##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII Acc65I AclI AflII AhdI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvrII BciVI BfrI
BglII BlnI BsaBI BsiWI BspDI
BspLU11I BsrDI BstBI BstEII BstPI
ClaI CpoI DraI DrdI Eam1105I
EcoO65I EcoRI EcoT22I FseI HpaI
KpnI MamI MfeI MunI NotI
NruI NsiI NspI NspV PacI
PflFI PmeI PshAI PshBI Psp1406I
PvuI RsrII ScaI SfuI SgrAI
SnaBI SpeI SphI SspI SwaI
Tth111I XmnI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccIII 253 TCCGGA T^CCGGA
AgeI 2297 ACCGGT A^CCGGT
Alw44I 2520 GTGCAC G^TGCAC
AocI 1354 CCTAAGG CC^T[ACGT]AGG
ApaLI 2520 GTGCAC G^TGCAC
AviII 539 TGCGCA TGC^GCA
BamHI 3777 GGATCC G^GATCC
BcgI 291 GCAGGCTGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 3036 GCCTGAACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BseAI 253 TCCGGA T^CCGGA
BsmI 428 GCATTC GAATGC(1/-1)
Bsp1407I 965 TGTACA T^GTACA
BspEI 253 TCCGGA T^CCGGA
BsrBI 1846 GAGCGG CCGCTC(-3/-3)
BsrGI 965 TGTACA T^GTACA
BssHII 72 GCGCGC G^CGCGC
Bst1107I 937 GTATAC GTA^TAC
BstAPI 678 GCAGAATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 2084 CCACTATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 937 GTATAC GTA^TAC
Bsu36I 1354 CCTAAGG CC^T[ACGT]AGG
EagI 3912 CGGCCG C^GGCCG
EclXI 3912 CGGCCG C^GGCCG
Eco52I 3912 CGGCCG C^GGCCG
Eco81I 1354 CCTAAGG CC^T[ACGT]AGG
EcoRV 2755 GATATC GAT^ATC
FspI 539 TGCGCA TGC^GCA
HindIII 268 AAGCTT A^AGCTT
MluI 469 ACGCGT A^CGCGT
MroI 253 TCCGGA T^CCGGA
NdeI 3884 CATATG CA^TATG
NheI 1407 GCTAGC G^CTAGC
PinAI 2297 ACCGGT A^CCGGT
SacI 436 GAGCTC GAGCT^C
SalI 1041 GTCGAC G^TCGAC
SbfI 288 CCTGCAGG CCTGCA^GG
SexAI 305 ACCTGGT A^CC[AT]GGT
SfiI 3035 GGCCTGAACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 288 CCTGCAGG CCTGCA^GG
SspBI 965 TGTACA T^GTACA
XbaI 858 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 937 GTATAC GT^[AC][GT]AC
1041 GTCGAC
AcsI 681 GAATTT [GA]^AATT[CT]
3118 AAATTC
ApoI 681 GAATTT [GA]^AATT[CT]
3118 AAATTC
BbrPI 761 CACGTG CAC^GTG
2740 CACGTG
BclI 984 TGATCA T^GATCA
1580 TGATCA
BsaWI 253 TCCGGA [AT]^CCGG[AT]
2297 ACCGGT
BspHI 818 TCATGA T^CATGA
3083 TCATGA
BspMI 777 GCAGGT ACCTGC(4/8)
1787 GCAGGT
BssSI 793 CACGAG CACGAG(-5/-1)
2824 CACGAG
BstYI 2495 AGATCC [GA]^GATC[CT]
3777 GGATCC
DraIII 712 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG
3680 CACCCTGTG
FbaI 984 TGATCA T^GATCA
1580 TGATCA
MflI 2495 AGATCC [GA]^GATC[CT]
3777 GGATCC
NaeI 34 GCCGGC GCC^GGC
3595 GCCGGC
NgoMIV 34 GCCGGC G^CCGGC
3595 GCCGGC
PaeR7I 2704 CTCGAG C^TCGAG
3784 CTCGAG
PleI 1108 GACTC GAGTC(4/5)
3271 GACTC
PmaCI 761 CACGTG CAC^GTG
2740 CACGTG
PmlI 761 CACGTG CAC^GTG
2740 CACGTG
RcaI 818 TCATGA T^CATGA
3083 TCATGA
StuI 2810 AGGCCT AGG^CCT
2993 AGGCCT
XcmI 2684 CCATCGTGTCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3137 CCACATCCTCACTGG
XhoI 2704 CTCGAG C^TCGAG
3784 CTCGAG
XhoII 2495 AGATCC [GA]^GATC[CT]
3777 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI Aor51HI ApaI
AspHI AvaI AvaII BalI BanI
BanII BbeI BbsI BbvCI BbvI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BsrFI BsrI BssKI
BstF5I BstNI BstUI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco47III
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
KasI Ksp632I KspI MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NcoI NdeII NlaIII NlaIV PflMI
PpuMI PstI PvuII RsaI SacII
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XmaCI
XmaI