##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI Aor51HI ApaI AscI Asp718I
AviII AvrII BamHI BbeI BbrPI
BbvCI BfrI BglII BlnI BsaBI
BsaWI BseAI BsiWI BspDI BspEI
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I ClaI CpoI DraIII DrdI
EagI EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRV FseI FspI HpaI
KasI KpnI MamI MfeI MluI
MroI MunI NarI NdeI NheI
NotI NruI NspV PacI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RsrII SalI SbfI SexAI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I StuI SwaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AcyI 201 GACGCC G[GA]^CG[CT]C
AflIII 2323 ACATGT A^C[GA][CT]GT
AhdI 2008 GACAATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 645 GTGCAC G^TGCAC
ApaLI 645 GTGCAC G^TGCAC
AseI 1638 ATTAAT AT^TAAT
AsnI 1638 ATTAAT AT^TAAT
AspEI 2008 GACAATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 459 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 389 GGTGCC G^G[CT][GA]CC
BcgI 557 CGAGGCTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1492 TGATCA T^GATCA
BsaAI 792 TACGTG [CT]AC^GT[GA]
BsaHI 201 GACGCC G[GA]^CG[CT]C
BsgI 506 CTGCAC GTGCAG(16/14)
BsiEI 1193 CGACCG CG[GA][CT]^CG
BsmBI 120 GAGACG CGTCTC(1/5)
Bsp1407I 2378 TGTACA T^GTACA
BspHI 1838 TCATGA T^CATGA
BspLU11I 2323 ACATGT A^CATGT
BsrBI 1326 GAGCGG CCGCTC(-3/-3)
BsrFI 158 GCCGGC [GA]^CCGG[CT]
BsrGI 2378 TGTACA T^GTACA
BssHII 215 GCGCGC G^CGCGC
BstAPI 59 GCAGCCCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 158 GCCGGC [GA]^CCGG[CT]
DraI 1043 TTTAAA TTT^AAA
Eam1105I 2008 GACAATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 2768 ATGCAT ATGCA^T
FbaI 1492 TGATCA T^GATCA
Hin1I 201 GACGCC G[GA]^CG[CT]C
HincII 2016 GTCAAC GT[CT]^[GA]AC
HindII 2016 GTCAAC GT[CT]^[GA]AC
KspI 9 CCGCGG CCGC^GG
NaeI 158 GCCGGC GCC^GGC
NgoMIV 158 GCCGGC G^CCGGC
NsiI 2768 ATGCAT ATGCA^T
PaeR7I 80 CTCGAG C^TCGAG
PflFI 459 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2958 CCAAGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 1638 ATTAAT AT^TAAT
RcaI 1838 TCATGA T^CATGA
SacII 9 CCGCGG CCGC^GG
ScaI 1871 AGTACT AGT^ACT
SfiI 1065 GGCCGGGCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 3247 CCCGGG CCC^GGG
SspBI 2378 TGTACA T^GTACA
Tth111I 459 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2958 CCAAGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1620 TCTAGA T^CTAGA
XhoI 80 CTCGAG C^TCGAG
XmaCI 3247 CCCGGG C^CCGGG
XmaI 3247 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccI 525 GTCTAC GT^[AC][GT]AC
2005 GTAGAC
AlwI 305 GATCC GGATC(4/5)
533 GATCC
Asp700I 2142 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2887 GAAGAGTTTC
BbsI 2926 GAAGAC GAAGAC(2/6)
880 GTCTTC
BciVI 2341 GGATAC GTATCC(6/5)
2971 GTATCC
BglI 1066 GCCGGGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1799 GCCAATTTGGC
BlpI 681 GCTCAGC GC^T[ACGT]AGC
1474 GCTGAGC
Bpu1102I 681 GCTCAGC GC^T[ACGT]AGC
1474 GCTGAGC
BpuAI 2926 GAAGAC GAAGAC(2/6)
880 GTCTTC
BsrDI 2157 CATTGC GCAATG(2/0)
2700 CATTGC
BssSI 1302 CACGAG CACGAG(-5/-1)
642 CTCGTG
BstYI 304 AGATCC [GA]^GATC[CT]
532 AGATCC
CelII 681 GCTCAGC GC^T[ACGT]AGC
1474 GCTGAGC
EcoRI 1736 GAATTC G^AATTC
3276 GAATTC
HaeII 35 AGCGCC [GA]GCGC^[CT]
84 AGCGCC
HindIII 2847 AAGCTT A^AGCTT
3630 AAGCTT
MflI 304 AGATCC [GA]^GATC[CT]
532 AGATCC
NcoI 88 CCATGG C^CATGG
2237 CCATGG
NspI 2313 GCATGT [GA]CATG^[CT]
2323 ACATGT
PstI 1465 CTGCAG CTGCA^G
2421 CTGCAG
XhoII 304 AGATCC [GA]^GATC[CT]
532 AGATCC
XmnI 2142 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2887 GAAGAGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwNI AocI ApoI AspHI AvaI
AvaII BalI BanII BbvI BcnI
BfaI BmyI BpmI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstUI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI PvuII RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI