##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII Acc65I AccIII AgeI AhdI
ApaI AscI Asp718I AspEI AvrII
BciVI BclI BlnI BsaBI BseAI
BsiWI BspDI BspEI BspHI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI DraIII EagI Eam1105I
EclXI Eco52I EcoO65I EcoRV FbaI
FseI KpnI KspI MamI MroI
NheI NotI NruI NspV PacI
PflMI PinAI PmeI PvuI RcaI
RsrII SacII SalI SapI SbfI
SexAI SfiI SfuI SpeI SphI
Sse8387I SspI Van91I XbaI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AccI 2461 GTAGAC GT^[AC][GT]AC
AflII 5214 CTTAAG C^TTAAG
Aor51HI 1044 AGCGCT AGC^GCT
Asp700I 3651 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbrPI 3339 CACGTG CAC^GTG
BfrI 5214 CTTAAG C^TTAAG
BglI 1029 GCCATCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 4871 AGATCT A^GATCT
BsaI 4587 GGTCTC GGTCTC(1/5)
BsiEI 1492 CGGTCG CG[GA][CT]^CG
BsrBI 23 CCGCTC CCGCTC(-3/-3)
Eco47III 1044 AGCGCT AGC^GCT
EcoNI 3672 CCTCCCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 217 CCATGG C^C[AT][AT]GG
MfeI 5200 CAATTG C^AATTG
MluI 2049 ACGCGT A^CGCGT
MunI 5200 CAATTG C^AATTG
NcoI 217 CCATGG C^CATGG
NdeI 5569 CATATG CA^TATG
PaeR7I 1915 CTCGAG C^TCGAG
PmaCI 3339 CACGTG CAC^GTG
PmlI 3339 CACGTG CAC^GTG
PshAI 2690 GACCCACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 3534 CAGCTG CAG^CTG
ScaI 4206 AGTACT AGT^ACT
SgrAI 60 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 5230 CCCGGG CCC^GGG
SnaBI 4946 TACGTA TAC^GTA
StyI 217 CCATGG C^C[AT][AT]GG
SwaI 4320 ATTTAAAT ATTT^AAAT
XhoI 1915 CTCGAG C^TCGAG
XmaCI 5230 CCCGGG C^CCGGG
XmaI 5230 CCCGGG C^CCGGG
XmnI 3651 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AclI 3508 AACGTT AA^CGTT
5128 AACGTT
Alw44I 1776 GTGCAC G^TGCAC
2679 GTGCAC
AocI 1803 CCTCAGG CC^T[ACGT]AGG
4643 CCTGAGG
ApaLI 1776 GTGCAC G^TGCAC
2679 GTGCAC
AseI 209 ATTAAT AT^TAAT
1608 ATTAAT
AsnI 209 ATTAAT AT^TAAT
1608 ATTAAT
AviII 2131 TGCGCA TGC^GCA
4017 TGCGCA
BamHI 1248 GGATCC G^GATCC
1596 GGATCC
BcgI 4631 CGATGTGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1884 GCAGCAGCCTCG
BsaAI 3339 CACGTG [CT]AC^GT[GA]
4946 TACGTA
BsaWI 1205 ACCGGA [AT]^CCGG[AT]
3433 ACCGGA
Bsp1407I 2157 TGTACA T^GTACA
3328 TGTACA
BspLU11I 4352 ACATGT A^CATGT
5405 ACATGT
BsrGI 2157 TGTACA T^GTACA
3328 TGTACA
BstXI 2201 CCATGCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3262 CCAAGAAAGTGG
Bsu36I 1803 CCTCAGG CC^T[ACGT]AGG
4643 CCTGAGG
DrdI 992 GACAAAGTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3615 GACACTAAGGTC
Eco81I 1803 CCTCAGG CC^T[ACGT]AGG
4643 CCTGAGG
FspI 2131 TGCGCA TGC^GCA
4017 TGCGCA
HgaI 2048 GACGC GACGC(5/10)
1401 GCGTC
HindIII 683 AAGCTT A^AGCTT
1957 AAGCTT
HpaI 4200 GTTAAC GTT^AAC
4504 GTTAAC
MspA1I 1141 CCGCTG C[AC]G^C[GT]G
3534 CAGCTG
PpuMI 3553 AGGACCC [GA]G^G[AT]CC[CT]
3622 AGGTCCC
PshBI 209 ATTAAT AT^TAAT
1608 ATTAAT
Psp1406I 3508 AACGTT AA^CGTT
5128 AACGTT
SspBI 2157 TGTACA T^GTACA
3328 TGTACA
StuI 84 AGGCCT AGG^CCT
3669 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
AspHI AspI AvaI AvaII BalI
BanI BanII BbeI BbsI BbvCI
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1286I BspMI BsrDI BsrFI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstUI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI EcoO109I
EcoRI EcoRII EcoT22I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspI MvaI MvnI MwoI
NaeI NarI NciI NdeII NgoMIV
NlaIII NlaIV NsiI NspI PflFI
PleI PstI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmlI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
XcmI XhoII