##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII Acc65I AclI AgeI Alw44I
AocI Aor51HI ApaI ApaLI AscI
Asp718I AspI AviII BalI BamHI
BanI BbeI BbrPI BclI BglII
BlpI Bpu1102I BsaAI BsaI BseRI
BsiWI BspDI BssHII BssSI Bst1107I
BstEII BstPI BstYI BstZ17I Bsu36I
CelII CfoI ClaI DraIII Eco47III
Eco81I EcoO65I EcoRV FbaI FseI
FspI HaeII HhaI HinP1I KasI
KpnI MflI MluI MluNI MscI
NaeI NarI NcoI NgoMIV NheI
NruI PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI Psp1406I
PvuI SalI SapI SexAI SfoI
SgrAI SnaBI SpeI SphI StuI
Tth111I Van91I XcmI XhoI XhoII
###################################
# ONE CUT ENZYMES ( 62 enzymes) #
###################################
AcyI 18 GGCGTC G[GA]^CG[CT]C
AflII 3470 CTTAAG C^TTAAG
AflIII 1615 ACATGT A^C[GA][CT]GT
AhdI 1761 GACATGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 1761 GACATGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 2883 CCTAGG C^CTAGG
BanII 149 GAGCTC G[GA]GC[CT]^C
BbsI 224 GAAGAC GAAGAC(2/6)
BbvCI 12 GCTGAGG CCTCAGC(-5/-2)
BcgI 1715 CGAACTCTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1834 GGATAC GTATCC(6/5)
BfrI 3470 CTTAAG C^TTAAG
BlnI 2883 CCTAGG C^CTAGG
BpuAI 224 GAAGAC GAAGAC(2/6)
BsaBI 1994 GATAAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 18 GGCGTC G[GA]^CG[CT]C
BsgI 3019 GTGCAG GTGCAG(16/14)
BsiEI 2 CGGCCG CG[GA][CT]^CG
BsmBI 335 GAGACG CGTCTC(1/5)
BsmI 3701 GAATGC GAATGC(1/-1)
Bsp1407I 1103 TGTACA T^GTACA
BspLU11I 1615 ACATGT A^CATGT
BsrBI 37 CCGCTC CCGCTC(-3/-3)
BsrDI 522 GCAATG GCAATG(2/0)
BsrFI 169 GCCGGT [GA]^CCGG[CT]
BsrGI 1103 TGTACA T^GTACA
BstAPI 2293 GCAAAGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2385 TTCGAA TT^CGAA
BstXI 793 CCAGGGCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 169 GCCGGT [GA]^CCGG[CT]
CpoI 171 CGGTCCG CG^G[AT]CCG
DraII 138 AGGACCT [GA]G^G[ACGT]CC[CT]
DrdI 509 GACACAGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2 CGGCCG C^GGCCG
Eam1105I 1761 GACATGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2 CGGCCG C^GGCCG
Eco52I 2 CGGCCG C^GGCCG
EcoNI 1162 CCTAGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 138 AGGACCT [GA]G^G[ACGT]CC[CT]
Hin1I 18 GGCGTC G[GA]^CG[CT]C
HpaI 400 GTTAAC GTT^AAC
KspI 5 CCGCGG CCGC^GG
MaeII 2269 ACGT A^CGT
MamI 1994 GATAAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1790 CAATTG C^AATTG
MunI 1790 CAATTG C^AATTG
NdeI 3706 CATATG CA^TATG
NotI 1 GCGGCCGC GC^GGCCGC
NspV 2385 TTCGAA TT^CGAA
PpuMI 138 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 929 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 171 CGGTCCG CG^G[AT]CCG
SacI 149 GAGCTC GAGCT^C
SacII 5 CCGCGG CCGC^GG
SbfI 142 CCTGCAGG CCTGCA^GG
ScaI 2591 AGTACT AGT^ACT
SfiI 35 GGCCGCTCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 2385 TTCGAA TT^CGAA
Sse8387I 142 CCTGCAGG CCTGCA^GG
SspBI 1103 TGTACA T^GTACA
SwaI 3270 ATTTAAAT ATTT^AAAT
XbaI 3045 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccI 1465 GTCTAC GT^[AC][GT]AC
3077 GTCTAC
AccII 6 CGCG CG^CG
97 CGCG
AccIII 2532 TCCGGA T^CCGGA
3228 TCCGGA
AlwNI 1536 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
2767 CAGTGGCTG
AseI 838 ATTAAT AT^TAAT
1780 ATTAAT
AsnI 838 ATTAAT AT^TAAT
1780 ATTAAT
AvaI 30 CCCGGG C^[CT]CG[GA]G
46 CCCGGG
BglI 36 GCCGCTCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
45 GCCCGGGCGGC
BseAI 2532 TCCGGA T^CCGGA
3228 TCCGGA
BsoBI 30 CCCGGG C^[CT]CG[GA]G
46 CCCGGG
BspEI 2532 TCCGGA T^CCGGA
3228 TCCGGA
BspMI 141 ACCTGC ACCTGC(4/8)
1897 ACCTGC
BstNI 793 CCAGG CC^[AT]GG
2327 CCTGG
BstUI 6 CGCG CG^CG
97 CGCG
EaeI 2 CGGCCG [CT]^GGCC[GA]
1218 CGGCCA
EarI 540 CTCTTC CTCTTC(1/4)
2335 CTCTTC
EcoRI 1354 GAATTC G^AATTC
2928 GAATTC
EcoRII 793 CCAGG ^CC[AT]GG
2327 CCTGG
FauI 89 CCCGC CCCGC(4/6)
7 GCGGG
HgaI 337 GACGC GACGC(5/10)
19 GCGTC
HindIII 1346 AAGCTT A^AGCTT
2409 AAGCTT
Ksp632I 540 CTCTTC CTCTTC(1/4)
2335 CTCTTC
MroI 2532 TCCGGA T^CCGGA
3228 TCCGGA
MslI 1666 CACAATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3706 CATATGTATG
MvaI 793 CCAGG CC^[AT]GG
2327 CCTGG
MvnI 6 CGCG CG^CG
97 CGCG
NspI 376 GCATGT [GA]CATG^[CT]
1615 ACATGT
PleI 2536 GACTC GAGTC(4/5)
3159 GAGTC
PshBI 838 ATTAAT AT^TAAT
1780 ATTAAT
SmaI 30 CCCGGG CCC^GGG
46 CCCGGG
SspI 1884 AATATT AAT^ATT
3508 AATATT
XmaCI 30 CCCGGG C^CCGGG
46 CCCGGG
XmaI 30 CCCGGG C^CCGGG
46 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AspHI AvaII BbvI
BcnI BfaI BmyI BpmI BsaJI
BsaWI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspHI BsrI BssKI
BstF5I Cac8I Cfr13I DdeI DpnII
DraI DsaI EcoT14I EcoT22I Fnu4HI
FokI HaeIII HapII HincII HindII
HinfI HpaII HphI ItaI MaeI
MaeIII MboI MboII MnlI MseI
MspA1I MspI MwoI NciI NdeII
NlaIII NlaIV NsiI PstI PvuII
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XmnI