##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
Acc65I AclI AflII AflIII AgeI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp718I AviII AvrII
BbeI BfrI BlnI BsaI BsiEI
BsiWI BspHI BspLU11I BsrFI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I Cfr10I CpoI EagI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV FseI FspI HaeII HincII
HindII HpaI KasI KpnI MfeI
MluI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SalI ScaI SfiI SfoI SfuI
SgrAI SnaBI SpeI SwaI TfiI
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccII 1934 CGCG CG^CG
AccIII 2197 TCCGGA T^CCGGA
AhdI 6 GACTTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 2030 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 6 GACTTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 1045 GGATCC G^GATCC
BcgI 3166 CGAGGGGCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3277 GTATCC GTATCC(6/5)
BclI 1226 TGATCA T^GATCA
BglII 1711 AGATCT A^GATCT
BsaBI 3515 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2197 TCCGGA [AT]^CCGG[AT]
BseAI 2197 TCCGGA T^CCGGA
BsmI 351 GAATGC GAATGC(1/-1)
Bsp1407I 175 TGTACA T^GTACA
BspDI 687 ATCGAT AT^CGAT
BspEI 2197 TCCGGA T^CCGGA
BsrGI 175 TGTACA T^GTACA
BssSI 1634 CTCGTG CACGAG(-5/-1)
BstUI 1934 CGCG CG^CG
ClaI 687 ATCGAT AT^CGAT
Eam1105I 6 GACTTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 1226 TGATCA T^GATCA
KspI 1933 CCGCGG CCGC^GG
MamI 3515 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2197 TCCGGA T^CCGGA
MvnI 1934 CGCG CG^CG
SacII 1933 CCGCGG CCGC^GG
SapI 2279 GAAGAGC GCTCTTC(1/4)
SbfI 1691 CCTGCAGG CCTGCA^GG
SmaI 885 CCCGGG CCC^GGG
SphI 2395 GCATGC GCATG^C
Sse8387I 1691 CCTGCAGG CCTGCA^GG
SspBI 175 TGTACA T^GTACA
SspI 3788 AATATT AAT^ATT
XmaCI 885 CCCGGG C^CCGGG
XmaI 885 CCCGGG C^CCGGG
XmnI 2030 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AatII 2319 GACGTC GACGT^C
3360 GACGTC
AccI 521 GTCTAC GT^[AC][GT]AC
1548 GTCTAC
AcsI 2088 AAATTT [GA]^AATT[CT]
2460 AAATTC
AcyI 2319 GACGTC G[GA]^CG[CT]C
3360 GACGTC
ApaI 3452 GGGCCC GGGCC^C
3491 GGGCCC
ApoI 2088 AAATTT [GA]^AATT[CT]
2460 AAATTC
AvaI 885 CCCGGG C^[CT]CG[GA]G
3164 CCCGAG
BanI 2684 GGTGCC G^G[CT][GA]CC
3369 GGCACC
BsaHI 2319 GACGTC G[GA]^CG[CT]C
3360 GACGTC
BsoBI 885 CCCGGG C^[CT]CG[GA]G
3164 CCCGAG
BspMI 1947 ACCTGC ACCTGC(4/8)
2350 ACCTGC
BsrBI 741 CCGCTC CCGCTC(-3/-3)
3254 GAGCGG
DrdI 2130 GACATGACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3537 GACGGCATGGTC
EcoT22I 450 ATGCAT ATGCA^T
973 ATGCAT
HgaI 1430 GACGC GACGC(5/10)
3228 GCGTC
Hin1I 2319 GACGTC G[GA]^CG[CT]C
3360 GACGTC
HindIII 1781 AAGCTT A^AGCTT
2243 AAGCTT
NsiI 450 ATGCAT ATGCA^T
973 ATGCAT
SexAI 1465 ACCTGGT A^CC[AT]GGT
2191 ACCTGGT
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AspI AvaII BalI BanII
BbrPI BbsI BbvCI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI Bsp1286I BsrDI BsrI
BssKI BstAPI BstF5I BstNI BstXI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraI DraII DraIII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflFI PflMI PleI PmaCI PmlI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XcmI XhoII