##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII AccIII AclI AcyI AflII
AhdI AlwNI Aor51HI ApaI AscI
AspEI BanII BbeI BbrPI BciVI
BfrI BsaAI BsaHI BseAI BsiWI
BsmI BspDI BspEI BsrBI BssHII
BssSI BstBI BstEII BstPI ClaI
CpoI DraIII EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRI FseI
Hin1I HpaI KasI MluI MroI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuII RsrII SacI SalI
SapI ScaI SfoI SfuI SgrAI
SmaI SnaBI StuI SwaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AgeI 2336 ACCGGT A^CCGGT
Alw44I 3468 GTGCAC G^TGCAC
AocI 75 CCTCAGG CC^T[ACGT]AGG
ApaLI 3468 GTGCAC G^TGCAC
AseI 1791 ATTAAT AT^TAAT
AsnI 1791 ATTAAT AT^TAAT
AspI 1301 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1909 TGCGCA TGC^GCA
AvrII 3354 CCTAGG C^CTAGG
BamHI 1556 GGATCC G^GATCC
BbsI 816 GTCTTC GAAGAC(2/6)
BbvCI 2099 CCTCAGC CCTCAGC(-5/-2)
BcgI 2783 CGATGCTTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 4043 TGATCA T^GATCA
BcnI 1525 CCCGG CC^[GC]GG
BlnI 3354 CCTAGG C^CTAGG
BlpI 1817 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 1817 GCTTAGC GC^T[ACGT]AGC
BpuAI 816 GTCTTC GAAGAC(2/6)
BsaBI 30 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2336 ACCGGT [AT]^CCGG[AT]
BsiEI 2020 CGATCG CG[GA][CT]^CG
BsmBI 1617 GAGACG CGTCTC(1/5)
Bsp1407I 712 TGTACA T^GTACA
BspHI 3959 TCATGA T^CATGA
BspLU11I 278 ACATGT A^CATGT
BsrFI 2336 ACCGGT [GA]^CCGG[CT]
BsrGI 712 TGTACA T^GTACA
Bsu36I 75 CCTCAGG CC^T[ACGT]AGG
CelII 1817 GCTTAGC GC^T[ACGT]AGC
Cfr10I 2336 ACCGGT [GA]^CCGG[CT]
DrdI 2373 GACTAAATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 75 CCTCAGG CC^T[ACGT]AGG
EcoNI 3349 CCTAGCCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 3994 GATATC GAT^ATC
EcoT22I 1210 ATGCAT ATGCA^T
FbaI 4043 TGATCA T^GATCA
FspI 1909 TGCGCA TGC^GCA
HaeII 1521 AGCGCC [GA]GCGC^[CT]
MamI 30 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3411 CAATTG C^AATTG
MunI 3411 CAATTG C^AATTG
NciI 1525 CCCGG CC^[GC]GG
NcoI 21 CCATGG C^CATGG
NsiI 1210 ATGCAT ATGCA^T
PflFI 1301 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2336 ACCGGT A^CCGGT
PshBI 1791 ATTAAT AT^TAAT
PvuI 2020 CGATCG CGAT^CG
RcaI 3959 TCATGA T^CATGA
SbfI 1283 CCTGCAGG CCTGCA^GG
SexAI 1401 ACCTGGT A^CC[AT]GGT
SfiI 1733 GGCCACCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 971 ACTAGT A^CTAGT
SphI 147 GCATGC GCATG^C
Sse8387I 1283 CCTGCAGG CCTGCA^GG
SspBI 712 TGTACA T^GTACA
Tth111I 1301 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3600 TCTAGA T^CTAGA
XcmI 1927 CCACTCCTGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
Acc65I 325 GGTACC G^GTACC
1708 GGTACC
AccII 255 CGCG CG^CG
1704 CGCG
AcsI 621 AAATTC [GA]^AATT[CT]
796 AAATTT
AflIII 278 ACATGT A^C[GA][CT]GT
1353 ACGTGT
ApoI 621 AAATTC [GA]^AATT[CT]
796 AAATTT
Asp718I 325 GGTACC G^GTACC
1708 GGTACC
AspHI 1698 GAGCAC G[AT]GC[AT]^C
3468 GTGCAC
AvaI 133 CCCGAG C^[CT]CG[GA]G
2110 CCCGAG
AvaII 485 GGACC G^G[AT]CC
834 GGACC
BanI 325 GGTACC G^G[CT][GA]CC
1708 GGTACC
BsaI 1000 GAGACC GGTCTC(1/5)
2115 GAGACC
BsgI 613 CTGCAC GTGCAG(16/14)
3525 GTGCAG
BsiHKAI 1698 GAGCAC G[AT]GC[AT]^C
3468 GTGCAC
BsoBI 133 CCCGAG C^[CT]CG[GA]G
2110 CCCGAG
BspMI 611 ACCTGC ACCTGC(4/8)
1286 GCAGGT
BsrDI 1022 CATTGC GCAATG(2/0)
960 GCAATG
BstAPI 1286 GCAGGTTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2778 GCACACGATGC
BstUI 255 CGCG CG^CG
1704 CGCG
BstXI 1823 CCACTCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2767 CCAGAAGCATGG
DraII 1719 AGGGCCT [GA]G^G[ACGT]CC[CT]
2025 GGGGCCT
EcoO109I 1719 AGGGCCT [GA]G^G[ACGT]CC[CT]
2025 GGGGCCT
HgaI 306 GACGC GACGC(5/10)
1641 GACGC
HincII 1307 GTCAAC GT[CT]^[GA]AC
2814 GTCAAC
HindII 1307 GTCAAC GT[CT]^[GA]AC
2814 GTCAAC
HindIII 759 AAGCTT A^AGCTT
1197 AAGCTT
KpnI 325 GGTACC GGTAC^C
1708 GGTACC
KspI 254 CCGCGG CCGC^GG
1703 CCGCGG
MspA1I 254 CCGCGG C[AC]G^C[GT]G
1703 CCGCGG
MvnI 255 CGCG CG^CG
1704 CGCG
PflMI 837 CCATATAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3098 CCACCTTTTGG
PleI 249 GACTC GAGTC(4/5)
1077 GAGTC
SacII 254 CCGCGG CCGC^GG
1703 CCGCGG
SmlI 1997 CTTGAG C^T[CT][GA]AG
2290 CTTGAG
SspI 2905 AATATT AAT^ATT
2940 AATATT
Tsp45I 196 GTGAC ^GT[GC]AC
1979 GTCAC
Van91I 837 CCATATAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3098 CCACCTTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccI AciI AfaI AluI AlwI
Asp700I BalI BbvI BfaI BglI
BglII BmyI BpmI BsaJI BseRI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI Bst1107I BstF5I BstNI
BstYI BstZ17I Cac8I CfoI Cfr13I
DdeI DpnII DraI DsaI EaeI
EarI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspI MvaI MwoI NdeII
NlaIII NlaIV NspI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I XhoII XmnI