##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AccIII AclI AflIII AgeI Aor51HI
AscI AseI AsnI AviII AvrII
BbeI BbrPI BcgI BlnI BsaAI
BsaBI BseAI BsiWI BsmBI BspEI
BspHI BspLU11I BssHII BssSI BstBI
CpoI DrdI EagI EclXI Eco47III
Eco52I EcoT22I FseI FspI HincII
HindII HindIII HpaI KasI KspI
MamI MluI MroI NarI NcoI
NdeI NotI NsiI NspI NspV
PacI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspI
SwaI XbaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AatII 2299 GACGTC GACGT^C
Acc65I 2622 GGTACC G^GTACC
AccI 3690 GTATAC GT^[AC][GT]AC
AflII 3556 CTTAAG C^TTAAG
AhdI 1936 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2110 GTGCAC G^TGCAC
ApaLI 2110 GTGCAC G^TGCAC
Asp700I 3654 GAAAGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2622 GGTACC G^GTACC
AspEI 1936 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2128 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 2802 TGGCCA TGG^CCA
BamHI 424 GGATCC G^GATCC
BbvCI 608 CCTCAGC CCTCAGC(-5/-2)
BciVI 2956 GGATAC GTATCC(6/5)
BfrI 3556 CTTAAG C^TTAAG
BglI 3008 GCCCTGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1187 AGATCT A^GATCT
BsmI 1441 GAATGC GAATGC(1/-1)
Bsp1407I 2996 TGTACA T^GTACA
BspDI 2791 ATCGAT AT^CGAT
BsrGI 2996 TGTACA T^GTACA
Bst1107I 3690 GTATAC GTA^TAC
BstAPI 2880 GCAGCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1659 GGTGACC G^GT[ACGT]ACC
BstPI 1659 GGTGACC G^GT[ACGT]ACC
BstZ17I 3690 GTATAC GTA^TAC
ClaI 2791 ATCGAT AT^CGAT
DraI 770 TTTAAA TTT^AAA
DsaI 76 CCACGG C^C[GA][CT]GG
EaeI 2802 TGGCCA [CT]^GGCC[GA]
Eam1105I 1936 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1659 GGTGACC G^GT[ACGT]ACC
EcoRI 3460 GAATTC G^AATTC
KpnI 2622 GGTACC GGTAC^C
MfeI 4048 CAATTG C^AATTG
MluNI 2802 TGGCCA TGG^CCA
MscI 2802 TGGCCA TGG^CCA
MunI 4048 CAATTG C^AATTG
NheI 1179 GCTAGC G^CTAGC
NruI 206 TCGCGA TCG^CGA
PaeR7I 951 CTCGAG C^TCGAG
PflFI 2128 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacI 3865 GAGCTC GAGCT^C
SmaI 3590 CCCGGG CCC^GGG
SspBI 2996 TGTACA T^GTACA
StuI 3116 AGGCCT AGG^CCT
TfiI 581 GAATC G^A[AT]TC
Tth111I 2128 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 880 CCACAGCAGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 951 CTCGAG C^TCGAG
XmaCI 3590 CCCGGG C^CCGGG
XmaI 3590 CCCGGG C^CCGGG
XmnI 3654 GAAAGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 3460 GAATTC [GA]^AATT[CT]
3738 GAATTT
AcyI 2299 GACGTC G[GA]^CG[CT]C
3343 GGCGTC
AlwNI 2215 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
2534 CAGGGGCTG
ApoI 3460 GAATTC [GA]^AATT[CT]
3738 GAATTT
BclI 488 TGATCA T^GATCA
2769 TGATCA
BfaI 1180 CTAG C^TAG
2576 CTAG
BlpI 2139 GCTGAGC GC^T[ACGT]AGC
3762 GCTCAGC
Bpu1102I 2139 GCTGAGC GC^T[ACGT]AGC
3762 GCTCAGC
BsaHI 2299 GACGTC G[GA]^CG[CT]C
3343 GGCGTC
BsiEI 1098 CGACCG CG[GA][CT]^CG
2340 CGACCG
BsrDI 803 CATTGC GCAATG(2/0)
1560 CATTGC
CelII 2139 GCTGAGC GC^T[ACGT]AGC
3762 GCTCAGC
DraIII 904 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
2113 CACAAGGTG
EarI 1363 CTCTTC CTCTTC(1/4)
3253 GAAGAG
EcoNI 3723 CCTGCAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3801 CCTCCCCCAGG
EcoRV 1153 GATATC GAT^ATC
1921 GATATC
FbaI 488 TGATCA T^GATCA
2769 TGATCA
HaeII 1728 GGCGCT [GA]GCGC^[CT]
2255 GGCGCT
Hin1I 2299 GACGTC G[GA]^CG[CT]C
3343 GGCGTC
Ksp632I 1363 CTCTTC CTCTTC(1/4)
3253 GAAGAG
MaeI 1180 CTAG C^TAG
2576 CTAG
PshAI 1074 GACTCGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2341 GACCGGAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AocI ApaI AspHI AvaI AvaII
BanI BanII BbsI BbvI BcnI
BmyI BpmI BpuAI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NdeII
NgoMIV NlaIII NlaIV PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII