##################################
# NO CUT ENZYMES ( 49 enzymes) #
##################################
Acc65I AccI AclI AflII AgeI
Aor51HI AscI AseI AsnI Asp718I
BfrI BglII BsaBI BsiWI BspDI
BsrDI BssHII Bst1107I BstAPI BstZ17I
ClaI CpoI Eco47III EcoT22I FseI
HpaI KpnI MamI MfeI MluI
MunI NdeI NheI NotI NruI
NsiI PacI PinAI PmeI PshBI
Psp1406I PvuI RsrII SalI SfiI
SgrAI SnaBI SpeI SwaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AatII 1842 GACGTC GACGT^C
AccII 201 CGCG CG^CG
AhdI 1065 GACGAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 2525 GAAGGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1065 GACGAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 168 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 469 TGCGCA TGC^GCA
AvrII 569 CCTAGG C^CTAGG
BamHI 2643 GGATCC G^GATCC
BbrPI 3423 CACGTG CAC^GTG
BcgI 19 GCATCATCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 569 CCTAGG C^CTAGG
BlpI 1810 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1810 GCTCAGC GC^T[ACGT]AGC
BsmI 2144 GAATGC GAATGC(1/-1)
BsrFI 1649 GCCGGC [GA]^CCGG[CT]
BstUI 201 CGCG CG^CG
CelII 1810 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1649 GCCGGC [GA]^CCGG[CT]
EagI 1651 CGGCCG C^GGCCG
Eam1105I 1065 GACGAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1651 CGGCCG C^GGCCG
Eco52I 1651 CGGCCG C^GGCCG
EcoRI 3502 GAATTC G^AATTC
FspI 469 TGCGCA TGC^GCA
HgaI 1924 GCGTC GACGC(5/10)
HindIII 3243 AAGCTT A^AGCTT
KspI 200 CCGCGG CCGC^GG
MvnI 201 CGCG CG^CG
NaeI 1649 GCCGGC GCC^GGC
NgoMIV 1649 GCCGGC G^CCGGC
PaeR7I 153 CTCGAG C^TCGAG
PflFI 168 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3423 CACGTG CAC^GTG
PmlI 3423 CACGTG CAC^GTG
SacII 200 CCGCGG CCGC^GG
SbfI 1673 CCTGCAGG CCTGCA^GG
SexAI 169 ACCTGGT A^CC[AT]GGT
SphI 3395 GCATGC GCATG^C
Sse8387I 1673 CCTGCAGG CCTGCA^GG
Tth111I 168 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 4084 CCACCAACTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 153 CTCGAG C^TCGAG
XmnI 2525 GAAGGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccIII 244 TCCGGA T^CCGGA
2633 TCCGGA
BalI 1243 TGGCCA TGG^CCA
3788 TGGCCA
BbeI 1728 GGCGCC GGCGC^C
3515 GGCGCC
BbvCI 2767 GCTGAGG CCTCAGC(-5/-2)
2906 GCTGAGG
BciVI 2242 GGATAC GTATCC(6/5)
3736 GTATCC
BclI 2795 TGATCA T^GATCA
4123 TGATCA
BsaAI 3423 CACGTG [CT]AC^GT[GA]
4274 CACGTA
BsaWI 244 TCCGGA [AT]^CCGG[AT]
2633 TCCGGA
BseAI 244 TCCGGA T^CCGGA
2633 TCCGGA
BsiEI 1651 CGGCCG CG[GA][CT]^CG
1775 CGACCG
BsmBI 1844 CGTCTC CGTCTC(1/5)
1722 GAGACG
BspEI 244 TCCGGA T^CCGGA
2633 TCCGGA
BspHI 955 TCATGA T^CATGA
979 TCATGA
BspLU11I 1366 ACATGT A^CATGT
3278 ACATGT
BsrBI 1486 CCGCTC CCGCTC(-3/-3)
1808 CCGCTC
BstBI 1059 TTCGAA TT^CGAA
3232 TTCGAA
DraI 2439 TTTAAA TTT^AAA
2484 TTTAAA
DraIII 1167 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG
3612 CACTGGGTG
EcoRV 951 GATATC GAT^ATC
1535 GATATC
FbaI 2795 TGATCA T^GATCA
4123 TGATCA
KasI 1728 GGCGCC G^GCGCC
3515 GGCGCC
MluNI 1243 TGGCCA TGG^CCA
3788 TGGCCA
MroI 244 TCCGGA T^CCGGA
2633 TCCGGA
MscI 1243 TGGCCA TGG^CCA
3788 TGGCCA
NarI 1728 GGCGCC GG^CGCC
3515 GGCGCC
NspV 1059 TTCGAA TT^CGAA
3232 TTCGAA
PshAI 1760 GACCTCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3588 GACCAAGGTC
RcaI 955 TCATGA T^CATGA
979 TCATGA
SapI 335 GCTCTTC GCTCTTC(1/4)
2690 GCTCTTC
ScaI 1612 AGTACT AGT^ACT
3472 AGTACT
SfoI 1728 GGCGCC GGC^GCC
3515 GGCGCC
SfuI 1059 TTCGAA TT^CGAA
3232 TTCGAA
StuI 2998 AGGCCT AGG^CCT
3664 AGGCCT
XbaI 608 TCTAGA T^CTAGA
2863 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AluI Alw44I AlwI AlwNI AocI
ApaI ApaLI ApoI AspHI AvaI
AvaII BanI BanII BbsI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I Bsp1407I BspMI
BsrGI BsrI BssKI BssSI BstEII
BstF5I BstNI BstPI BstXI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DrdI DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI SspBI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII XmaCI
XmaI