##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I AspI AviII
BcgI BciVI BclI BfrI BglI
BlpI Bpu1102I BsaBI BsaWI BseAI
BsiEI BsiWI BspDI BspEI BsrFI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I CelII Cfr10I ClaI CpoI
DraI DrdI EagI EclXI Eco47III
Eco52I EcoO65I EcoRV FbaI FseI
FspI HgaI KspI MamI MluI
MroI NaeI NgoMIV NotI NruI
NspV PacI PflFI PinAI PmeI
PshBI Psp1406I PvuI RsrII SacII
SalI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI SspI StuI
SwaI Tth111I XbaI XcmI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 2690 GGTACC G^GTACC
AccI 4038 GTAGAC GT^[AC][GT]AC
AccII 3645 CGCG CG^CG
AcyI 3327 GGCGCC G[GA]^CG[CT]C
AflIII 2758 ACATGT A^C[GA][CT]GT
Asp718I 2690 GGTACC G^GTACC
AvrII 3463 CCTAGG C^CTAGG
BbeI 3327 GGCGCC GGCGC^C
BbrPI 242 CACGTG CAC^GTG
BlnI 3463 CCTAGG C^CTAGG
BsaAI 242 CACGTG [CT]AC^GT[GA]
BsaHI 3327 GGCGCC G[GA]^CG[CT]C
BsmBI 1558 GAGACG CGTCTC(1/5)
BspHI 2074 TCATGA T^CATGA
BspLU11I 2758 ACATGT A^CATGT
BspMI 1860 ACCTGC ACCTGC(4/8)
BssSI 3743 CACGAG CACGAG(-5/-1)
BstAPI 1591 GCAAATTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 3645 CGCG CG^CG
DraIII 3058 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 1111 GAATTC G^AATTC
EcoT22I 2664 ATGCAT ATGCA^T
Hin1I 3327 GGCGCC G[GA]^CG[CT]C
HincII 119 GTTAAC GT[CT]^[GA]AC
HindII 119 GTTAAC GT[CT]^[GA]AC
HindIII 1461 AAGCTT A^AGCTT
HpaI 119 GTTAAC GTT^AAC
KasI 3327 GGCGCC G^GCGCC
KpnI 2690 GGTACC GGTAC^C
MfeI 1936 CAATTG C^AATTG
MunI 1936 CAATTG C^AATTG
MvnI 3645 CGCG CG^CG
NarI 3327 GGCGCC GG^CGCC
NcoI 3250 CCATGG C^CATGG
NdeI 3623 CATATG CA^TATG
NheI 1907 GCTAGC G^CTAGC
NsiI 2664 ATGCAT ATGCA^T
PmaCI 242 CACGTG CAC^GTG
PmlI 242 CACGTG CAC^GTG
PshAI 2757 GACATGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 2074 TCATGA T^CATGA
SbfI 1861 CCTGCAGG CCTGCA^GG
ScaI 4091 AGTACT AGT^ACT
SexAI 2553 ACCAGGT A^CC[AT]GGT
SfoI 3327 GGCGCC GGC^GCC
Sse8387I 1861 CCTGCAGG CCTGCA^GG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AhdI 3236 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3257 GACCTTCTGTC
AspEI 3236 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3257 GACCTTCTGTC
BalI 2093 TGGCCA TGG^CCA
3877 TGGCCA
BamHI 578 GGATCC G^GATCC
820 GGATCC
BbvCI 1613 CCTCAGC CCTCAGC(-5/-2)
3134 GCTGAGG
BcnI 2766 CCGGG CC^[GC]GG
3339 CCGGG
BglII 1573 AGATCT A^GATCT
3391 AGATCT
BsgI 1788 GTGCAG GTGCAG(16/14)
3899 GTGCAG
BsmI 802 GAATGC GAATGC(1/-1)
4106 GAATGC
Bsp1407I 2535 TGTACA T^GTACA
3366 TGTACA
BsrBI 476 CCGCTC CCGCTC(-3/-3)
2336 GAGCGG
BsrDI 256 CATTGC GCAATG(2/0)
3228 GCAATG
BsrGI 2535 TGTACA T^GTACA
3366 TGTACA
BstXI 3832 CCATGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3946 CCACTGCTGTGG
DsaI 2694 CCGTGG C^C[GA][CT]GG
3250 CCATGG
EaeI 2093 TGGCCA [CT]^GGCC[GA]
3877 TGGCCA
Eam1105I 3236 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3257 GACCTTCTGTC
HaeII 3327 GGCGCC [GA]GCGC^[CT]
3847 AGCGCC
MluNI 2093 TGGCCA TGG^CCA
3877 TGGCCA
MscI 2093 TGGCCA TGG^CCA
3877 TGGCCA
NciI 2766 CCGGG CC^[GC]GG
3339 CCGGG
NspI 619 ACATGC [GA]CATG^[CT]
2758 ACATGT
PaeR7I 1415 CTCGAG C^TCGAG
2439 CTCGAG
SspBI 2535 TGTACA T^GTACA
3366 TGTACA
XhoI 1415 CTCGAG C^TCGAG
2439 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI AspHI AvaI
AvaII BanI BanII BbsI BbvI
BfaI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
PflMI PleI PpuMI PstI PvuII
RsaI SacI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII