##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII AccIII AclI AhdI Alw44I
Aor51HI ApaI ApaLI AscI AspEI
AspI AviII AvrII BamHI BbeI
BbrPI BcgI BglII BlnI BsaAI
BseAI BsiEI BsiWI BspEI BspLU11I
BsrBI BssHII BssSI BstBI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoRI EcoT22I
FseI FspI HpaI KasI KspI
MroI NaeI NarI NdeI NgoMIV
NotI NruI NsiI NspV PacI
PflFI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RsrII SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SspI
SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
Acc65I 1317 GGTACC G^GTACC
AccII 2249 CGCG CG^CG
AcyI 1289 GACGCC G[GA]^CG[CT]C
AflII 1734 CTTAAG C^TTAAG
AflIII 2248 ACGCGT A^C[GA][CT]GT
AgeI 2237 ACCGGT A^CCGGT
AseI 2290 ATTAAT AT^TAAT
AsnI 2290 ATTAAT AT^TAAT
Asp718I 1317 GGTACC G^GTACC
BbvCI 3432 CCTCAGC CCTCAGC(-5/-2)
BfrI 1734 CTTAAG C^TTAAG
BlpI 1793 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1793 GCTCAGC GC^T[ACGT]AGC
BsaHI 1289 GACGCC G[GA]^CG[CT]C
BsaI 155 GGTCTC GGTCTC(1/5)
BsmBI 1603 CGTCTC CGTCTC(1/5)
Bsp1407I 2028 TGTACA T^GTACA
BspDI 78 ATCGAT AT^CGAT
BsrFI 2237 ACCGGT [GA]^CCGG[CT]
BsrGI 2028 TGTACA T^GTACA
BstEII 1722 GGTCACC G^GT[ACGT]ACC
BstPI 1722 GGTCACC G^GT[ACGT]ACC
BstUI 2249 CGCG CG^CG
CelII 1793 GCTCAGC GC^T[ACGT]AGC
CfoI 1116 GCGC GCG^C
Cfr10I 2237 ACCGGT [GA]^CCGG[CT]
ClaI 78 ATCGAT AT^CGAT
EcoO65I 1722 GGTCACC G^GT[ACGT]ACC
HaeII 1115 GGCGCT [GA]GCGC^[CT]
HhaI 1116 GCGC GCG^C
Hin1I 1289 GACGCC G[GA]^CG[CT]C
HinP1I 1116 GCGC G^CGC
HincII 214 GTCAAC GT[CT]^[GA]AC
HindII 214 GTCAAC GT[CT]^[GA]AC
KpnI 1317 GGTACC GGTAC^C
MfeI 398 CAATTG C^AATTG
MluI 2248 ACGCGT A^CGCGT
MunI 398 CAATTG C^AATTG
MvnI 2249 CGCG CG^CG
NheI 2589 GCTAGC G^CTAGC
PflMI 3623 CCACTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 2237 ACCGGT A^CCGGT
PshBI 2290 ATTAAT AT^TAAT
PvuII 2032 CAGCTG CAG^CTG
SapI 760 GCTCTTC GCTCTTC(1/4)
ScaI 2815 AGTACT AGT^ACT
SmaI 1622 CCCGGG CCC^GGG
SspBI 2028 TGTACA T^GTACA
StuI 830 AGGCCT AGG^CCT
Van91I 3623 CCACTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 528 TCTAGA T^CTAGA
XmaCI 1622 CCCGGG C^CCGGG
XmaI 1622 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 637 GTATAC GT^[AC][GT]AC
2224 GTATAC
AlwNI 1973 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
2197 CAGCTCCTG
Asp700I 949 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1976 GAACTGCTTC
BanI 1317 GGTACC G^G[CT][GA]CC
2317 GGTGCC
BciVI 171 GGATAC GTATCC(6/5)
1940 GTATCC
BglI 2707 GCCCAGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3050 GCCTTTTTGGC
BsaBI 1497 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2802 GATGTGTATC
BsaWI 38 ACCGGA [AT]^CCGG[AT]
2237 ACCGGT
BsgI 1261 GTGCAG GTGCAG(16/14)
3166 GTGCAG
BsmI 2115 GAATGC GAATGC(1/-1)
2628 GAATGC
BspHI 659 TCATGA T^CATGA
3180 TCATGA
Bst1107I 637 GTATAC GTA^TAC
2224 GTATAC
BstZ17I 637 GTATAC GTA^TAC
2224 GTATAC
DsaI 296 CCATGG C^C[GA][CT]GG
1775 CCATGG
EcoRV 1396 GATATC GAT^ATC
3214 GATATC
HindIII 3440 AAGCTT A^AGCTT
3652 AAGCTT
MamI 1497 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2802 GATGTGTATC
MspA1I 2032 CAGCTG C[AC]G^C[GT]G
3639 CAGCGG
NcoI 296 CCATGG C^CATGG
1775 CCATGG
RcaI 659 TCATGA T^CATGA
3180 TCATGA
SacI 568 GAGCTC GAGCT^C
1692 GAGCTC
XcmI 2969 CCAGTCCCAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3473 CCATGAATATCCTGG
XmnI 949 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1976 GAACTGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AocI ApoI AspHI AvaI AvaII
BalI BanII BbsI BbvI BclI
BcnI BfaI BmyI BpmI BpuAI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrDI BsrI BssKI BstAPI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
Cfr13I DdeI DpnII DraII EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeIII HapII HgaI HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PaeR7I PleI PpuMI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SphI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoI XhoII