##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII AccII AccIII AclI Alw44I
AlwNI Aor51HI ApaI ApaLI AscI
AspHI AspI AviII BamHI BcgI
BciVI BglI BglII BseAI BsiEI
BsiHKAI BsiWI BspDI BspEI BsrBI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstYI
BstZ17I ClaI CpoI DsaI EagI
EclXI Eco47III Eco52I EcoO65I FauI
FseI FspI HpaI KspI MflI
MluI MroI MvnI NaeI NcoI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PmeI Psp1406I
PstI PvuI RsrII SacI SacII
SalI SbfI SfiI SfuI SgrAI
SnaBI SphI Sse8387I TaqI Tth111I
TthHB8I XhoI XhoII
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
Acc65I 562 GGTACC G^GTACC
AccI 1531 GTCTAC GT^[AC][GT]AC
AflII 919 CTTAAG C^TTAAG
AgeI 1354 ACCGGT A^CCGGT
AhdI 3928 GACTAAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2360 CCTGAGG CC^T[ACGT]AGG
AseI 3236 ATTAAT AT^TAAT
AsnI 3236 ATTAAT AT^TAAT
Asp718I 562 GGTACC G^GTACC
AspEI 3928 GACTAAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 4272 CCTAGG C^CTAGG
BbeI 506 GGCGCC GGCGC^C
BbrPI 2943 CACGTG CAC^GTG
BclI 2670 TGATCA T^GATCA
BfrI 919 CTTAAG C^TTAAG
BlnI 4272 CCTAGG C^CTAGG
BlpI 1715 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1715 GCTAAGC GC^T[ACGT]AGC
BsaBI 1233 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 1021 GAGGAG GAGGAG(10/8)
BsmBI 3010 GAGACG CGTCTC(1/5)
Bsp1407I 575 TGTACA T^GTACA
BsrFI 1354 ACCGGT [GA]^CCGG[CT]
BsrGI 575 TGTACA T^GTACA
Bsu36I 2360 CCTGAGG CC^T[ACGT]AGG
CelII 1715 GCTAAGC GC^T[ACGT]AGC
CfoI 507 GCGC GCG^C
Cfr10I 1354 ACCGGT [GA]^CCGG[CT]
DrdI 4245 GACTCGGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 3928 GACTAAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2360 CCTGAGG CC^T[ACGT]AGG
FbaI 2670 TGATCA T^GATCA
HaeII 506 GGCGCC [GA]GCGC^[CT]
HhaI 507 GCGC GCG^C
HinP1I 507 GCGC G^CGC
KasI 506 GGCGCC G^GCGCC
KpnI 562 GGTACC GGTAC^C
MamI 1233 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2906 CAATTG C^AATTG
MunI 2906 CAATTG C^AATTG
NarI 506 GGCGCC GG^CGCC
NdeI 3566 CATATG CA^TATG
PinAI 1354 ACCGGT A^CCGGT
PmaCI 2943 CACGTG CAC^GTG
PmlI 2943 CACGTG CAC^GTG
PpuMI 987 AGGTCCC [GA]G^G[AT]CC[CT]
PshAI 3688 GACCTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3236 ATTAAT AT^TAAT
PvuII 3923 CAGCTG CAG^CTG
SapI 1486 GAAGAGC GCTCTTC(1/4)
SfoI 506 GGCGCC GGC^GCC
SmaI 59 CCCGGG CCC^GGG
SpeI 3538 ACTAGT A^CTAGT
SspBI 575 TGTACA T^GTACA
SwaI 3331 ATTTAAAT ATTT^AAAT
XbaI 3532 TCTAGA T^CTAGA
XcmI 2821 CCAAGAAACTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 59 CCCGGG C^CCGGG
XmaI 59 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcyI 162 GACGCC G[GA]^CG[CT]C
506 GGCGCC
AvaI 59 CCCGGG C^[CT]CG[GA]G
4247 CTCGGG
BalI 1235 TGGCCA TGG^CCA
3815 TGGCCA
BsaAI 2287 TACGTG [CT]AC^GT[GA]
2943 CACGTG
BsaHI 162 GACGCC G[GA]^CG[CT]C
506 GGCGCC
BsaWI 392 TCCGGT [AT]^CCGG[AT]
1354 ACCGGT
BsmI 3016 GAATGC GAATGC(1/-1)
3035 GAATGC
BsoBI 59 CCCGGG C^[CT]CG[GA]G
4247 CTCGGG
BsrDI 1467 CATTGC GCAATG(2/0)
1262 GCAATG
DraII 987 AGGTCCC [GA]G^G[ACGT]CC[CT]
4446 AGGGCCT
EcoNI 542 CCTGGGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2788 CCTTGGAAAGG
EcoO109I 987 AGGTCCC [GA]G^G[ACGT]CC[CT]
4446 AGGGCCT
EcoRI 463 GAATTC G^AATTC
2688 GAATTC
EcoRV 1180 GATATC GAT^ATC
3672 GATATC
HgaI 106 GACGC GACGC(5/10)
162 GACGC
Hin1I 162 GACGCC G[GA]^CG[CT]C
506 GGCGCC
MluNI 1235 TGGCCA TGG^CCA
3815 TGGCCA
MscI 1235 TGGCCA TGG^CCA
3815 TGGCCA
MspA1I 41 CAGCGG C[AC]G^C[GT]G
3923 CAGCTG
PflMI 1441 CCAGAATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2328 CCAGCACTTGG
ScaI 1835 AGTACT AGT^ACT
4415 AGTACT
SexAI 530 ACCTGGT A^CC[AT]GGT
677 ACCAGGT
SspI 1954 AATATT AAT^ATT
3467 AATATT
Van91I 1441 CCAGAATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2328 CCAGCACTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI Asp700I AvaII BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaI
BsaJI BsgI BsiYI BslI BsmAI
BsmFI Bsp1286I BspHI BspLU11I BspMI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraI DraIII
EaeI EarI EcoRII EcoT14I EcoT22I
Fnu4HI FokI HaeIII HapII HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NsiI NspI PleI
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XmnI