##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII AclI AcsI AflIII AgeI
ApoI AscI AseI AsnI AspI
AviII AvrII BbrPI BclI BglII
BlnI BsaAI BsaBI BsiWI BsmI
Bsp1407I BspDI BspHI BspLU11I BsrDI
BsrGI BssHII BstBI ClaI CpoI
DraIII EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI MamI MfeI MluI MunI
NdeI NheI NotI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI RsrII SalI SbfI ScaI
SexAI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SspI SwaI Tth111I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AccIII 1992 TCCGGA T^CCGGA
AflII 1910 CTTAAG C^TTAAG
Alw44I 3240 GTGCAC G^TGCAC
Aor51HI 1930 AGCGCT AGC^GCT
ApaLI 3240 GTGCAC G^TGCAC
Asp700I 3125 GAAAGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BcgI 3109 GCACTTCTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1910 CTTAAG C^TTAAG
BseAI 1992 TCCGGA T^CCGGA
BsiEI 1042 CGGCCG CG[GA][CT]^CG
BspEI 1992 TCCGGA T^CCGGA
BssSI 3117 CTCGTG CACGAG(-5/-1)
Bst1107I 3197 GTATAC GTA^TAC
BstEII 59 GGTGACC G^GT[ACGT]ACC
BstPI 59 GGTGACC G^GT[ACGT]ACC
BstZ17I 3197 GTATAC GTA^TAC
DraI 4161 TTTAAA TTT^AAA
DrdI 3479 GACCTCGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1042 CGGCCG C^GGCCG
EclXI 1042 CGGCCG C^GGCCG
Eco47III 1930 AGCGCT AGC^GCT
Eco52I 1042 CGGCCG C^GGCCG
EcoO65I 59 GGTGACC G^GT[ACGT]ACC
KspI 2591 CCGCGG CCGC^GG
MaeII 1824 ACGT A^CGT
MroI 1992 TCCGGA T^CCGGA
SacI 3258 GAGCTC GAGCT^C
SacII 2591 CCGCGG CCGC^GG
SphI 2724 GCATGC GCATG^C
XmnI 3125 GAAAGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 1394 GTCTAC GT^[AC][GT]AC
3197 GTATAC
AhdI 143 GACACAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3510 GACTCGCTGTC
AocI 926 CCTGAGG CC^T[ACGT]AGG
1763 CCTGAGG
AspEI 143 GACACAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3510 GACTCGCTGTC
BbeI 238 GGCGCC GGCGC^C
1859 GGCGCC
BbvCI 1539 CCTCAGC CCTCAGC(-5/-2)
3538 GCTGAGG
BciVI 1314 GGATAC GTATCC(6/5)
4202 GTATCC
BlpI 3729 GCTCAGC GC^T[ACGT]AGC
3847 GCTGAGC
Bpu1102I 3729 GCTCAGC GC^T[ACGT]AGC
3847 GCTGAGC
BsaWI 1992 TCCGGA [AT]^CCGG[AT]
3343 ACCGGA
BsmBI 2429 GAGACG CGTCTC(1/5)
3619 GAGACG
BstAPI 691 GCAAGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2221 GCAGCAGCTGC
Bsu36I 926 CCTGAGG CC^T[ACGT]AGG
1763 CCTGAGG
CelII 3729 GCTCAGC GC^T[ACGT]AGC
3847 GCTGAGC
Eam1105I 143 GACACAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3510 GACTCGCTGTC
Eco81I 926 CCTGAGG CC^T[ACGT]AGG
1763 CCTGAGG
KasI 238 GGCGCC G^GCGCC
1859 GGCGCC
NaeI 1482 GCCGGC GCC^GGC
2867 GCCGGC
NarI 238 GGCGCC GG^CGCC
1859 GGCGCC
NgoMIV 1482 GCCGGC G^CCGGC
2867 GCCGGC
NruI 593 TCGCGA TCG^CGA
3932 TCGCGA
PshAI 1748 GACCCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2072 GACCTGCGTC
PstI 180 CTGCAG CTGCA^G
878 CTGCAG
SfoI 238 GGCGCC GGC^GCC
1859 GGCGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
Acc65I AccII AciI AcyI AfaI
AluI AlwI AlwNI ApaI Asp718I
AspHI AvaI AvaII BalI BamHI
BanI BanII BbsI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KpnI Ksp632I MaeI MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SfiI
SmaI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI