##################################
# NO CUT ENZYMES (   95 enzymes) #
##################################

AatII       AccII       AcyI        AhdI        Alw44I      
Aor51HI     ApaI        ApaLI       AscI        AspEI       
AspI        AvaI        AvrII       BbeI        BbrPI       
BbvCI       BcgI        BciVI       BcnI        BglI        
BlnI        BsaAI       BsaHI       BsaI        BsiEI       
BsiWI       BsmBI       BsoBI       BspDI       BsrBI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstZ17I     ClaI        
CpoI        DraII       DraIII      EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoO109I    EcoO65I     
FseI        HaeII       Hin1I       KasI        KspI        
MluI        MslI        MvnI        NaeI        NarI        
NciI        NdeI        NgoMIV      NheI        NotI        
NruI        NspV        PacI        PaeR7I      PflFI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PvuI        RsrII       SacI        SacII       SapI        
SbfI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       Sse8387I    Tth111I     
XbaI        XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   58 enzymes) #
###################################

AccIII        526  TCCGGA               T^CCGGA
AclI         1449  AACGTT               AA^CGTT
AflII         715  CTTAAG               C^TTAAG
AflIII       4266  ACATGT               A^C[GA][CT]GT
AgeI         1150  ACCGGT               A^CCGGT
AocI         2340  CCTTAGG              CC^T[ACGT]AGG
AseI         4042  ATTAAT               AT^TAAT
AsnI         4042  ATTAAT               AT^TAAT
AviII        2423  TGCGCA               TGC^GCA
BalI          663  TGGCCA               TGG^CCA
BanII         761  GAGCCC               G[GA]GC[CT]^C
BfrI          715  CTTAAG               C^TTAAG
BsaBI        3844  GATTTAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI         526  TCCGGA               T^CCGGA
BsgI         2813  GTGCAG               GTGCAG(16/14)
BspEI         526  TCCGGA               T^CCGGA
BspLU11I     4266  ACATGT               A^CATGT
BsrFI        1150  ACCGGT               [GA]^CCGG[CT]
BssKI        2501  CCAGG                ^CC[ACGT]GG
BstNI        2501  CCAGG                CC^[AT]GG
Bsu36I       2340  CCTTAGG              CC^T[ACGT]AGG
CfoI         2424  GCGC                 GCG^C
Cfr10I       1150  ACCGGT               [GA]^CCGG[CT]
DrdI          505  GACTCTCTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI          663  TGGCCA               [CT]^GGCC[GA]
EarI         3171  GAAGAG               CTCTTC(1/4)
Eco81I       2340  CCTTAGG              CC^T[ACGT]AGG
EcoRI        3407  GAATTC               G^AATTC
EcoRII       2501  CCAGG                ^CC[AT]GG
EcoT22I       166  ATGCAT               ATGCA^T
FauI         2917  GCGGG                CCCGC(4/6)
FspI         2423  TGCGCA               TGC^GCA
HgaI          809  GACGC                GACGC(5/10)
HhaI         2424  GCGC                 GCG^C
HinP1I       2424  GCGC                 G^CGC
HindIII      1284  AAGCTT               A^AGCTT
HpaI         2827  GTTAAC               GTT^AAC
Ksp632I      3171  GAAGAG               CTCTTC(1/4)
MaeII        1450  ACGT                 A^CGT
MamI         3844  GATTTAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI          667  CAATTG               C^AATTG
MluNI         663  TGGCCA               TGG^CCA
MroI          526  TCCGGA               T^CCGGA
MscI          663  TGGCCA               TGG^CCA
MunI          667  CAATTG               C^AATTG
MvaI         2501  CCAGG                CC^[AT]GG
NsiI          166  ATGCAT               ATGCA^T
PflMI        4117  CCAATCACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI        1150  ACCGGT               A^CCGGT
PshBI        4042  ATTAAT               AT^TAAT
Psp1406I     1449  AACGTT               AA^CGTT
SalI          502  GTCGAC               G^TCGAC
ScrFI        2501  CCAGG                CC^[ACGT]GG
SpeI         3183  ACTAGT               A^CTAGT
SphI         2696  GCATGC               GCATG^C
StuI         2078  AGGCCT               AGG^CCT
SwaI         3845  ATTTAAAT             ATTT^AAAT
Van91I       4117  CCAATCACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

Acc65I       1199  GGTACC               G^GTACC
             1519  GGTACC              
AccI          502  GTCGAC               GT^[AC][GT]AC
             1220  GTCTAC              
AlwNI        2704  CAGTTACTG            CAG[ACGT][ACGT][ACGT]^CTG
             3121  CAGTTTCTG           
Asp718I      1199  GGTACC               G^GTACC
             1519  GGTACC              
AspHI         201  GAGCAC               G[AT]GC[AT]^C
             4112  GAGCAC              
AvaII        2067  GGTCC                G^G[AT]CC
             2601  GGACC               
BamHI         321  GGATCC               G^GATCC
             2463  GGATCC              
BbsI         2533  GAAGAC               GAAGAC(2/6)
              637  GTCTTC              
BglII         883  AGATCT               A^GATCT
             1358  AGATCT              
BlpI         1395  GCTGAGC              GC^T[ACGT]AGC
             2573  GCTTAGC             
BpmI          788  CTGGAG               CTGGAG(16/14)
              968  CTGGAG              
Bpu1102I     1395  GCTGAGC              GC^T[ACGT]AGC
             2573  GCTTAGC             
BpuAI        2533  GAAGAC               GAAGAC(2/6)
              637  GTCTTC              
BsaWI         526  TCCGGA               [AT]^CCGG[AT]
             1150  ACCGGT              
BsiHKAI       201  GAGCAC               G[AT]GC[AT]^C
             4112  GAGCAC              
BsmI         1545  GAATGC               GAATGC(1/-1)
             3152  GCATTC              
BspHI        1005  TCATGA               T^CATGA
             2447  TCATGA              
BspMI        3975  ACCTGC               ACCTGC(4/8)
             1605  GCAGGT              
BstXI        1308  CCAGAACTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2550  CCATTTGATTGG        
CelII        1395  GCTGAGC              GC^T[ACGT]AGC
             2573  GCTTAGC             
DsaI         2100  CCATGG               C^C[GA][CT]GG
             3533  CCATGG              
EcoNI        1096  CCTTCATCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1770  CCTAGAAAAGG         
HapII         527  CCGG                 C^CGG
             1151  CCGG                
HpaII         527  CCGG                 C^CGG
             1151  CCGG                
KpnI         1199  GGTACC               GGTAC^C
             1519  GGTACC              
MspI          527  CCGG                 C^CGG
             1151  CCGG                
NcoI         2100  CCATGG               C^CATGG
             3533  CCATGG              
PstI         3977  CTGCAG               CTGCA^G
             4153  CTGCAG              
RcaI         1005  TCATGA               T^CATGA
             2447  TCATGA              


#########################################
# 3 OR MORE CUT ENZYMES (   76 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        Asp700I     BanI        BbvI        BclI        
BfaI        BmyI        BsaJI       BseRI       BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    Bsp1407I    
BsrDI       BsrGI       BsrI        BstF5I      BstYI       
Cac8I       Cfr13I      DdeI        DpnII       DraI        
EcoRV       EcoT14I     FbaI        Fnu4HI      FokI        
HaeIII      HincII      HindII      HinfI       HphI        
ItaI        MaeI        MaeIII      MboI        MboII       
MflI        MnlI        MseI        MspA1I      MwoI        
NdeII       NlaIII      NlaIV       NspI        PleI        
PvuII       RsaI        Sau3AI      Sau96I      ScaI        
SfaNI       SfcI        SmlI        SspBI       SspI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XhoII       
XmnI