##################################
# NO CUT ENZYMES ( 49 enzymes) #
##################################
AccI AflII AhdI AscI AspEI
AviII BbeI BciVI BfrI BsaBI
BsiWI Bsp1407I BsrBI BsrGI Bst1107I
BstZ17I CpoI Eam1105I EcoRV EcoT22I
FseI FspI HindIII KasI MamI
MfeI MluI MunI NarI NruI
NsiI PacI PaeR7I PmeI PshAI
PvuI RsrII SacI SalI SbfI
SexAI SfiI SfoI SnaBI SpeI
Sse8387I SspBI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AccIII 3682 TCCGGA T^CCGGA
AclI 3635 AACGTT AA^CGTT
Alw44I 3287 GTGCAC G^TGCAC
Aor51HI 3067 AGCGCT AGC^GCT
ApaI 5127 GGGCCC GGGCC^C
ApaLI 3287 GTGCAC G^TGCAC
Asp700I 4917 GAATCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 4479 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1546 CCTAGG C^CTAGG
BbrPI 4253 CACGTG CAC^GTG
BglII 2053 AGATCT A^GATCT
BlnI 1546 CCTAGG C^CTAGG
BsaAI 4253 CACGTG [CT]AC^GT[GA]
BsaI 3547 GGTCTC GGTCTC(1/5)
BseAI 3682 TCCGGA T^CCGGA
BsiEI 5312 CGGCCG CG[GA][CT]^CG
BsmBI 2476 GAGACG CGTCTC(1/5)
BsmI 1197 GCATTC GAATGC(1/-1)
BspDI 2513 ATCGAT AT^CGAT
BspEI 3682 TCCGGA T^CCGGA
BssHII 3715 GCGCGC G^CGCGC
BstBI 4703 TTCGAA TT^CGAA
BstEII 910 GGTAACC G^GT[ACGT]ACC
BstPI 910 GGTAACC G^GT[ACGT]ACC
ClaI 2513 ATCGAT AT^CGAT
DraI 2124 TTTAAA TTT^AAA
DraIII 3281 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4366 GACCCTGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 5312 CGGCCG C^GGCCG
EclXI 5312 CGGCCG C^GGCCG
Eco47III 3067 AGCGCT AGC^GCT
Eco52I 5312 CGGCCG C^GGCCG
EcoO65I 910 GGTAACC G^GT[ACGT]ACC
HpaI 4581 GTTAAC GTT^AAC
KspI 4990 CCGCGG CCGC^GG
MroI 3682 TCCGGA T^CCGGA
NaeI 2972 GCCGGC GCC^GGC
NdeI 3589 CATATG CA^TATG
NgoMIV 2972 GCCGGC G^CCGGC
NheI 2152 GCTAGC G^CTAGC
NotI 5311 GCGGCCGC GC^GGCCGC
NspV 4703 TTCGAA TT^CGAA
PflFI 4479 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 4253 CACGTG CAC^GTG
PmlI 4253 CACGTG CAC^GTG
Psp1406I 3635 AACGTT AA^CGTT
SacII 4990 CCGCGG CCGC^GG
SfuI 4703 TTCGAA TT^CGAA
SgrAI 2702 CGCCGGTG C[GA]^CCGG[CT]G
SphI 3332 GCATGC GCATG^C
SspI 4348 AATATT AAT^ATT
Tth111I 4479 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3352 TCTAGA T^CTAGA
XmnI 4917 GAATCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AatII 2937 GACGTC GACGT^C
4372 GACGTC
Acc65I 2238 GGTACC G^GTACC
2329 GGTACC
AgeI 2241 ACCGGT A^CCGGT
3994 ACCGGT
AocI 3502 CCTCAGG CC^T[ACGT]AGG
4673 CCTCAGG
AseI 436 ATTAAT AT^TAAT
3577 ATTAAT
AsnI 436 ATTAAT AT^TAAT
3577 ATTAAT
Asp718I 2238 GGTACC G^GTACC
2329 GGTACC
BalI 1812 TGGCCA TGG^CCA
5040 TGGCCA
BamHI 997 GGATCC G^GATCC
3685 GGATCC
BanII 3229 GAGCCC G[GA]GC[CT]^C
5127 GGGCCC
BclI 2978 TGATCA T^GATCA
3054 TGATCA
BlpI 4995 GCTCAGC GC^T[ACGT]AGC
5202 GCTCAGC
Bpu1102I 4995 GCTCAGC GC^T[ACGT]AGC
5202 GCTCAGC
BspLU11I 2378 ACATGT A^CATGT
4859 ACATGT
BsrDI 745 GCAATG GCAATG(2/0)
4799 GCAATG
BstAPI 976 GCATTAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2728 GCACCTGTTGC
Bsu36I 3502 CCTCAGG CC^T[ACGT]AGG
4673 CCTCAGG
CelII 4995 GCTCAGC GC^T[ACGT]AGC
5202 GCTCAGC
Eco81I 3502 CCTCAGG CC^T[ACGT]AGG
4673 CCTCAGG
EcoNI 916 CCTGCCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1079 CCTGGGGAAGG
EcoRI 3345 GAATTC G^AATTC
4312 GAATTC
FbaI 2978 TGATCA T^GATCA
3054 TGATCA
KpnI 2238 GGTACC GGTAC^C
2329 GGTACC
MluNI 1812 TGGCCA TGG^CCA
5040 TGGCCA
MscI 1812 TGGCCA TGG^CCA
5040 TGGCCA
MslI 2991 CACAGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3984 CATGGACATG
PinAI 2241 ACCGGT A^CCGGT
3994 ACCGGT
PshBI 436 ATTAAT AT^TAAT
3577 ATTAAT
SapI 2717 GAAGAGC GCTCTTC(1/4)
3226 GAAGAGC
SmaI 1749 CCCGGG CCC^GGG
5260 CCCGGG
XmaCI 1749 CCCGGG C^CCGGG
5260 CCCGGG
XmaI 1749 CCCGGG C^CCGGG
5260 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
AspHI AvaI AvaII BanI BbsI
BbvCI BbvI BcgI BcnI BfaI
BglI BmyI BpmI BpuAI BsaHI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspHI BspMI BsrFI BsrI
BssKI BssSI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RcaI RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII