##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII AccIII AclI AcsI AflII
AflIII Alw44I ApaLI ApoI AseI
AsnI AviII AvrII BciVI BfrI
BlnI BsaBI BseAI BsiWI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrGI
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI DraIII DrdI
EcoO65I EcoRI EcoRV FseI FspI
HindIII HpaI MamI MfeI MluI
MroI MunI NdeI NheI NruI
NspV PacI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SalI
SapI SexAI SfuI SgrAI SnaBI
SpeI SphI SspBI SspI SwaI
XbaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccI 166 GTCTAC GT^[AC][GT]AC
AgeI 2019 ACCGGT A^CCGGT
AhdI 3821 GACGGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 3815 AGCGCT AGC^GCT
AscI 3893 GGCGCGCC GG^CGCGCC
Asp700I 1587 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 3821 GACGGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 160 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 2649 GGATCC G^GATCC
BbrPI 1137 CACGTG CAC^GTG
BbsI 2335 GAAGAC GAAGAC(2/6)
BcgI 3868 GCAGAGGCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1818 TGATCA T^GATCA
BglII 2207 AGATCT A^GATCT
BpuAI 2335 GAAGAC GAAGAC(2/6)
BsaAI 1137 CACGTG [CT]AC^GT[GA]
BsaWI 2019 ACCGGT [AT]^CCGG[AT]
BsgI 217 GTGCAG GTGCAG(16/14)
BsiEI 3975 CGGCCG CG[GA][CT]^CG
BsmI 2455 GCATTC GAATGC(1/-1)
BssHII 3894 GCGCGC G^CGCGC
BstAPI 1953 GCAGTTATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraI 1865 TTTAAA TTT^AAA
EagI 3975 CGGCCG C^GGCCG
Eam1105I 3821 GACGGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 3975 CGGCCG C^GGCCG
Eco47III 3815 AGCGCT AGC^GCT
Eco52I 3975 CGGCCG C^GGCCG
EcoT22I 2453 ATGCAT ATGCA^T
FbaI 1818 TGATCA T^GATCA
HincII 1804 GTCAAC GT[CT]^[GA]AC
HindII 1804 GTCAAC GT[CT]^[GA]AC
KspI 3076 CCGCGG CCGC^GG
NotI 3974 GCGGCCGC GC^GGCCGC
NsiI 2453 ATGCAT ATGCA^T
PflFI 160 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2019 ACCGGT A^CCGGT
PmaCI 1137 CACGTG CAC^GTG
PmlI 1137 CACGTG CAC^GTG
SacII 3076 CCGCGG CCGC^GG
ScaI 3360 AGTACT AGT^ACT
SfiI 642 GGCCGAGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Tth111I 160 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 1587 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
Acc65I 783 GGTACC G^GTACC
3088 GGTACC
AocI 2504 CCTGAGG CC^T[ACGT]AGG
3120 CCTGAGG
Asp718I 783 GGTACC G^GTACC
3088 GGTACC
BsmBI 3600 CGTCTC CGTCTC(1/5)
1602 GAGACG
BstYI 2207 AGATCT [GA]^GATC[CT]
2649 GGATCC
Bsu36I 2504 CCTGAGG CC^T[ACGT]AGG
3120 CCTGAGG
Eco81I 2504 CCTGAGG CC^T[ACGT]AGG
3120 CCTGAGG
KpnI 783 GGTACC GGTAC^C
3088 GGTACC
MflI 2207 AGATCT [GA]^GATC[CT]
2649 GGATCC
NcoI 394 CCATGG C^CATGG
3182 CCATGG
PaeR7I 362 CTCGAG C^TCGAG
3065 CTCGAG
SfaNI 2921 GATGC GCATC(5/9)
3296 GCATC
TfiI 1045 GATTC G^A[AT]TC
3543 GAATC
XcmI 341 CCACTCAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3238 CCACCTCCACCCTGG
XhoI 362 CTCGAG C^TCGAG
3065 CTCGAG
XhoII 2207 AGATCT [GA]^GATC[CT]
2649 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BalI BanI BanII BbeI
BbvCI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BsaHI
BsaI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrDI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstXI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NdeII
NgoMIV NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I SbfI ScrFI
SfcI SfoI SmaI SmlI Sse8387I
StuI StyI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XmaCI XmaI