##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AccI AclI AflII AgeI AhdI
Aor51HI AscI AseI AsnI Asp700I
AspEI AviII AvrII BbsI BclI
BfrI BglII BlnI BpuAI BsiEI
BsiWI Bsp1407I BspDI BspHI BspLU11I
BsrGI BssHII BssSI Bst1107I BstAPI
BstZ17I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoRI EcoRV EcoT22I FbaI
FseI FspI HpaI MfeI MluI
MunI NcoI NdeI NheI NotI
NruI NsiI NspI PacI PinAI
PmeI PshBI Psp1406I PvuI RcaI
RsrII SalI SapI ScaI SnaBI
SphI SspBI SspI SwaI XbaI
XmnI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
Acc65I 3694 GGTACC G^GTACC
AccIII 2736 TCCGGA T^CCGGA
AflIII 4341 ACGTGT A^C[GA][CT]GT
Asp718I 3694 GGTACC G^GTACC
BalI 770 TGGCCA TGG^CCA
BbeI 956 GGCGCC GGCGC^C
BcgI 2862 CGAGGCGGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3274 GTATCC GTATCC(6/5)
BsaBI 4603 GATGGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2736 TCCGGA [AT]^CCGG[AT]
BseAI 2736 TCCGGA T^CCGGA
BsmI 1574 GAATGC GAATGC(1/-1)
BspEI 2736 TCCGGA T^CCGGA
BstBI 1571 TTCGAA TT^CGAA
BstEII 3771 GGTGACC G^GT[ACGT]ACC
BstPI 3771 GGTGACC G^GT[ACGT]ACC
BstXI 216 CCAGCACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EcoO65I 3771 GGTGACC G^GT[ACGT]ACC
HincII 859 GTTGAC GT[CT]^[GA]AC
HindII 859 GTTGAC GT[CT]^[GA]AC
KasI 956 GGCGCC G^GCGCC
KpnI 3694 GGTACC GGTAC^C
MamI 4603 GATGGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 770 TGGCCA TGG^CCA
MroI 2736 TCCGGA T^CCGGA
MscI 770 TGGCCA TGG^CCA
NarI 956 GGCGCC GG^CGCC
NspV 1571 TTCGAA TT^CGAA
PaeR7I 1499 CTCGAG C^TCGAG
SexAI 4076 ACCAGGT A^CC[AT]GGT
SfiI 762 GGCCAAGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 956 GGCGCC GGC^GCC
SfuI 1571 TTCGAA TT^CGAA
SgrAI 952 CGCCGGCG C[GA]^CCGG[CT]G
SpeI 3510 ACTAGT A^CTAGT
XhoI 1499 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AatII 123 GACGTC GACGT^C
862 GACGTC
AcsI 2785 GAATTT [GA]^AATT[CT]
3280 AAATTT
ApoI 2785 GAATTT [GA]^AATT[CT]
3280 AAATTT
AspI 729 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3774 GACCCTGTC
BamHI 243 GGATCC G^GATCC
4608 GGATCC
BbrPI 2846 CACGTG CAC^GTG
4340 CACGTG
BlpI 3384 GCTCAGC GC^T[ACGT]AGC
3882 GCTAAGC
Bpu1102I 3384 GCTCAGC GC^T[ACGT]AGC
3882 GCTAAGC
BsaAI 2846 CACGTG [CT]AC^GT[GA]
4340 CACGTG
BsrDI 360 GCAATG GCAATG(2/0)
651 GCAATG
BsrI 3951 ACTGG ACTGG(1/-1)
4237 ACTGG
BstYI 243 GGATCC [GA]^GATC[CT]
4608 GGATCC
CelII 3384 GCTCAGC GC^T[ACGT]AGC
3882 GCTAAGC
HaeII 139 AGCGCC [GA]GCGC^[CT]
956 GGCGCC
HindIII 1750 AAGCTT A^AGCTT
2155 AAGCTT
MflI 243 GGATCC [GA]^GATC[CT]
4608 GGATCC
NaeI 27 GCCGGC GCC^GGC
953 GCCGGC
NgoMIV 27 GCCGGC G^CCGGC
953 GCCGGC
PflFI 729 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3774 GACCCTGTC
PflMI 1210 CCAACCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2842 CCATCACGTGG
PmaCI 2846 CACGTG CAC^GTG
4340 CACGTG
PmlI 2846 CACGTG CAC^GTG
4340 CACGTG
PshAI 477 GACCGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3165 GACAAAAGTC
SbfI 573 CCTGCAGG CCTGCA^GG
2997 CCTGCAGG
Sse8387I 573 CCTGCAGG CCTGCA^GG
2997 CCTGCAGG
Tth111I 729 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3774 GACCCTGTC
Van91I 1210 CCAACCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2842 CCATCACGTGG
XhoII 243 GGATCC [GA]^GATC[CT]
4608 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI AlwNI AocI ApaI
ApaLI AspHI AvaI AvaII BanI
BanII BbvCI BbvI BcnI BfaI
BglI BmyI BpmI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BssKI
BstF5I BstNI BstUI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I KspI MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI PstI PvuII
RsaI SacI SacII Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XmaCI XmaI