##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII AccIII AclI AgeI AocI
Aor51HI ApaI AscI AspI AviII
AvrII BamHI BlnI BseAI BsiEI
BsiWI BsmBI BspDI BspEI BsrFI
BssHII Bst1107I BstAPI BstZ17I Bsu36I
Cfr10I ClaI CpoI EagI EclXI
Eco47III Eco52I Eco81I EcoNI EcoRV
EcoT22I FseI FspI HpaI MfeI
MluI MroI MunI NaeI NdeI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PflFI PflMI PinAI
PmeI PshAI Psp1406I PvuI RsrII
SacI SalI SapI SbfI SfiI
SgrAI SnaBI SpeI SphI Sse8387I
SwaI Tth111I Van91I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
Acc65I 3365 GGTACC G^GTACC
Alw44I 1428 GTGCAC G^TGCAC
AlwNI 2452 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1428 GTGCAC G^TGCAC
AseI 3440 ATTAAT AT^TAAT
AsnI 3440 ATTAAT AT^TAAT
Asp718I 3365 GGTACC G^GTACC
BbeI 2 GGCGCC GGCGC^C
BbrPI 3391 CACGTG CAC^GTG
BbsI 1563 GAAGAC GAAGAC(2/6)
BbvCI 3825 GCTGAGG CCTCAGC(-5/-2)
BcgI 2787 CGAAATCCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 3281 TGATCA T^GATCA
BlpI 872 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 872 GCTGAGC GC^T[ACGT]AGC
BpuAI 1563 GAAGAC GAAGAC(2/6)
BsaAI 3391 CACGTG [CT]AC^GT[GA]
BsaBI 1220 GATAGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1407I 751 TGTACA T^GTACA
BspHI 334 TCATGA T^CATGA
BsrBI 79 CCGCTC CCGCTC(-3/-3)
BsrGI 751 TGTACA T^GTACA
BstEII 1999 GGTCACC G^GT[ACGT]ACC
BstPI 1999 GGTCACC G^GT[ACGT]ACC
CelII 872 GCTGAGC GC^T[ACGT]AGC
DrdI 2023 GACTCTCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1700 CTCTTC CTCTTC(1/4)
EcoO65I 1999 GGTCACC G^GT[ACGT]ACC
EcoRI 2885 GAATTC G^AATTC
FbaI 3281 TGATCA T^GATCA
HindIII 2572 AAGCTT A^AGCTT
KasI 2 GGCGCC G^GCGCC
KpnI 3365 GGTACC GGTAC^C
Ksp632I 1700 CTCTTC CTCTTC(1/4)
KspI 55 CCGCGG CCGC^GG
MamI 1220 GATAGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 2 GGCGCC GG^CGCC
PmaCI 3391 CACGTG CAC^GTG
PmlI 3391 CACGTG CAC^GTG
PshBI 3440 ATTAAT AT^TAAT
RcaI 334 TCATGA T^CATGA
SacII 55 CCGCGG CCGC^GG
ScaI 3455 AGTACT AGT^ACT
SexAI 2640 ACCTGGT A^CC[AT]GGT
SfoI 2 GGCGCC GGC^GCC
SspBI 751 TGTACA T^GTACA
SspI 2917 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 1512 GTCTAC GT^[AC][GT]AC
3259 GTCTAC
AcyI 2 GGCGCC G[GA]^CG[CT]C
1648 GACGCC
AflII 2048 CTTAAG C^TTAAG
3708 CTTAAG
AhdI 1668 GACAGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1928 GACGTTGTGTC
Asp700I 338 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1346 GAAAACCTTC
AspEI 1668 GACAGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1928 GACGTTGTGTC
BciVI 489 GGATAC GTATCC(6/5)
1058 GTATCC
BfrI 2048 CTTAAG C^TTAAG
3708 CTTAAG
BglII 2362 AGATCT A^GATCT
3504 AGATCT
BsaHI 2 GGCGCC G[GA]^CG[CT]C
1648 GACGCC
BsaI 2238 GAGACC GGTCTC(1/5)
3725 GAGACC
BsaWI 202 TCCGGT [AT]^CCGG[AT]
1516 ACCGGA
BspLU11I 3400 ACATGT A^CATGT
3587 ACATGT
BspMI 3048 ACCTGC ACCTGC(4/8)
2829 GCAGGT
BsrDI 272 CATTGC GCAATG(2/0)
588 GCAATG
BssSI 1103 CACGAG CACGAG(-5/-1)
1404 CTCGTG
BstBI 2160 TTCGAA TT^CGAA
2785 TTCGAA
Eam1105I 1668 GACAGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1928 GACGTTGTGTC
HaeII 2 GGCGCC [GA]GCGC^[CT]
176 GGCGCT
HgaI 585 GACGC GACGC(5/10)
1648 GACGC
Hin1I 2 GGCGCC G[GA]^CG[CT]C
1648 GACGCC
NcoI 1486 CCATGG C^CATGG
1652 CCATGG
NspV 2160 TTCGAA TT^CGAA
2785 TTCGAA
PvuII 1912 CAGCTG CAG^CTG
1979 CAGCTG
SfuI 2160 TTCGAA TT^CGAA
2785 TTCGAA
SmaI 949 CCCGGG CCC^GGG
2306 CCCGGG
SmlI 2048 CTTAAG C^T[CT][GA]AG
3708 CTTAAG
StuI 256 AGGCCT AGG^CCT
1759 AGGCCT
XmaCI 949 CCCGGG C^CCGGG
2306 CCCGGG
XmaI 949 CCCGGG C^CCGGG
2306 CCCGGG
XmnI 338 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1346 GAAAACCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI ApoI AspHI AvaI
AvaII BalI BanI BanII BbvI
BcnI BfaI BglI BmyI BpmI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DraIII DsaI EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII