##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII AclI AcsI AflII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
AspEI AspI AviII BbrPI BfrI
BglII BsaAI BsaBI BsaI BsiWI
BsmI BspDI BssHII BstBI Bsu36I
ClaI CpoI DraI DrdI Eam1105I
Eco47III Eco81I EcoRI EcoRV FseI
FspI HincII HindII HpaI MamI
MluI NotI NruI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SalI
SbfI ScaI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspI
SwaI Tth111I XmnI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AccIII 697 TCCGGA T^CCGGA
BciVI 479 GTATCC GTATCC(6/5)
BlpI 2179 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2179 GCTCAGC GC^T[ACGT]AGC
BseAI 697 TCCGGA T^CCGGA
Bsp1407I 2112 TGTACA T^GTACA
BspEI 697 TCCGGA T^CCGGA
BspHI 787 TCATGA T^CATGA
BsrBI 3287 CCGCTC CCGCTC(-3/-3)
BsrDI 1675 CATTGC GCAATG(2/0)
BsrGI 2112 TGTACA T^GTACA
BssSI 1530 CACGAG CACGAG(-5/-1)
Bst1107I 1801 GTATAC GTA^TAC
BstAPI 1832 GCAGCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 1801 GTATAC GTA^TAC
CelII 2179 GCTCAGC GC^T[ACGT]AGC
EagI 75 CGGCCG C^GGCCG
EclXI 75 CGGCCG C^GGCCG
Eco52I 75 CGGCCG C^GGCCG
EcoNI 2017 CCTACCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 2334 ATGCAT ATGCA^T
HindIII 395 AAGCTT A^AGCTT
KspI 196 CCGCGG CCGC^GG
MfeI 3582 CAATTG C^AATTG
MroI 697 TCCGGA T^CCGGA
MunI 3582 CAATTG C^AATTG
NheI 3332 GCTAGC G^CTAGC
NsiI 2334 ATGCAT ATGCA^T
PaeR7I 1713 CTCGAG C^TCGAG
PshAI 2069 GACTACGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 787 TCATGA T^CATGA
SacII 196 CCGCGG CCGC^GG
SapI 3125 GCTCTTC GCTCTTC(1/4)
SexAI 750 ACCAGGT A^CC[AT]GGT
SspBI 2112 TGTACA T^GTACA
XbaI 1360 TCTAGA T^CTAGA
XhoI 1713 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccI 1801 GTATAC GT^[AC][GT]AC
2987 GTCTAC
AlwNI 1833 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
3174 CAGGGCCTG
ApaI 161 GGGCCC GGGCC^C
218 GGGCCC
AvrII 2806 CCTAGG C^CTAGG
3614 CCTAGG
BamHI 526 GGATCC G^GATCC
586 GGATCC
BbvCI 1843 CCTCAGC CCTCAGC(-5/-2)
2668 CCTCAGC
BcgI 1715 CGAGGCCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2392 GCAGGTTTATCG
BclI 933 TGATCA T^GATCA
2422 TGATCA
BlnI 2806 CCTAGG C^CTAGG
3614 CCTAGG
BstXI 2290 CCATGATATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2770 CCAGTGTGCTGG
DraIII 1084 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
1987 CACAGTGTG
FbaI 933 TGATCA T^GATCA
2422 TGATCA
NaeI 73 GCCGGC GCC^GGC
77 GCCGGC
NdeI 2121 CATATG CA^TATG
3317 CATATG
NgoMIV 73 GCCGGC G^CCGGC
77 GCCGGC
PstI 878 CTGCAG CTGCA^G
2390 CTGCAG
SmaI 142 CCCGGG CCC^GGG
169 CCCGGG
TfiI 2471 GAATC G^A[AT]TC
3628 GAATC
XmaCI 142 CCCGGG C^CCGGG
169 CCCGGG
XmaI 142 CCCGGG C^CCGGG
169 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
Acc65I AccII AciI AcyI AfaI
AflIII AluI AlwI Asp718I AspHI
AvaI AvaII BalI BanI BanII
BbeI BbsI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaHI
BsaJI BsaWI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspLU11I BspMI
BsrFI BsrI BssKI BstEII BstF5I
BstNI BstPI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI KpnI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NarI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmlI StuI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII