##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI Aor51HI ApaI AscI
AseI AsnI Asp718I AviII AvrII
BcgI BciVI BclI BfrI BlnI
BsaWI BseAI BsiEI Bsp1407I BspDI
BspEI BsrGI BssHII Bst1107I BstBI
BstZ17I ClaI CpoI DraI DrdI
EagI EclXI Eco47III Eco52I EcoRI
FbaI FseI FspI HpaI KpnI
MluI MroI NheI NotI NruI
NspV PacI PaeR7I PflMI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SalI SbfI SexAI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI Van91I
XcmI XhoI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AhdI 2327 GACACCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 490 GTGCAC G^TGCAC
AocI 4180 CCTGAGG CC^T[ACGT]AGG
ApaLI 490 GTGCAC G^TGCAC
Asp700I 3925 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2327 GACACCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 16 GGATCC G^GATCC
BbeI 2440 GGCGCC GGCGC^C
BbrPI 385 CACGTG CAC^GTG
BbsI 2206 GTCTTC GAAGAC(2/6)
BglI 2606 GCCCCCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 159 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 159 GCTCAGC GC^T[ACGT]AGC
BpuAI 2206 GTCTTC GAAGAC(2/6)
BsaBI 4246 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiWI 1936 CGTACG C^GTACG
BsmBI 1883 GAGACG CGTCTC(1/5)
BspHI 1612 TCATGA T^CATGA
BspLU11I 3304 ACATGT A^CATGT
BsrBI 2383 GAGCGG CCGCTC(-3/-3)
BsrDI 3272 GCAATG GCAATG(2/0)
BssSI 3180 CACGAG CACGAG(-5/-1)
Bsu36I 4180 CCTGAGG CC^T[ACGT]AGG
CelII 159 GCTCAGC GC^T[ACGT]AGC
DraIII 476 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 2327 GACACCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 4180 CCTGAGG CC^T[ACGT]AGG
EcoRV 2023 GATATC GAT^ATC
EcoT22I 2996 ATGCAT ATGCA^T
HaeII 2440 GGCGCC [GA]GCGC^[CT]
HincII 1655 GTCAAC GT[CT]^[GA]AC
HindII 1655 GTCAAC GT[CT]^[GA]AC
KasI 2440 GGCGCC G^GCGCC
MamI 4246 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1577 CAATTG C^AATTG
MunI 1577 CAATTG C^AATTG
NarI 2440 GGCGCC GG^CGCC
NdeI 4035 CATATG CA^TATG
NsiI 2996 ATGCAT ATGCA^T
NspI 3304 ACATGT [GA]CATG^[CT]
PmaCI 385 CACGTG CAC^GTG
PmlI 385 CACGTG CAC^GTG
PpuMI 613 AGGTCCT [GA]G^G[AT]CC[CT]
RcaI 1612 TCATGA T^CATGA
SacI 2306 GAGCTC GAGCT^C
SapI 1681 GCTCTTC GCTCTTC(1/4)
ScaI 696 AGTACT AGT^ACT
SfoI 2440 GGCGCC GGC^GCC
SmaI 3516 CCCGGG CCC^GGG
SspI 234 AATATT AAT^ATT
XbaI 3789 TCTAGA T^CTAGA
XmaCI 3516 CCCGGG C^CCGGG
XmaI 3516 CCCGGG C^CCGGG
XmnI 3925 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AcyI 2264 GACGCC G[GA]^CG[CT]C
2440 GGCGCC
BglII 2100 AGATCT A^GATCT
4119 AGATCT
BsaHI 2264 GACGCC G[GA]^CG[CT]C
2440 GGCGCC
BsaI 177 GAGACC GGTCTC(1/5)
2294 GAGACC
BspMI 3240 ACCTGC ACCTGC(4/8)
3972 GCAGGT
BstAPI 254 GCAAGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
394 GCAGCTGCTGC
BstEII 2566 GGTCACC G^GT[ACGT]ACC
3975 GGTCACC
BstPI 2566 GGTCACC G^GT[ACGT]ACC
3975 GGTCACC
EcoNI 617 CCTCTGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1202 CCTCCCAAAGG
EcoO65I 2566 GGTCACC G^GT[ACGT]ACC
3975 GGTCACC
Hin1I 2264 GACGCC G[GA]^CG[CT]C
2440 GGCGCC
KspI 2226 CCGCGG CCGC^GG
2434 CCGCGG
SacII 2226 CCGCGG CCGC^GG
2434 CCGCGG
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AspI AvaI AvaII BalI BanI
BanII BbvCI BbvI BcnI BfaI
BmyI BpmI BsaAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NcoI NdeII NgoMIV
NlaIII NlaIV PflFI PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I XhoII