwaI
XhoI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AccI 606 GTATAC GT^[AC][GT]AC
AccIII 4188 TCCGGA T^CCGGA
AclI 1151 AACGTT AA^CGTT
AflIII 5248 ACATGT A^C[GA][CT]GT
AhdI 2028 GACTTCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 4515 ATTAAT AT^TAAT
AsnI 4515 ATTAAT AT^TAAT
Asp700I 4004 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2028 GACTTCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 4033 CCTAGG C^CTAGG
BciVI 551 GTATCC GTATCC(6/5)
BclI 3120 TGATCA T^GATCA
BlnI 4033 CCTAGG C^CTAGG
BsaAI 5384 TACGTG [CT]AC^GT[GA]
BsaWI 4188 TCCGGA [AT]^CCGG[AT]
BseAI 4188 TCCGGA T^CCGGA
BsmI 3015 GCATTC GAATGC(1/-1)
Bsp1407I 5251 TGTACA T^GTACA
BspEI 4188 TCCGGA T^CCGGA
BspLU11I 5248 ACATGT A^CATGT
BsrFI 173 GCCGGC [GA]^CCGG[CT]
BsrGI 5251 TGTACA T^GTACA
BssSI 2796 CACGAG CACGAG(-5/-1)
Bst1107I 606 GTATAC GTA^TAC
BstAPI 3489 GCAACCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 521 TTCGAA TT^CGAA
BstZ17I 606 GTATAC GTA^TAC
Cfr10I 173 GCCGGC [GA]^CCGG[CT]
CpoI 2395 CGGTCCG CG^G[AT]CCG
DrdI 4178 GACCATCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2028 GACTTCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 1855 GATATC GAT^ATC
FbaI 3120 TGATCA T^GATCA
FseI 172 GGCCGGCC GGCCGG^CC
HindIII 3683 AAGCTT A^AGCTT
KspI 97 CCGCGG CCGC^GG
MroI 4188 TCCGGA T^CCGGA
NaeI 173 GCCGGC GCC^GGC
NcoI 2770 CCATGG C^CATGG
NgoMIV 173 GCCGGC G^CCGGC
NspV 521 TTCGAA TT^CGAA
PpuMI 3389 GGGTCCC [GA]G^G[AT]CC[CT]
PshBI 4515 ATTAAT AT^TAAT
Psp1406I 1151 AACGTT AA^CGTT
RsrII 2395 CGGTCCG CG^G[AT]CCG
SacI 938 GAGCTC GAGCT^C
SacII 97 CCGCGG CCGC^GG
SexAI 704 ACCAGGT A^CC[AT]GGT
SfuI 521 TTCGAA TT^CGAA
SmaI 476 CCCGGG CCC^GGG
SspBI 5251 TGTACA T^GTACA
SspI 5447 AATATT AAT^ATT
XmaCI 476 CCCGGG C^CCGGG
XmaI 476 CCCGGG C^CCGGG
XmnI 4004 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
Acc65I 619 GGTACC G^GTACC
1639 GGTACC
AflII 4321 CTTAAG C^TTAAG
4864 CTTAAG
Alw44I 292 GTGCAC G^TGCAC
5257 GTGCAC
ApaLI 292 GTGCAC G^TGCAC
5257 GTGCAC
Asp718I 619 GGTACC G^GTACC
1639 GGTACC
AspI 1521 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4785 GACTGAGTC
AvaI 476 CCCGGG C^[CT]CG[GA]G
5192 CTCGGG
AviII 284 TGCGCA TGC^GCA
1844 TGCGCA
BalI 2788 TGGCCA TGG^CCA
3987 TGGCCA
BfrI 4321 CTTAAG C^TTAAG
4864 CTTAAG
BsaI 1127 GAGACC GGTCTC(1/5)
1810 GGTCTC
BsoBI 476 CCCGGG C^[CT]CG[GA]G
5192 CTCGGG
BspHI 4618 TCATGA T^CATGA
5292 TCATGA
BstXI 623 CCAATATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3720 CCAGACTCCTGG
DraII 3389 GGGTCCC [GA]G^G[ACGT]CC[CT]
3700 AGGCCCC
EcoO109I 3389 GGGTCCC [GA]G^G[ACGT]CC[CT]
3700 AGGCCCC
EcoT22I 4877 ATGCAT ATGCA^T
5548 ATGCAT
FauI 96 CCCGC CCCGC(4/6)
1162 GCGGG
FspI 284 TGCGCA TGC^GCA
1844 TGCGCA
HincII 2104 GTCAAC GT[CT]^[GA]AC
3519 GTTGAC
HindII 2104 GTCAAC GT[CT]^[GA]AC
3519 GTTGAC
KpnI 619 GGTACC GGTAC^C
1639 GGTACC
MluNI 2788 TGGCCA TGG^CCA
3987 TGGCCA
MscI 2788 TGGCCA TGG^CCA
3987 TGGCCA
NdeI 2637 CATATG CA^TATG
2993 CATATG
NsiI 4877 ATGCAT ATGCA^T
5548 ATGCAT
NspI 5248 ACATGT [GA]CATG^[CT]
5550 GCATGT
PflFI 1521 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4785 GACTGAGTC
PshAI 1819 GACCCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3443 GACAGCAGTC
RcaI 4618 TCATGA T^CATGA
5292 TCATGA
StuI 3856 AGGCCT AGG^CCT
3944 AGGCCT
Tth111I 1521 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4785 GACTGAGTC
XbaI 3747 TCTAGA T^CTAGA
5142 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaII
BamHI BanI BanII BbsI BbvI
BcnI BfaI BglII BmyI BpmI
BpuAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
Bsp1286I BspMI BsrDI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DsaI EaeI EarI EcoNI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PflMI PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII