##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII AccIII AclI AgeI AhdI
Alw44I ApaLI AscI AseI AsnI
AspEI AviII BbrPI BcgI BciVI
BlpI Bpu1102I BsaWI BseAI BsiWI
BsmBI Bsp1407I BspDI BspEI BsrGI
BssHII Bst1107I BstAPI BstZ17I CelII
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco52I EcoRV FseI FspI
MroI NaeI NdeI NgoMIV NheI
NotI NruI PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII ScaI SexAI
SfiI SgrAI SnaBI SpeI SphI
SspBI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
ApaI 6202 GGGCCC GGGCC^C
AspI 2828 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 5623 GCTGAGG CCTCAGC(-5/-2)
BsiEI 6070 CGGTCG CG[GA][CT]^CG
BsmI 5554 GCATTC GAATGC(1/-1)
BspHI 1107 TCATGA T^CATGA
BspLU11I 4972 ACATGT A^CATGT
BsrFI 5303 ACCGGC [GA]^CCGG[CT]
BssSI 5016 CTCGTG CACGAG(-5/-1)
BstBI 849 TTCGAA TT^CGAA
BstEII 1750 GGTGACC G^GT[ACGT]ACC
BstPI 1750 GGTGACC G^GT[ACGT]ACC
Cfr10I 5303 ACCGGC [GA]^CCGG[CT]
DraIII 5013 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 1750 GGTGACC G^GT[ACGT]ACC
MfeI 936 CAATTG C^AATTG
MluI 4032 ACGCGT A^CGCGT
MunI 936 CAATTG C^AATTG
NspV 849 TTCGAA TT^CGAA
PflFI 2828 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 1107 TCATGA T^CATGA
SacI 2112 GAGCTC GAGCT^C
SalI 1217 GTCGAC G^TCGAC
SbfI 3191 CCTGCAGG CCTGCA^GG
SfuI 849 TTCGAA TT^CGAA
SmaI 1377 CCCGGG CCC^GGG
Sse8387I 3191 CCTGCAGG CCTGCA^GG
StuI 652 AGGCCT AGG^CCT
Tth111I 2828 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 1377 CCCGGG C^CCGGG
XmaI 1377 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
Acc65I 413 GGTACC G^GTACC
3205 GGTACC
AcyI 10 GGCGCC G[GA]^CG[CT]C
1790 GGCGCC
AflII 2241 CTTAAG C^TTAAG
2750 CTTAAG
AflIII 4032 ACGCGT A^C[GA][CT]GT
4972 ACATGT
AocI 1812 CCTGAGG CC^T[ACGT]AGG
2424 CCTGAGG
Aor51HI 3971 AGCGCT AGC^GCT
4645 AGCGCT
Asp700I 1471 GAAACTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2695 GAAAGTTTTC
Asp718I 413 GGTACC G^GTACC
3205 GGTACC
AvaI 1377 CCCGGG C^[CT]CG[GA]G
6284 CTCGGG
BamHI 2004 GGATCC G^GATCC
6767 GGATCC
BbeI 10 GGCGCC GGCGC^C
1790 GGCGCC
BcnI 1377 CCCGG CC^[GC]GG
1378 CCGGG
BfrI 2241 CTTAAG C^TTAAG
2750 CTTAAG
BglI 2 GCCGCTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
5426 GCCATATAGGC
BglII 1101 AGATCT A^GATCT
5714 AGATCT
BsaAI 5802 CACGTA [CT]AC^GT[GA]
6476 CACGTA
BsaBI 838 GATTCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5928 GATATTCATC
BsaHI 10 GGCGCC G[GA]^CG[CT]C
1790 GGCGCC
BsoBI 1377 CCCGGG C^[CT]CG[GA]G
6284 CTCGGG
Bsu36I 1812 CCTGAGG CC^T[ACGT]AGG
2424 CCTGAGG
Eco47III 3971 AGCGCT AGC^GCT
4645 AGCGCT
Eco81I 1812 CCTGAGG CC^T[ACGT]AGG
2424 CCTGAGG
HgaI 6034 GACGC GACGC(5/10)
117 GCGTC
Hin1I 10 GGCGCC G[GA]^CG[CT]C
1790 GGCGCC
HpaI 482 GTTAAC GTT^AAC
1840 GTTAAC
KasI 10 GGCGCC G^GCGCC
1790 GGCGCC
KpnI 413 GGTACC GGTAC^C
3205 GGTACC
MamI 838 GATTCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5928 GATATTCATC
NarI 10 GGCGCC GG^CGCC
1790 GGCGCC
NciI 1377 CCCGG CC^[GC]GG
1378 CCGGG
NcoI 564 CCATGG C^CATGG
6198 CCATGG
NspI 3899 ACATGC [GA]CATG^[CT]
4972 ACATGT
SfoI 10 GGCGCC GGC^GCC
1790 GGCGCC
SspI 3667 AATATT AAT^ATT
5410 AATATT
XmnI 1471 GAAACTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2695 GAAAGTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaII AvrII BalI BanI BanII
BbsI BbvI BclI BfaI BlnI
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrBI
BsrDI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRI EcoRII EcoT14I EcoT22I FauI
FbaI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NdeII NlaIII
NlaIV NsiI PflMI PleI PpuMI
PstI PvuII RsaI SacII SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII