##################################
# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII Acc65I AccIII AclI AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BalI BamHI BbeI BbrPI BbvCI
BcgI BclI BglI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsmI BspDI BspEI BspHI BspLU11I
BspMI BsrDI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV FauI
FbaI FseI FspI HaeII HpaI
KasI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MspA1I
MunI NaeI NarI NcoI NgoMIV
NheI NotI NruI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
StuI SwaI Tth111I XbaI XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccI 490 GTAGAC GT^[AC][GT]AC
AccII 177 CGCG CG^CG
AcyI 1145 GACGCC G[GA]^CG[CT]C
AflII 439 CTTAAG C^TTAAG
AlwNI 1745 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
AvaI 91 CTCGGG C^[CT]CG[GA]G
BanI 214 GGTGCC G^G[CT][GA]CC
BciVI 3 GGATAC GTATCC(6/5)
BfrI 439 CTTAAG C^TTAAG
BglII 1411 AGATCT A^GATCT
BpmI 74 CTGGAG CTGGAG(16/14)
BsaHI 1145 GACGCC G[GA]^CG[CT]C
BsmFI 1025 GGGAC GGGAC(10/14)
BsoBI 91 CTCGGG C^[CT]CG[GA]G
Bsp1407I 1282 TGTACA T^GTACA
BsrBI 148 GAGCGG CCGCTC(-3/-3)
BsrGI 1282 TGTACA T^GTACA
BstUI 177 CGCG CG^CG
CfoI 178 GCGC GCG^C
DraI 1968 TTTAAA TTT^AAA
DraII 1856 AGGGCCT [GA]G^G[ACGT]CC[CT]
EaeI 1389 TGGCCG [CT]^GGCC[GA]
EcoO109I 1856 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 846 CCTTGG C^C[AT][AT]GG
EcoT22I 1241 ATGCAT ATGCA^T
HgaI 1145 GACGC GACGC(5/10)
HhaI 178 GCGC GCG^C
Hin1I 1145 GACGCC G[GA]^CG[CT]C
HinP1I 178 GCGC G^CGC
HincII 1045 GTTGAC GT[CT]^[GA]AC
HindII 1045 GTTGAC GT[CT]^[GA]AC
MaeII 309 ACGT A^CGT
MvnI 177 CGCG CG^CG
NsiI 1241 ATGCAT ATGCA^T
PflMI 1827 CCAGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 44 GACTC GAGTC(4/5)
SfcI 1100 CTGTAG C^T[GA][CT]AG
SspBI 1282 TGTACA T^GTACA
SspI 710 AATATT AAT^ATT
StyI 846 CCTTGG C^C[AT][AT]GG
Tsp45I 1648 GTCAC ^GT[GC]AC
Van91I 1827 CCAGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AciI 129 CCGC CCGC(-3/-1)
150 GCGG
AvaII 843 GGACC G^G[AT]CC
1201 GGACC
BanII 571 GAGCTC G[GA]GC[CT]^C
1352 GAGCTC
BcnI 23 CCCGG CC^[GC]GG
144 CCGGG
DsaI 1392 CCGTGG C^C[GA][CT]GG
1452 CCGTGG
HindIII 1890 AAGCTT A^AGCTT
1941 AAGCTT
HphI 26 GGTGA GGTGA(8/7)
2075 GGTGA
NciI 23 CCCGG CC^[GC]GG
144 CCGGG
NdeI 401 CATATG CA^TATG
1238 CATATG
SacI 571 GAGCTC GAGCT^C
1352 GAGCTC
ScaI 87 AGTACT AGT^ACT
430 AGTACT
SfaNI 728 GCATC GCATC(5/9)
1243 GCATC
SmlI 439 CTTAAG C^T[CT][GA]AG
1727 CTCAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
Asp700I AspHI BbsI BbvI BfaI
BmyI BpuAI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII EarI EcoRII
Fnu4HI FokI HaeIII HapII HinfI
HpaII ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MspI MvaI MwoI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I XhoII XmnI