##################################
# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AviII BamHI BbeI BbrPI
BciVI BfrI BglI BglII BlpI
Bpu1102I BsaAI BsaHI BseAI BsiEI
BsiWI BsmBI BspDI BspEI BspLU11I
BsrBI BsrFI BssHII BssSI Bst1107I
BstZ17I CelII Cfr10I ClaI CpoI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI FseI FspI HaeII
HgaI Hin1I HpaI KasI KpnI
KspI MluI MroI NaeI NarI
NgoMIV NheI NotI NruI NspI
PacI PaeR7I PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PvuI
RsrII SacII SalI SbfI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SwaI XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccI 1396 GTCTAC GT^[AC][GT]AC
AlwNI 3524 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaI 436 CCCGAG C^[CT]CG[GA]G
BcgI 3619 CGAAAAGGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 405 TGATCA T^GATCA
BsaI 4303 GAGACC GGTCTC(1/5)
BsgI 4464 CTGCAC GTGCAG(16/14)
BsoBI 436 CCCGAG C^[CT]CG[GA]G
BstBI 1576 TTCGAA TT^CGAA
BstEII 507 GGTGACC G^GT[ACGT]ACC
BstPI 507 GGTGACC G^GT[ACGT]ACC
BstXI 4253 CCAGCACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 3877 TTTAAA TTT^AAA
DraII 364 AGGCCCC [GA]G^G[ACGT]CC[CT]
DsaI 3763 CCATGG C^C[GA][CT]GG
EcoO109I 364 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 507 GGTGACC G^GT[ACGT]ACC
EcoRV 1040 GATATC GAT^ATC
FauI 1012 GCGGG CCCGC(4/6)
FbaI 405 TGATCA T^GATCA
HincII 790 GTTGAC GT[CT]^[GA]AC
HindII 790 GTTGAC GT[CT]^[GA]AC
MaeII 873 ACGT A^CGT
MfeI 4333 CAATTG C^AATTG
MspA1I 134 CAGCTG C[AC]G^C[GT]G
MunI 4333 CAATTG C^AATTG
NcoI 3763 CCATGG C^CATGG
NspV 1576 TTCGAA TT^CGAA
PflMI 1108 CCACACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 2860 GACTC GAGTC(4/5)
PvuII 134 CAGCTG CAG^CTG
SacI 604 GAGCTC GAGCT^C
ScaI 3990 AGTACT AGT^ACT
SfuI 1576 TTCGAA TT^CGAA
SmlI 186 CTTGAG C^T[CT][GA]AG
SspI 932 AATATT AAT^ATT
Van91I 1108 CCACACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 268 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AspHI 538 GTGCTC G[AT]GC[AT]^C
604 GAGCTC
AspI 16 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
912 GACTGTGTC
AvaII 844 GGACC G^G[AT]CC
4249 GGTCC
AvrII 533 CCTAGG C^CTAGG
3510 CCTAGG
BalI 807 TGGCCA TGG^CCA
3312 TGGCCA
BanI 3968 GGTGCC G^G[CT][GA]CC
4374 GGTGCC
BbvI 1345 GCAGC GCAGC(8/12)
2226 GCAGC
BcnI 4359 CCGGG CC^[GC]GG
4386 CCCGG
BlnI 533 CCTAGG C^CTAGG
3510 CCTAGG
BsiHKAI 538 GTGCTC G[AT]GC[AT]^C
604 GAGCTC
BstYI 665 GGATCT [GA]^GATC[CT]
858 GGATCT
Cac8I 1893 GCAAGC GC[ACGT]^[ACGT]GC
4042 GCAGGC
CfoI 76 GCGC GCG^C
4229 GCGC
DraIII 756 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
1109 CACACAGTG
EaeI 807 TGGCCA [CT]^GGCC[GA]
3312 TGGCCA
HhaI 76 GCGC GCG^C
4229 GCGC
HinP1I 76 GCGC G^CGC
4229 GCGC
HindIII 1292 AAGCTT A^AGCTT
1318 AAGCTT
MflI 665 GGATCT [GA]^GATC[CT]
858 GGATCT
MluNI 807 TGGCCA TGG^CCA
3312 TGGCCA
MscI 807 TGGCCA TGG^CCA
3312 TGGCCA
NciI 4359 CCGGG CC^[GC]GG
4386 CCCGG
NdeI 3104 CATATG CA^TATG
3380 CATATG
PflFI 16 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
912 GACTGTGTC
PstI 1343 CTGCAG CTGCA^G
4123 CTGCAG
StuI 378 AGGCCT AGG^CCT
3403 AGGCCT
TseI 1345 GCAGC G^C[AT]GC
2226 GCAGC
Tth111I 16 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
912 GACTGTGTC
XhoII 665 GGATCT [GA]^GATC[CT]
858 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AocI ApoI AseI AsnI
Asp700I BanII BbsI BbvCI BfaI
BmyI BpmI BpuAI BsaBI BsaJI
BsaWI BseRI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I Bsp1407I BspHI
BspMI BsrDI BsrGI BsrI BssKI
BstAPI BstF5I BstNI BstUI Bsu36I
Cfr13I DdeI DpnII EarI Eco81I
EcoRI EcoRII EcoT14I EcoT22I Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MamI MboI MboII MnlI MseI
MslI MspI MvaI MvnI MwoI
NdeII NlaIII NlaIV NsiI PshBI
RcaI RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SspBI StyI
TaqI TfiI Tru9I Tsp45I Tsp509I
TspRI TthHB8I XmnI