##################################
# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AgeI AhdI Alw44I AocI ApaI
ApaLI AscI Asp718I AspEI AspI
AviII AvrII BamHI BbeI BbrPI
BbvCI BglI BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BspDI
BspEI BspHI BsrBI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraII DraIII
DsaI EagI Eam1105I EclXI Eco52I
Eco81I EcoO109I EcoO65I FauI FseI
FspI HgaI Hin1I KasI KpnI
KspI MamI MfeI MluI MroI
MunI MvnI NaeI NarI NcoI
NgoMIV NheI NotI NruI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PpuMI PvuI PvuII RcaI
RsrII SacII SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SphI Sse8387I StuI SwaI
Tth111I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AclI 109 AACGTT AA^CGTT
Aor51HI 500 AGCGCT AGC^GCT
AspHI 926 GAGCTC G[AT]GC[AT]^C
AvaI 3591 CTCGAG C^[CT]CG[GA]G
AvaII 1355 GGACC G^G[AT]CC
BanI 2995 GGTGCC G^G[CT][GA]CC
BcgI 1837 GCACGTCCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 422 GTATCC GTATCC(6/5)
BclI 122 TGATCA T^GATCA
BcnI 1487 CCCGG CC^[GC]GG
BsiHKAI 926 GAGCTC G[AT]GC[AT]^C
BsmI 3850 GCATTC GAATGC(1/-1)
BsoBI 3591 CTCGAG C^[CT]CG[GA]G
Bsp1407I 1763 TGTACA T^GTACA
BsrGI 1763 TGTACA T^GTACA
BstNI 3062 CCAGG CC^[AT]GG
CfoI 501 GCGC GCG^C
Cfr13I 1355 GGACC G^G[ACGT]CC
DrdI 4908 GACTATGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 500 AGCGCT AGC^GCT
EcoNI 4203 CCTGAAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRII 3062 CCAGG ^CC[AT]GG
EcoRV 1421 GATATC GAT^ATC
FbaI 122 TGATCA T^GATCA
HaeII 500 AGCGCT [GA]GCGC^[CT]
HapII 1488 CCGG C^CGG
HhaI 501 GCGC GCG^C
HinP1I 501 GCGC G^CGC
HincII 4832 GTTAAC GT[CT]^[GA]AC
HindII 4832 GTTAAC GT[CT]^[GA]AC
HindIII 1257 AAGCTT A^AGCTT
HpaI 4832 GTTAAC GTT^AAC
HpaII 1488 CCGG C^CGG
MspA1I 4128 CCGCTG C[AC]G^C[GT]G
MspI 1488 CCGG C^CGG
MvaI 3062 CCAGG CC^[AT]GG
NciI 1487 CCCGG CC^[GC]GG
PaeR7I 3591 CTCGAG C^TCGAG
PflMI 2719 CCATTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 3014 GACTTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 109 AACGTT AA^CGTT
PstI 3501 CTGCAG CTGCA^G
SacI 926 GAGCTC GAGCT^C
SapI 1641 GCTCTTC GCTCTTC(1/4)
Sau96I 1355 GGACC G^G[ACGT]CC
SpeI 4232 ACTAGT A^CTAGT
SspBI 1763 TGTACA T^GTACA
Van91I 2719 CCATTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1537 CCAATTTTAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 3591 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AciI 366 CCGC CCGC(-3/-1)
4128 CCGC
AflII 3585 CTTAAG C^TTAAG
3996 CTTAAG
AlwNI 3453 CAGTATCTG CAG[ACGT][ACGT][ACGT]^CTG
3495 CAGCATCTG
AseI 2354 ATTAAT AT^TAAT
4639 ATTAAT
AsnI 2354 ATTAAT AT^TAAT
4639 ATTAAT
BalI 1091 TGGCCA TGG^CCA
3727 TGGCCA
BanII 926 GAGCTC G[GA]GC[CT]^C
1745 GAGCCC
BfrI 3585 CTTAAG C^TTAAG
3996 CTTAAG
BseRI 478 GAGGAG GAGGAG(10/8)
1335 GAGGAG
BspMI 3412 GCAGGT ACCTGC(4/8)
3662 GCAGGT
BssKI 1487 CCCGG ^CC[ACGT]GG
3062 CCAGG
BstXI 461 CCAAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3158 CCAAAAAACTGG
Cac8I 722 GCCAGC GC[ACGT]^[ACGT]GC
1225 GCAAGC
EaeI 1091 TGGCCA [CT]^GGCC[GA]
3727 TGGCCA
MluNI 1091 TGGCCA TGG^CCA
3727 TGGCCA
MscI 1091 TGGCCA TGG^CCA
3727 TGGCCA
NdeI 204 CATATG CA^TATG
3868 CATATG
NlaIV 1550 GGAGCC GG[ACGT]^[ACGT]CC
2995 GGTGCC
PshBI 2354 ATTAAT AT^TAAT
4639 ATTAAT
ScaI 578 AGTACT AGT^ACT
4992 AGTACT
ScrFI 1487 CCCGG CC^[ACGT]GG
3062 CCAGG
TaqI 1707 TCGA T^CGA
3592 TCGA
TthHB8I 1707 TCGA T^CGA
3592 TCGA
XbaI 1583 TCTAGA T^CTAGA
5083 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AccI AcsI AfaI AflIII AluI
AlwI ApoI Asp700I BbsI BbvI
BfaI BglII BmyI BpmI BpuAI
BsaI BsaJI BsiYI BslI BsmAI
BsmFI Bsp1286I BspLU11I BsrDI BsrI
BstF5I BstYI DdeI DpnII DraI
EarI EcoRI EcoT14I EcoT22I Fnu4HI
FokI HaeIII HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MwoI NdeII NlaIII NsiI NspI
PleI RsaI Sau3AI SfaNI SfcI
SmlI SspI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII
XmnI