##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AatII AccIII AclI AflII Aor51HI
AscI AseI AsnI AviII AvrII
BcgI BciVI BfrI BlnI BseAI
BsiEI BsiWI BspDI BspEI BsrBI
BssHII BssSI Bst1107I BstZ17I ClaI
CpoI DrdI EagI EclXI Eco47III
Eco52I EcoT22I FseI FspI HpaI
KspI MluI MroI NaeI NgoMIV
NheI NotI NruI NsiI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SacII SbfI ScaI SfiI SgrAI
SpeI Sse8387I SwaI XcmI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 2264 GGTACC G^GTACC
AgeI 2952 ACCGGT A^CCGGT
AhdI 3909 GACTAGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 2264 GGTACC G^GTACC
AspEI 3909 GACTAGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 3380 GGATCC G^GATCC
BbeI 849 GGCGCC GGCGC^C
BclI 124 TGATCA T^GATCA
BglI 601 GCCCCAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 2309 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 720 CGTCTC CGTCTC(1/5)
BsrFI 2952 ACCGGT [GA]^CCGG[CT]
BstBI 3222 TTCGAA TT^CGAA
Cfr10I 2952 ACCGGT [GA]^CCGG[CT]
DraIII 2939 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 3909 GACTAGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 40 GATATC GAT^ATC
FbaI 124 TGATCA T^GATCA
HaeII 849 GGCGCC [GA]GCGC^[CT]
HincII 269 GTCGAC GT[CT]^[GA]AC
HindII 269 GTCGAC GT[CT]^[GA]AC
HindIII 3037 AAGCTT A^AGCTT
KasI 849 GGCGCC G^GCGCC
KpnI 2264 GGTACC GGTAC^C
MamI 2309 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 128 CAATTG C^AATTG
MunI 128 CAATTG C^AATTG
NarI 849 GGCGCC GG^CGCC
NdeI 2228 CATATG CA^TATG
NspV 3222 TTCGAA TT^CGAA
PacI 4056 TTAATTAA TTAAT^TAA
PaeR7I 938 CTCGAG C^TCGAG
PinAI 2952 ACCGGT A^CCGGT
PstI 1483 CTGCAG CTGCA^G
SacI 869 GAGCTC GAGCT^C
SalI 269 GTCGAC G^TCGAC
SapI 221 GCTCTTC GCTCTTC(1/4)
SexAI 4673 ACCTGGT A^CC[AT]GGT
SfoI 849 GGCGCC GGC^GCC
SfuI 3222 TTCGAA TT^CGAA
SnaBI 2629 TACGTA TAC^GTA
SphI 258 GCATGC GCATG^C
SspI 3373 AATATT AAT^ATT
XhoI 938 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 41 enzymes) #
###################################
AccII 1380 CGCG CG^CG
3992 CGCG
AcyI 849 GGCGCC G[GA]^CG[CT]C
1569 GACGCC
Asp700I 218 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2333 GAACAGCTTC
AspI 3283 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3588 GACTTTGTC
BbrPI 467 CACGTG CAC^GTG
1207 CACGTG
BbsI 1566 GAAGAC GAAGAC(2/6)
3931 GAAGAC
BbvCI 4255 GCTGAGG CCTCAGC(-5/-2)
4556 GCTGAGG
BlpI 472 GCTAAGC GC^T[ACGT]AGC
551 GCTCAGC
Bpu1102I 472 GCTAAGC GC^T[ACGT]AGC
551 GCTCAGC
BpuAI 1566 GAAGAC GAAGAC(2/6)
3931 GAAGAC
BsaHI 849 GGCGCC G[GA]^CG[CT]C
1569 GACGCC
BsaWI 2856 TCCGGT [AT]^CCGG[AT]
2952 ACCGGT
Bsp1407I 807 TGTACA T^GTACA
1056 TGTACA
BspHI 375 TCATGA T^CATGA
3290 TCATGA
BspMI 3653 ACCTGC ACCTGC(4/8)
538 GCAGGT
BsrGI 807 TGTACA T^GTACA
1056 TGTACA
BstAPI 1476 GCACCGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3134 GCAGGCCTTGC
BstUI 1380 CGCG CG^CG
3992 CGCG
CelII 472 GCTAAGC GC^T[ACGT]AGC
551 GCTCAGC
CfoI 850 GCGC GCG^C
1381 GCGC
DsaI 874 CCATGG C^C[GA][CT]GG
1099 CCATGG
EcoNI 2235 CCTCACTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3189 CCTGACTCAGG
HhaI 850 GCGC GCG^C
1381 GCGC
Hin1I 849 GGCGCC G[GA]^CG[CT]C
1569 GACGCC
HinP1I 850 GCGC G^CGC
1381 GCGC
MvnI 1380 CGCG CG^CG
3992 CGCG
NcoI 874 CCATGG C^CATGG
1099 CCATGG
PflFI 3283 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3588 GACTTTGTC
PflMI 604 CCAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1448 CCAGGACCTGG
PmaCI 467 CACGTG CAC^GTG
1207 CACGTG
PmlI 467 CACGTG CAC^GTG
1207 CACGTG
RcaI 375 TCATGA T^CATGA
3290 TCATGA
SmaI 531 CCCGGG CCC^GGG
3692 CCCGGG
SspBI 807 TGTACA T^GTACA
1056 TGTACA
TfiI 1458 GATTC G^A[AT]TC
4398 GAATC
Tth111I 3283 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3588 GACTTTGTC
Van91I 604 CCAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1448 CCAGGACCTGG
XbaI 2678 TCTAGA T^CTAGA
2740 TCTAGA
XmaCI 531 CCCGGG C^CCGGG
3692 CCCGGG
XmaI 531 CCCGGG C^CCGGG
3692 CCCGGG
XmnI 218 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2333 GAACAGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI Alw44I AlwI AlwNI AocI
ApaI ApaLI ApoI AspHI AvaI
AvaII BalI BanI BanII BbvI
BcnI BfaI BglII BmyI BpmI
BsaAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspLU11I BsrDI
BsrI BssKI BstEII BstF5I BstNI
BstPI BstXI BstYI Bsu36I Cac8I
Cfr13I DdeI DpnII DraI DraII
EaeI EarI Eco81I EcoO109I EcoO65I
EcoRI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII