##################################
# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI AhdI AocI Aor51HI ApaI
AscI Asp718I AspEI AviII BalI
BamHI BanI BanII BbeI BbvCI
BcgI BciVI BglI BglII BlpI
Bpu1102I BsaBI BsaHI BsaWI BseAI
BsiEI BsiWI BspEI BspLU11I BspMI
BsrBI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstPI BstYI Bsu36I
CelII Cfr10I CpoI DraII DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV FauI FseI FspI
HaeII HgaI Hin1I HpaI KasI
KpnI KspI MamI MflI MluI
MluNI MroI MscI MspA1I NaeI
NarI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PinAI PmeI
PpuMI PshAI Psp1406I PvuI PvuII
RsrII SacI SacII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I StuI SwaI
XhoI XhoII
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccI 3707 GTATAC GT^[AC][GT]AC
AccII 765 CGCG CG^CG
AciI 2789 GCGG CCGC(-3/-1)
AflII 369 CTTAAG C^TTAAG
AflIII 2186 ACACGT A^C[GA][CT]GT
AlwI 1847 GGATC GGATC(4/5)
Asp700I 2558 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 800 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 733 CCCGGG C^[CT]CG[GA]G
AvaII 2777 GGACC G^G[AT]CC
AvrII 3140 CCTAGG C^CTAGG
BbrPI 679 CACGTG CAC^GTG
BbsI 3659 GTCTTC GAAGAC(2/6)
BfrI 369 CTTAAG C^TTAAG
BlnI 3140 CCTAGG C^CTAGG
BpuAI 3659 GTCTTC GAAGAC(2/6)
BsaAI 679 CACGTG [CT]AC^GT[GA]
BsaI 569 GAGACC GGTCTC(1/5)
BseRI 1151 GAGGAG GAGGAG(10/8)
BsmBI 805 CGTCTC CGTCTC(1/5)
BsmI 2266 GAATGC GAATGC(1/-1)
BsoBI 733 CCCGGG C^[CT]CG[GA]G
BspDI 2598 ATCGAT AT^CGAT
BspHI 3205 TCATGA T^CATGA
BsrDI 3433 CATTGC GCAATG(2/0)
Bst1107I 3707 GTATAC GTA^TAC
BstUI 765 CGCG CG^CG
BstZ17I 3707 GTATAC GTA^TAC
CfoI 766 GCGC GCG^C
ClaI 2598 ATCGAT AT^CGAT
EaeI 488 CGGCCA [CT]^GGCC[GA]
HhaI 766 GCGC GCG^C
HinP1I 766 GCGC G^CGC
MvnI 765 CGCG CG^CG
PflFI 800 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 679 CACGTG CAC^GTG
PmlI 679 CACGTG CAC^GTG
RcaI 3205 TCATGA T^CATGA
SapI 3134 GCTCTTC GCTCTTC(1/4)
ScaI 2947 AGTACT AGT^ACT
SmaI 733 CCCGGG CCC^GGG
Tth111I 800 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1129 TCTAGA T^CTAGA
XcmI 3015 CCATAAGAGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 733 CCCGGG C^CCGGG
XmaI 733 CCCGGG C^CCGGG
XmnI 2558 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
Alw44I 229 GTGCAC G^TGCAC
2341 GTGCAC
AlwNI 1520 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
2355 CAGCTCCTG
ApaLI 229 GTGCAC G^TGCAC
2341 GTGCAC
BbvI 289 GCTGC GCAGC(8/12)
2807 GCTGC
BsgI 772 CTGCAC GTGCAG(16/14)
2361 CTGCAC
Bsp1407I 3488 TGTACA T^GTACA
3930 TGTACA
BsrGI 3488 TGTACA T^GTACA
3930 TGTACA
Cfr13I 2777 GGACC G^G[ACGT]CC
3883 GGCCC
EcoT22I 2826 ATGCAT ATGCA^T
2846 ATGCAT
HapII 626 CCGG C^CGG
734 CCGG
HincII 2401 GTCAAC GT[CT]^[GA]AC
3649 GTCAAC
HindII 2401 GTCAAC GT[CT]^[GA]AC
3649 GTCAAC
HindIII 1935 AAGCTT A^AGCTT
2714 AAGCTT
HpaII 626 CCGG C^CGG
734 CCGG
MfeI 2606 CAATTG C^AATTG
3674 CAATTG
MspI 626 CCGG C^CGG
734 CCGG
MunI 2606 CAATTG C^AATTG
3674 CAATTG
MwoI 757 GCTGAGATCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
2807 GCTGCTACTGC
NcoI 1981 CCATGG C^CATGG
3275 CCATGG
NdeI 1259 CATATG CA^TATG
1501 CATATG
NsiI 2826 ATGCAT ATGCA^T
2846 ATGCAT
PflMI 1878 CCAAGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3613 CCACAGGTTGG
PstI 290 CTGCAG CTGCA^G
1627 CTGCAG
Sau96I 2777 GGACC G^G[ACGT]CC
3883 GGCCC
SphI 640 GCATGC GCATG^C
2824 GCATGC
SspBI 3488 TGTACA T^GTACA
3930 TGTACA
TseI 289 GCTGC G^C[AT]GC
2807 GCTGC
Tsp45I 788 GTGAC ^GT[GC]AC
2611 GTCAC
Van91I 1878 CCAAGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3613 CCACAGGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AcsI AfaI AluI ApoI AseI
AsnI AspHI BclI BcnI BfaI
BmyI BpmI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstXI Cac8I
DdeI DpnII DraI DsaI EarI
EcoRII EcoT14I FbaI Fnu4HI FokI
HaeIII HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MslI MvaI NciI
NdeII NlaIII NlaIV NspI PleI
PshBI RsaI Sau3AI ScrFI SfaNI
SfcI SmlI SspI StyI TaqI
TfiI Tru9I Tsp509I TspRI TthHB8I