################################## # NO CUT ENZYMES ( 95 enzymes) # ################################## AatII AccII AcyI AhdI Alw44I Aor51HI ApaI ApaLI AscI AspEI AspI AvaI AvrII BbeI BbrPI BbvCI BcgI BciVI BcnI BglI BlnI BsaAI BsaHI BsaI BsiEI BsiWI BsmBI BsoBI BspDI BsrBI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I ClaI CpoI DraII DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoO109I EcoO65I FseI HaeII Hin1I KasI KspI MluI MslI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PmaCI PmeI PmlI PpuMI PshAI PvuI RsrII SacI SacII SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI Sse8387I Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AccIII 526 TCCGGA T^CCGGA AclI 1449 AACGTT AA^CGTT AflII 715 CTTAAG C^TTAAG AflIII 4266 ACATGT A^C[GA][CT]GT AgeI 1150 ACCGGT A^CCGGT AocI 2340 CCTTAGG CC^T[ACGT]AGG AseI 4042 ATTAAT AT^TAAT AsnI 4042 ATTAAT AT^TAAT AviII 2423 TGCGCA TGC^GCA BalI 663 TGGCCA TGG^CCA BanII 761 GAGCCC G[GA]GC[CT]^C BfrI 715 CTTAAG C^TTAAG BsaBI 3844 GATTTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 526 TCCGGA T^CCGGA BsgI 2813 GTGCAG GTGCAG(16/14) BspEI 526 TCCGGA T^CCGGA BspLU11I 4266 ACATGT A^CATGT BsrFI 1150 ACCGGT [GA]^CCGG[CT] BssKI 2501 CCAGG ^CC[ACGT]GG BstNI 2501 CCAGG CC^[AT]GG Bsu36I 2340 CCTTAGG CC^T[ACGT]AGG CfoI 2424 GCGC GCG^C Cfr10I 1150 ACCGGT [GA]^CCGG[CT] DrdI 505 GACTCTCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 663 TGGCCA [CT]^GGCC[GA] EarI 3171 GAAGAG CTCTTC(1/4) Eco81I 2340 CCTTAGG CC^T[ACGT]AGG EcoRI 3407 GAATTC G^AATTC EcoRII 2501 CCAGG ^CC[AT]GG EcoT22I 166 ATGCAT ATGCA^T FauI 2917 GCGGG CCCGC(4/6) FspI 2423 TGCGCA TGC^GCA HgaI 809 GACGC GACGC(5/10) HhaI 2424 GCGC GCG^C HinP1I 2424 GCGC G^CGC HindIII 1284 AAGCTT A^AGCTT HpaI 2827 GTTAAC GTT^AAC Ksp632I 3171 GAAGAG CTCTTC(1/4) MaeII 1450 ACGT A^CGT MamI 3844 GATTTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 667 CAATTG C^AATTG MluNI 663 TGGCCA TGG^CCA MroI 526 TCCGGA T^CCGGA MscI 663 TGGCCA TGG^CCA MunI 667 CAATTG C^AATTG MvaI 2501 CCAGG CC^[AT]GG NsiI 166 ATGCAT ATGCA^T PflMI 4117 CCAATCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 1150 ACCGGT A^CCGGT PshBI 4042 ATTAAT AT^TAAT Psp1406I 1449 AACGTT AA^CGTT SalI 502 GTCGAC G^TCGAC ScrFI 2501 CCAGG CC^[ACGT]GG SpeI 3183 ACTAGT A^CTAGT SphI 2696 GCATGC GCATG^C StuI 2078 AGGCCT AGG^CCT SwaI 3845 ATTTAAAT ATTT^AAAT Van91I 4117 CCAATCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### Acc65I 1199 GGTACC G^GTACC 1519 GGTACC AccI 502 GTCGAC GT^[AC][GT]AC 1220 GTCTAC AlwNI 2704 CAGTTACTG CAG[ACGT][ACGT][ACGT]^CTG 3121 CAGTTTCTG Asp718I 1199 GGTACC G^GTACC 1519 GGTACC AspHI 201 GAGCAC G[AT]GC[AT]^C 4112 GAGCAC AvaII 2067 GGTCC G^G[AT]CC 2601 GGACC BamHI 321 GGATCC G^GATCC 2463 GGATCC BbsI 2533 GAAGAC GAAGAC(2/6) 637 GTCTTC BglII 883 AGATCT A^GATCT 1358 AGATCT BlpI 1395 GCTGAGC GC^T[ACGT]AGC 2573 GCTTAGC BpmI 788 CTGGAG CTGGAG(16/14) 968 CTGGAG Bpu1102I 1395 GCTGAGC GC^T[ACGT]AGC 2573 GCTTAGC BpuAI 2533 GAAGAC GAAGAC(2/6) 637 GTCTTC BsaWI 526 TCCGGA [AT]^CCGG[AT] 1150 ACCGGT BsiHKAI 201 GAGCAC G[AT]GC[AT]^C 4112 GAGCAC BsmI 1545 GAATGC GAATGC(1/-1) 3152 GCATTC BspHI 1005 TCATGA T^CATGA 2447 TCATGA BspMI 3975 ACCTGC ACCTGC(4/8) 1605 GCAGGT BstXI 1308 CCAGAACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2550 CCATTTGATTGG CelII 1395 GCTGAGC GC^T[ACGT]AGC 2573 GCTTAGC DsaI 2100 CCATGG C^C[GA][CT]GG 3533 CCATGG EcoNI 1096 CCTTCATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1770 CCTAGAAAAGG HapII 527 CCGG C^CGG 1151 CCGG HpaII 527 CCGG C^CGG 1151 CCGG KpnI 1199 GGTACC GGTAC^C 1519 GGTACC MspI 527 CCGG C^CGG 1151 CCGG NcoI 2100 CCATGG C^CATGG 3533 CCATGG PstI 3977 CTGCAG CTGCA^G 4153 CTGCAG RcaI 1005 TCATGA T^CATGA 2447 TCATGA ######################################### # 3 OR MORE CUT ENZYMES ( 76 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI Asp700I BanI BbvI BclI BfaI BmyI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BsrDI BsrGI BsrI BstF5I BstYI Cac8I Cfr13I DdeI DpnII DraI EcoRV EcoT14I FbaI Fnu4HI FokI HaeIII HincII HindII HinfI HphI ItaI MaeI MaeIII MboI MboII MflI MnlI MseI MspA1I MwoI NdeII NlaIII NlaIV NspI PleI PvuII RsaI Sau3AI Sau96I ScaI SfaNI SfcI SmlI SspBI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI