##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII Acc65I AccIII AcyI AflII
AgeI AhdI AocI ApaI AscI
Asp718I AspEI AviII AvrII BbeI
BbrPI BcgI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BsaHI BseAI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspEI BsrBI BsrDI BsrGI BssHII
BstAPI Bsu36I CelII ClaI CpoI
DraIII EagI Eam1105I EclXI Eco52I
Eco81I FseI FspI Hin1I KasI
KpnI KspI MluI MroI NarI
NotI NruI PacI PinAI PmaCI
PmeI PmlI PshAI PvuI RsrII
SacI SacII SalI SbfI SexAI
SfiI SfoI SgrAI SnaBI Sse8387I
SspBI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AccII 5174 CGCG CG^CG
AclI 3459 AACGTT AA^CGTT
Alw44I 5407 GTGCAC G^TGCAC
ApaLI 5407 GTGCAC G^TGCAC
AspI 6183 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 3349 GGATCC G^GATCC
BanII 4436 GAGCCC G[GA]GC[CT]^C
BbvCI 6410 CCTCAGC CCTCAGC(-5/-2)
BciVI 6703 GGATAC GTATCC(6/5)
BsaBI 3551 GATACTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspMI 976 GCAGGT ACCTGC(4/8)
BssSI 4686 CTCGTG CACGAG(-5/-1)
Bst1107I 7728 GTATAC GTA^TAC
BstEII 3901 GGTTACC G^GT[ACGT]ACC
BstPI 3901 GGTTACC G^GT[ACGT]ACC
BstUI 5174 CGCG CG^CG
BstZ17I 7728 GTATAC GTA^TAC
EcoO65I 3901 GGTTACC G^GT[ACGT]ACC
HgaI 334 GCGTC GACGC(5/10)
MamI 3551 GATACTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 7789 CAATTG C^AATTG
MunI 7789 CAATTG C^AATTG
MvnI 5174 CGCG CG^CG
NaeI 4606 GCCGGC GCC^GGC
NgoMIV 4606 GCCGGC G^CCGGC
NheI 5305 GCTAGC G^CTAGC
PaeR7I 6285 CTCGAG C^TCGAG
PflFI 6183 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 3459 AACGTT AA^CGTT
SmaI 6253 CCCGGG CCC^GGG
SpeI 4823 ACTAGT A^CTAGT
Tth111I 6183 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 6285 CTCGAG C^TCGAG
XmaCI 6253 CCCGGG C^CCGGG
XmaI 6253 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccI 96 GTAGAC GT^[AC][GT]AC
7728 GTATAC
AvaI 6253 CCCGGG C^[CT]CG[GA]G
6285 CTCGAG
BalI 2038 TGGCCA TGG^CCA
6368 TGGCCA
BglII 3481 AGATCT A^GATCT
5979 AGATCT
BsaWI 3090 ACCGGA [AT]^CCGG[AT]
3502 ACCGGA
BsoBI 6253 CCCGGG C^[CT]CG[GA]G
6285 CTCGAG
BsrFI 1911 ACCGGC [GA]^CCGG[CT]
4606 GCCGGC
BstXI 6420 CCAAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
6791 CCAAGATCCTGG
Cfr10I 1911 ACCGGC [GA]^CCGG[CT]
4606 GCCGGC
DraII 3199 AGGACCT [GA]G^G[ACGT]CC[CT]
5227 AGGTCCT
DsaI 265 CCATGG C^C[GA][CT]GG
5212 CCATGG
EcoO109I 3199 AGGACCT [GA]G^G[ACGT]CC[CT]
5227 AGGTCCT
EcoRI 1235 GAATTC G^AATTC
1256 GAATTC
MluNI 2038 TGGCCA TGG^CCA
6368 TGGCCA
MscI 2038 TGGCCA TGG^CCA
6368 TGGCCA
NcoI 265 CCATGG C^CATGG
5212 CCATGG
NdeI 1709 CATATG CA^TATG
5250 CATATG
PpuMI 3199 AGGACCT [GA]G^G[AT]CC[CT]
5227 AGGTCCT
PstI 3314 CTGCAG CTGCA^G
3399 CTGCAG
SphI 2326 GCATGC GCATG^C
4681 GCATGC
StuI 4049 AGGCCT AGG^CCT
4345 AGGCCT
XcmI 6593 CCATTTTGCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
7486 CCATTTTCACATTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI Aor51HI ApoI AseI
AsnI Asp700I AspHI AvaII BanI
BbsI BbvI BclI BcnI BfaI
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspHI
BspLU11I BsrI BssKI BstBI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DrdI EaeI
EarI Eco47III EcoNI EcoRII EcoRV
EcoT14I EcoT22I FauI FbaI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HincII HindII HindIII HinfI
HpaI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NsiI NspI NspV
PflMI PleI PshBI PvuII RcaI
RsaI SapI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SfuI SmlI
SspI StyI SwaI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XbaI XhoII XmnI