##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII Acc65I AclI AcyI AflII
AgeI AhdI AocI Aor51HI AscI
Asp718I AspEI AspI BbeI BbrPI
BbvCI BfrI BglI BsaAI BsaHI
BsgI BsiWI BspDI BssHII BssSI
BstEII BstPI Bsu36I ClaI CpoI
DraIII Eam1105I Eco47III Eco81I EcoO65I
EcoRV EcoT22I Hin1I KasI KpnI
NarI NdeI NheI NotI NruI
NsiI PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI Psp1406I PvuI
RsrII SbfI ScaI SexAI SfiI
SfoI SnaBI SphI Sse8387I SspI
SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AccIII 2175 TCCGGA T^CCGGA
Alw44I 1351 GTGCAC G^TGCAC
AlwNI 3488 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 2024 GGGCCC GGGCC^C
ApaLI 1351 GTGCAC G^TGCAC
AviII 1045 TGCGCA TGC^GCA
AvrII 2011 CCTAGG C^CTAGG
BalI 3566 TGGCCA TGG^CCA
BamHI 3509 GGATCC G^GATCC
BbsI 3534 GAAGAC GAAGAC(2/6)
BcgI 3621 GCAGCACCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2849 GGATAC GTATCC(6/5)
BclI 1906 TGATCA T^GATCA
BglII 1076 AGATCT A^GATCT
BlnI 2011 CCTAGG C^CTAGG
BpuAI 3534 GAAGAC GAAGAC(2/6)
BsaBI 1884 GATTTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 3418 GAGACC GGTCTC(1/5)
BseAI 2175 TCCGGA T^CCGGA
BspEI 2175 TCCGGA T^CCGGA
BspLU11I 3238 ACATGT A^CATGT
BsrDI 1266 GCAATG GCAATG(2/0)
Bst1107I 2398 GTATAC GTA^TAC
BstAPI 109 GCAAGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1140 TTCGAA TT^CGAA
BstZ17I 2398 GTATAC GTA^TAC
DraI 1823 TTTAAA TTT^AAA
DrdI 3049 GACCTCAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1095 CGGCCG C^GGCCG
EclXI 1095 CGGCCG C^GGCCG
Eco52I 1095 CGGCCG C^GGCCG
FbaI 1906 TGATCA T^GATCA
FseI 1092 GGCCGGCC GGCCGG^CC
FspI 1045 TGCGCA TGC^GCA
HindIII 1306 AAGCTT A^AGCTT
HpaI 3796 GTTAAC GTT^AAC
MamI 1884 GATTTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1041 CAATTG C^AATTG
MluI 296 ACGCGT A^CGCGT
MluNI 3566 TGGCCA TGG^CCA
MroI 2175 TCCGGA T^CCGGA
MscI 3566 TGGCCA TGG^CCA
MunI 1041 CAATTG C^AATTG
NaeI 1093 GCCGGC GCC^GGC
NcoI 3704 CCATGG C^CATGG
NgoMIV 1093 GCCGGC G^CCGGC
NspV 1140 TTCGAA TT^CGAA
PflMI 351 CCAACTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 3876 GACTC GAGTC(4/5)
SfuI 1140 TTCGAA TT^CGAA
SmaI 654 CCCGGG CCC^GGG
SpeI 2769 ACTAGT A^CTAGT
StuI 2668 AGGCCT AGG^CCT
Van91I 351 CCAACTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2841 TCTAGA T^CTAGA
XmaCI 654 CCCGGG C^CCGGG
XmaI 654 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AflIII 296 ACGCGT A^C[GA][CT]GT
3238 ACATGT
AseI 1656 ATTAAT AT^TAAT
3670 ATTAAT
AsnI 1656 ATTAAT AT^TAAT
3670 ATTAAT
BlpI 2898 GCTCAGC GC^T[ACGT]AGC
3076 GCTGAGC
BpmI 3401 CTCCAG CTGGAG(16/14)
741 CTGGAG
Bpu1102I 2898 GCTCAGC GC^T[ACGT]AGC
3076 GCTGAGC
BsiEI 719 CGACCG CG[GA][CT]^CG
1095 CGGCCG
BsmI 2745 GAATGC GAATGC(1/-1)
1364 GCATTC
Bsp1407I 3233 TGTACA T^GTACA
3867 TGTACA
BsrBI 2614 CCGCTC CCGCTC(-3/-3)
2845 GAGCGG
BsrGI 3233 TGTACA T^GTACA
3867 TGTACA
BstXI 248 CCAGGGCGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3398 CCACTCCAGTGG
CelII 2898 GCTCAGC GC^T[ACGT]AGC
3076 GCTGAGC
EcoNI 304 CCTCGCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2163 CCTGCGTGAGG
EcoRI 1931 GAATTC G^AATTC
2474 GAATTC
HaeII 258 GGCGCT [GA]GCGC^[CT]
612 GGCGCT
PshAI 28 GACTGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2362 GACAATCGTC
PshBI 1656 ATTAAT AT^TAAT
3670 ATTAAT
SacI 2419 GAGCTC GAGCT^C
2992 GAGCTC
SalI 3409 GTCGAC G^TCGAC
3462 GTCGAC
SapI 427 GAAGAGC GCTCTTC(1/4)
2059 GCTCTTC
SgrAI 120 CGCCGGTG C[GA]^CCGG[CT]G
673 CACCGGCG
SmlI 2259 CTTGAG C^T[CT][GA]AG
2559 CTTGAG
SspBI 3233 TGTACA T^GTACA
3867 TGTACA
XcmI 3403 CCAGTGGTCGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3569 CCATCACACTCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI ApoI Asp700I AspHI
AvaI AvaII BanI BanII BbvI
BcnI BfaI BmyI BsaJI BsaWI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspHI
BspMI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PpuMI PstI PvuII RcaI
RsaI SacII Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI