################################## # NO CUT ENZYMES ( 92 enzymes) # ################################## AatII Acc65I AccIII AclI AgeI AhdI Alw44I Aor51HI ApaLI AscI Asp718I AspEI AspI AviII AvrII BamHI BbrPI BcgI BglI BglII BlnI BsaI BsaWI BseAI BsiEI BsiWI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrFI BsrGI BssHII BstBI BstEII BstPI Cfr10I ClaI CpoI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI FseI FspI HgaI HpaI KpnI KspI MluI MroI NaeI NcoI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmlI PpuMI PshAI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SphI Sse8387I SspBI StuI SwaI TaqI Tth111I TthHB8I XhoI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AflII 3357 CTTAAG C^TTAAG ApaI 171 GGGCCC GGGCC^C Asp700I 556 GAACTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BbsI 3537 GAAGAC GAAGAC(2/6) BbvCI 176 CCTCAGC CCTCAGC(-5/-2) BciVI 2694 GTATCC GTATCC(6/5) BclI 3287 TGATCA T^GATCA BfrI 3357 CTTAAG C^TTAAG BlpI 270 GCTGAGC GC^T[ACGT]AGC Bpu1102I 270 GCTGAGC GC^T[ACGT]AGC BpuAI 3537 GAAGAC GAAGAC(2/6) BsaAI 2991 TACGTG [CT]AC^GT[GA] BsmBI 95 GAGACG CGTCTC(1/5) BspHI 333 TCATGA T^CATGA BspMI 302 ACCTGC ACCTGC(4/8) BssSI 190 CACGAG CACGAG(-5/-1) BstXI 1155 CCAGTTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 3635 GGATCT [GA]^GATC[CT] CelII 270 GCTGAGC GC^T[ACGT]AGC DsaI 1577 CCACGG C^C[GA][CT]GG EcoNI 303 CCTGCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 3717 GATATC GAT^ATC FbaI 3287 TGATCA T^GATCA HincII 2044 GTTGAC GT[CT]^[GA]AC HindII 2044 GTTGAC GT[CT]^[GA]AC HindIII 1653 AAGCTT A^AGCTT MfeI 2295 CAATTG C^AATTG MflI 3635 GGATCT [GA]^GATC[CT] MunI 2295 CAATTG C^AATTG PmeI 1115 GTTTAAAC GTTT^AAAC RcaI 333 TCATGA T^CATGA SacI 754 GAGCTC GAGCT^C SapI 814 GAAGAGC GCTCTTC(1/4) SmlI 3357 CTTAAG C^T[CT][GA]AG SpeI 3250 ACTAGT A^CTAGT XcmI 1577 CCACGGTGGGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 3635 GGATCT [GA]^GATC[CT] XmnI 556 GAACTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 39 enzymes) # ################################### AcyI 66 GGCGCC G[GA]^CG[CT]C 196 GGCGCC AflIII 1050 ACGTGT A^C[GA][CT]GT 2992 ACGTGT AlwI 3118 GATCC GGATC(4/5) 3635 GGATC AocI 229 CCTGAGG CC^T[ACGT]AGG 2139 CCTAAGG AseI 1302 ATTAAT AT^TAAT 3615 ATTAAT AsnI 1302 ATTAAT AT^TAAT 3615 ATTAAT AvaI 1 CCCGGG C^[CT]CG[GA]G 70 CCCGGG BalI 615 TGGCCA TGG^CCA 3095 TGGCCA BanI 66 GGCGCC G^G[CT][GA]CC 196 GGCGCC BbeI 66 GGCGCC GGCGC^C 196 GGCGCC BsaBI 733 GATGTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3636 GATCTCAATC BsaHI 66 GGCGCC G[GA]^CG[CT]C 196 GGCGCC BsoBI 1 CCCGGG C^[CT]CG[GA]G 70 CCCGGG BsrDI 1524 CATTGC GCAATG(2/0) 1932 CATTGC Bst1107I 2107 GTATAC GTA^TAC 3859 GTATAC BstAPI 252 GCAGCGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 712 GCACTCTTTGC BstZ17I 2107 GTATAC GTA^TAC 3859 GTATAC Bsu36I 229 CCTGAGG CC^T[ACGT]AGG 2139 CCTAAGG DrdI 472 GACTTAATAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2678 GACAGTGATGTC EaeI 615 TGGCCA [CT]^GGCC[GA] 3095 TGGCCA Eco81I 229 CCTGAGG CC^T[ACGT]AGG 2139 CCTAAGG EcoT14I 2689 CCAAGG C^C[AT][AT]GG 3542 CCTTGG Hin1I 66 GGCGCC G[GA]^CG[CT]C 196 GGCGCC KasI 66 GGCGCC G^GCGCC 196 GGCGCC MamI 733 GATGTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3636 GATCTCAATC MluNI 615 TGGCCA TGG^CCA 3095 TGGCCA MscI 615 TGGCCA TGG^CCA 3095 TGGCCA NarI 66 GGCGCC GG^CGCC 196 GGCGCC PflMI 1945 CCACCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2020 CCAGAAGGTGG PshBI 1302 ATTAAT AT^TAAT 3615 ATTAAT PstI 250 CTGCAG CTGCA^G 2150 CTGCAG PvuII 1361 CAGCTG CAG^CTG 1484 CAGCTG SfoI 66 GGCGCC GGC^GCC 196 GGCGCC SmaI 1 CCCGGG CCC^GGG 70 CCCGGG StyI 2689 CCAAGG C^C[AT][AT]GG 3542 CCTTGG Van91I 1945 CCACCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2020 CCAGAAGGTGG XbaI 3576 TCTAGA T^CTAGA 3740 TCTAGA XmaCI 1 CCCGGG C^CCGGG 70 CCCGGG XmaI 1 CCCGGG C^CCGGG 70 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 89 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AluI AlwNI ApoI AspHI AvaII BanII BbvI BcnI BfaI BmyI BpmI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII EarI EcoO109I EcoRII EcoT22I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeI NdeII NlaIII NlaIV NsiI NspI PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SspI TfiI Tru9I TseI Tsp45I Tsp509I TspRI