##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AatII AclI AflII AgeI AhdI
AscI AspEI AvrII BalI BcgI
BclI BfrI BglII BlnI BsaBI
BsiWI BsmI BspDI BspHI BsrFI
BssHII BssSI Cfr10I ClaI CpoI
DraIII Eam1105I EcoT22I FbaI FseI
MamI MluNI MscI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI PacI PaeR7I PinAI PmeI
PshAI Psp1406I PvuI RcaI RsrII
SacI SalI ScaI SfiI SgrAI
SnaBI SpeI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
Acc65I 595 GGTACC G^GTACC
AccIII 2280 TCCGGA T^CCGGA
Alw44I 2147 GTGCAC G^TGCAC
AlwNI 2269 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1796 AGCGCT AGC^GCT
ApaI 3344 GGGCCC GGGCC^C
ApaLI 2147 GTGCAC G^TGCAC
AseI 708 ATTAAT AT^TAAT
AsnI 708 ATTAAT AT^TAAT
Asp718I 595 GGTACC G^GTACC
BbeI 1236 GGCGCC GGCGC^C
BbrPI 3132 CACGTG CAC^GTG
BciVI 1115 GGATAC GTATCC(6/5)
BglI 4095 GCCTCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 2666 GGTCTC GGTCTC(1/5)
BseAI 2280 TCCGGA T^CCGGA
BsiEI 2893 CGGCCG CG[GA][CT]^CG
BsmBI 1946 CGTCTC CGTCTC(1/5)
Bsp1407I 772 TGTACA T^GTACA
BspEI 2280 TCCGGA T^CCGGA
BspLU11I 236 ACATGT A^CATGT
BsrBI 3171 CCGCTC CCGCTC(-3/-3)
BsrDI 1155 GCAATG GCAATG(2/0)
BsrGI 772 TGTACA T^GTACA
BstAPI 4086 GCAACCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2082 TTCGAA TT^CGAA
DrdI 1325 GACAGAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2893 CGGCCG C^GGCCG
EclXI 2893 CGGCCG C^GGCCG
Eco47III 1796 AGCGCT AGC^GCT
Eco52I 2893 CGGCCG C^GGCCG
EcoNI 2043 CCTATGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1707 GAATTC G^AATTC
EcoRV 2477 GATATC GAT^ATC
HindIII 1124 AAGCTT A^AGCTT
KasI 1236 GGCGCC G^GCGCC
KpnI 595 GGTACC GGTAC^C
KspI 2762 CCGCGG CCGC^GG
MfeI 525 CAATTG C^AATTG
MluI 913 ACGCGT A^CGCGT
MroI 2280 TCCGGA T^CCGGA
MunI 525 CAATTG C^AATTG
NarI 1236 GGCGCC GG^CGCC
NspV 2082 TTCGAA TT^CGAA
PflMI 990 CCAAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 3132 CACGTG CAC^GTG
PmlI 3132 CACGTG CAC^GTG
PshBI 708 ATTAAT AT^TAAT
PvuII 2970 CAGCTG CAG^CTG
SacII 2762 CCGCGG CCGC^GG
SapI 3254 GCTCTTC GCTCTTC(1/4)
SbfI 2159 CCTGCAGG CCTGCA^GG
SfoI 1236 GGCGCC GGC^GCC
SfuI 2082 TTCGAA TT^CGAA
SphI 4154 GCATGC GCATG^C
Sse8387I 2159 CCTGCAGG CCTGCA^GG
SspBI 772 TGTACA T^GTACA
StuI 4789 AGGCCT AGG^CCT
Van91I 990 CCAAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 4779 TCTAGA T^CTAGA
XcmI 3064 CCAAGCACAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AflIII 236 ACATGT A^C[GA][CT]GT
913 ACGCGT
AviII 2107 TGCGCA TGC^GCA
3028 TGCGCA
BbvCI 4122 CCTCAGC CCTCAGC(-5/-2)
4249 CCTCAGC
BpmI 966 CTCCAG CTGGAG(16/14)
4055 CTGGAG
BsaAI 86 TACGTG [CT]AC^GT[GA]
3132 CACGTG
BsaWI 1583 ACCGGA [AT]^CCGG[AT]
2280 TCCGGA
BsrI 1398 ACTGG ACTGG(1/-1)
3674 CCAGT
Bst1107I 797 GTATAC GTA^TAC
4687 GTATAC
BstXI 2917 CCAAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4259 CCAAAATTCTGG
BstZ17I 797 GTATAC GTA^TAC
4687 GTATAC
DsaI 2762 CCGCGG C^C[GA][CT]GG
2983 CCACGG
FspI 2107 TGCGCA TGC^GCA
3028 TGCGCA
HpaI 134 GTTAAC GTT^AAC
190 GTTAAC
SexAI 2908 ACCTGGT A^CC[AT]GGT
3223 ACCTGGT
SmaI 2887 CCCGGG CCC^GGG
2991 CCCGGG
XmaCI 2887 CCCGGG C^CCGGG
2991 CCCGGG
XmaI 2887 CCCGGG C^CCGGG
2991 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI AocI ApoI
Asp700I AspHI AspI AvaI AvaII
BamHI BanI BanII BbsI BbvI
BcnI BfaI BlpI BmyI Bpu1102I
BpuAI BsaHI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BssKI BstEII
BstF5I BstNI BstPI BstUI BstYI
Bsu36I Cac8I CelII CfoI Cfr13I
DdeI DpnII DraI DraII EaeI
EarI Eco81I EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PflFI PleI PpuMI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I XhoII
XmnI